Results: 30

PCR interna con ADN extraído directamente de portaobjetos positivos para confirmar o excluir el diagnóstico de tuberculosis: enfoque en la bioseguridad
In-house PCR with DNA extracted directly from positive slides to confirm or exclude the diagnosis of tuberculosis: focus on biosafety

Rev. argent. microbiol; 47 (1), 2015
The possibility to obtain DNA from smears is a valuable alternative to remedy the lack of samples when they are totally used for bacilloscopy; this technique solves the biosafety problem related to a possible accident with the transportation of flasks containing potentially transmissible clinical samples...

Espectro de mutaciones en el gen CFTR en muestras de ADN de pacientes del noreste argentino
Spectrum in CFTR gene mutations in patient DNA samples collected from the argentine northeast

INTRODUCCIÓN La frecuencia de mutaciones causantes de fibrosis quística (FQ) en el gen CFTR presenta diferentes valores en distintas poblaciones del mundo. Existen escasos datos reportados sobre mutaciones halladas en pacientes fibroquísticos del noreste argentino (NEA). OBJETIVOS Estudiar 29 de las m...

Carga parasitaria determinada por PCR en tiempo real en madres serorreactivas contra trypanosoma cruzi
Parasite load determined by real time PCR in seroreactive mothers against trypanosoma cruzi

INTRODUCCIÓN Una vez que se ha logrado el control vectorial y transfusional, la transmisión congénita por Trypanosoma cruzi (T. cruzi) es la vía y la fuente principal de nuevos casos de enfermedad de Chagas en América Latina y en países no endémicos. OBJETIVOS Evaluar la parasitemia de las madres ...

Evaluación cuantitativa del riesgo de organismos virales en aguas de consumo: estudio en poblaciones semirrurales de la provincia de Salta
Quantitative evaluation of the risk of viruses in drinking water: study in semi-rural populations of the province of Salta

INTRODUCCIÓN En Argentina, para el control de la contaminación microbiana de las aguas se utilizan indicadores bacterianos. Sin embargo, estos indicadores no revelan satisfactoriamente la calidad viral de las aguas. OBJETIVOS Evaluar cuál es el riesgo de contraer una enfermedad infecciosa originada po...

Detección de la enfermedad meningocócica por reacción en cadena de la polimerasa

We submit the contribution of molecular techniques to the diagnosis of pediatric meningococcal disease from January 2001 to December 2005. Culture isolation and serogroup of Neisseria meningitidis was performed using traditional serology and biochemical methods. With regard to molecular techniques, we fi...

Prevalencia de la infección por Chlamydia trachomatis en mujeres de la ciudad de Bahía Blanca. Provincia de Buenos Aires, Argentina
Prevalence of infection by chlamydia trachomatis in women at Bahia Blanca city. Buenos Aires province, Argentina

Chlamydia trachomatis es considerada actualmente como uno de los agentes más comunes de enfermedades transmitidas sexualmente. En mujeres es causa de uretritis y cervicitis, y puede llevar a complicaciones, tales como enfermedad inflamatoria pélvica, embarazo ectópico e infertilidad tubaria. La transm...

Prevalencia de infección por virus de hepatitis G en una cohorte de pacientes hemofílicos HIV+
[Prevalence of hepatitis G virus infection in a cohort of hemophilic HIV positive patients]

Medicina (B.Aires); 62 (2), 2002
We analysed the prevalence of hepatitis G virus (HGV) infection in HCV+/HIV+ hemophilic patients determining HGV viremia in plasma by polymerase chain reaction (PCR). The overall prevalence of HGV infection was 13.51...

Relationship between diversity of hepatitis C quasispecies and histological severity of liver disease

Medicina (B.Aires); 60 (5/1), 2000
The aim of this work to assess in the diversity of hepatitis C virus (HCV) quasispecies is related to histological severity and duration of infection in a cohort of untreated patients with an estimated onset of the disease. A total of 27 patients with diagnosis of chronic liver disease and history of blo...

The detection and significance of minimal residual disease in chronic myeloid leukemia

Medicina (B.Aires); 60 Suppl 2 (), 2000
The focus of the study of minimal residual disease (MRD) is to redefine the concept of remission by using more sensitive molecular techniques to detect level of disease burden below that of conventional pathology. The detection of the chimeric bcr-abl mRNA transcript in chronic myeloid leukemia (CML) is ...

Development of monoclonal oligobodies and chemically synthesized oligobodies

Medicina (B.Aires); 60 Suppl 2 (), 2000
Oligonucleotide aptamers obtained using the SELEX procedure can recognize different molecules with high affinity. However, for proteins, this recognition is limited to native conformations and the specificity has not been clearly demonstrated by methods such as Western blotting, immunohistochemistry or i...