Abstract The in vitro sporulation of Didymella bryoniae is of great importance for studies that require pure inoculum and in large quantities. Thus, the objectives of this study were to identify the best condition for D. bryoniae sporulation combining different light spectra (UV-A or UV-B light, white li...
The cocoa and palm oil agro-industries active in the state of Bahia, Brazil, generate high quantities of lignocellulosic wastes that could be recycled through their use in the formulation of substrates to cultivate edible mushrooms. Pleurotus ostreatus, also known as oyster mushroom, is the second most c...
Abstract The present study conducted a genetic characterization and determined growth rate and biomass production in solid and liquid media, using strains obtained from wild edible sporomes of Lyophyllum that grow in high mountains. Vegetative isolation was used to obtain a total of four strains, which w...
Agaricales/química,
Agaricales/genética,
Agaricales/crecimiento & desarrollo,
Agaricales/metabolismo,
Biomasa,
Medios de Cultivo/química,
Medios de Cultivo/metabolismo,
Fermentación,
Cinética,
México,
Micelio/química,
Micelio/genética,
Micelio/crecimiento & desarrollo,
Micelio/metabolismo
Se evaluaron 3 metodologías de extracción de proteínas para la identificación de hongos miceliales por MALDI-TOF MS en 44 aislados: la extracción con agua-ácido fórmico (E. agua), la extracción con zirconio-etanol-acetonitrilo-ácido fórmico (E. zirconio) y la recomendada por el proveedor del eq...
ABSTRACT Fungi of Pleurotus genus have attracted a great interest due to their medicinal properties such as anti-inflammatory, antimicrobial and antitumor. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. This work aimed to study the mycelial growth of P. ostreatus in s...
ABSTRACT Edible mushroom species are considered as an adequate source of food in a healthy diet due to high content of protein, fiber, vitamins, and a variety of minerals. The representatives of Pleurotus genus are characterized by distinct gastronomic, nutritional, and medicinal properties among the edi...
ABSTRACT Lectins are non-immunogenic carbohydrate-recognizing proteins that bind to glycoproteins, glycolipids, or polysaccharides with high affinity and exhibit remarkable ability to agglutinate erythrocytes and other cells. In the present study, ten Fusarium species previously not explored for lectins ...
ABSTRACT Dermatophytes are classified in three genera, Epidermophyton, Microsporum and Trichophyton. They have the capacity to invade keratinized tissue to produce a cutaneous infection known as dermatophytoses. This investigation was performed to study the effect of gaseous ozone and ozonized oil on thr...
Antifúngicos/farmacología,
Arthrodermataceae/efectos de los fármacos,
Ozono/farmacología,
Arthrodermataceae/fisiología,
Metabolismo de los Hidratos de Carbono/efectos de los fármacos,
Electrólitos/metabolismo,
Activación Enzimática,
Proteínas Fúngicas/metabolismo,
Micelio,
Permeabilidad,
Esporas Fúngicas/efectos de los fármacos
Abstract Metabolites of mycoparasitic fungal species such as Trichoderma harzianum 88 have important biological roles. In this study, two new ketoacyl synthase (KS) fragments were isolated from cultured Trichoderma harzianum 88 mycelia using degenerate primers and analysed using a phylogenetic tree. The ...
Proteínas Fúngicas/metabolismo,
Sintasas Poliquetidas/metabolismo,
Trichoderma/enzimología,
Secuencia de Aminoácidos,
Proteínas Fúngicas/química,
Proteínas Fúngicas/genética,
Regulación Fúngica de la Expresión Génica,
Datos de Secuencia Molecular,
Micelio/enzimología,
Micelio/genética,
Enfermedades de las Plantas/microbiología,
Sintasas Poliquetidas/química,
Sintasas Poliquetidas/genética,
Alineación de Secuencia,
Trichoderma/clasificación,
Trichoderma/genética
En este estudio se desarrolló y se evaluó el ensayo por inmunoabsorción ligado a enzimas (ELISA), para la detección de anticuerpos en sueros de pacientes con esporotricosis, para lo cual se empleó un antígeno crudo de Sporothrix schenckii sensu stricto obtenido a partir de la forma micelial. Los su...
Ensayo de Inmunoadsorción EnzimáticaMétodos,
Sporothrix/aislamiento & purificación,
Esporotricosis/diagnóstico,
Antígenos Fúngicos/inmunología,
ContrainmunoelectroforesisMétodos,
InmunodifusiónMétodos,
Micelio,
Sensibilidad y Especificidad,
Pruebas SerológicasMétodos,
Sporothrix/inmunología,
Esporotricosis/inmunología
