Rev. Soc. Bras. Med. Trop.(online); 48 (6), 2015
Ano de publicação: 2015
Introduction:
Human metapneumovirus (hMPV) is an emergent human respiratory pathogen. This study aimed to evaluate the performance of direct immunofl uorescence (DIF) to detect hMPV in a clinical laboratory setting. Methods:
Nasopharyngeal aspirate samples (448) of children and adults with respiratory illness were used to detect hMPV by using DIF and real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. Results:
In all, 36 (8%) samples were positive by DIF and 94 (21%) were positive by qRT-PCR. Direct immunofl uorescence specifi city was 99% and sensitivity was 38%.
Conclusions:
DIF is not very sensitive under clinical laboratory settings. (AU)