Results: 10

Desarrollo y evaluación de una base de datos in house para la identificación rápida de Burkholderia contaminons por EM MALDI-TOF

Rev. argent. microbiol; 51 (3), 2019
La espectrometría de masas (EM) (matrix assisted laser desorption ionization-time of flight) MALDI-TOF demostró ser una herramienta robusta para la identificación de numerosos grupos taxonómicos. No obstante, presenta limitaciones. Una ventaja clave de la técnica es la flexibilidad para la incorpora...

Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry

Braz. j. microbiol; 49 (4), 2018
ABSTRACT Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal protei...

Direct identification of bovine mastitis pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in pre-incubated milk

Braz. j. microbiol; 49 (4), 2018
ABSTRACT The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were s...

Concordance analysis between different methodologies used for identification of oral isolates of Candida species

Colomb. med; 49 (3), 2018
Abstract Background: The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia. Methods: Sixty...

Produção de biofilme na presença de agentes antimicrobianos e potencial de virulência para Caenorhabditis elegans de diferentes biótipos de Staphylococcus haemolyticus isolados de sangue
Biofilm production in the presence of antimicrobial agents and virulence potential for Caenorhabditis elegans from different strains of Staphylococcus haemolyticus isolated from blood

Os Staphylococcus coagulase-negativos (SCN) são encontrados na pele e mucosas de seres humanos e outros animais, já que algumas espécies são parte constituinte da microbiota normal destes mesmos sítios, e podem constituir um reservatório para SCN. Staphylococcus haemolyticus é a segunda espécie, ...

Displacers improve the selectivity of phosphopeptide enrichment by metal oxide affinity chromatography

Abstract: Background: A key process in cell regulation is protein phosphorylation, which is catalyzed by protein kinases and phosphatases. However, phosphoproteomics studies are difficult because of the complexity of protein phosphorylation and the number of phosphorylation sites. Methods: We describ...

Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry for identification of Clostridium species isolated from Saudi Arabia

Braz. j. microbiol; 47 (2), 2016
Abstract The aim of this study was to identify different Clostridium spp. isolated from currency notes from the Ha’il region of Saudi Arabia in September 2014 using MALDI–TOF-MS. Clostridium spp. were identified by Bruker MALDI–TOF-MS and compared with VITEK 2. The confirmation of the p...

Evaluación de la espectrometría de masas: MALDI-TOF MS para la identificación rápida y confiable de levaduras
Evaluation of mass spectrometry: MALDI-TOF MS for fast and reliable yeast identification

Rev. argent. microbiol; 47 (2), 2015
La espectrometría de masas, conocida como matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), es una técnica utilizada en la identificación de microorganismos mediante la creación de un espectro basado en el perfil de proteínas, que es único para una especie...

Metodología de pre-procesamiento de datos adquiridos por MALDI-MSI en muestras de tejidos
Pre-processing methodology of acquired data by MALDI-MSI in tissue samples

En la última década, la Imagenología por Espectrometría de Masas MALDI (MALDI-MSI) ha demostrado su potencial en el campo de la proteómica. Debido a la alta dimensionalidad de los conjuntos de datos obtenidos en los experimentos de MADI-MSI y las variabilidades inherentes al proceso de adquisición,...

Proteomic analysis of the regenerating liver following 2/3 partial hepatectomy in rats

Biol. Res; 47 (), 2014
BACKGROUND: Liver regeneration (LR) after 2/3 partial hepatectomy (PH) is one of the most studied models of cell, organ, and tissue regeneration. Although the transcriptional profile analysis of regenerating liver has been carried out by many reserachers, the dynamic protein expression profile during LR ...