Abstract The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds. A total of 443 genomic DNA extracted from avian fecal samples were analyzed by one-tube nested real-time PCR and conve...
Abstract Leishmaniasis is a zoonotic disease caused by over 20 species of protozoan parasites of the genus Leishmania. Infection is commonly spread by sandflies and produces a wide spectrum of clinical signs and symptoms. Therefore, from an epidemiological and therapeutic standpoint, it is important to d...
Lymphoma is a neoplasm of hematopoietic origin that affects canines. The proper establishment of prognosis and rapid institution of treatment are essential for a better quality of life, and immunophenotyping is one of the tools
used for this purpose. The objective of this study was to perform a clonality...
Insulin-like growth factor-1 (IGF-1) is regarded as a crucial clinically significant therapeutic agent against several pathological conditions. Recently, recombinant DNA (rDNA) technology has enabled the production of many drugs of rDNA-origin including IGF-1. Securing a readily available supply of IGF-1...
The genus Mycoplasma includes more than 200 bacterial species that cause disease in animals. It is responsible for causing mastitis in bovines and may be related to other manifestations, such as arthritis and pneumonia in calves and heifers. The present study aimed to detect Mycoplasma bovis isolated fro...
Dias, Renata Cristina Ferreira;
Pasquali, Aline Kuhn Sbruzzi;
Thomaz-Soccol, Vanete;
Pozzolo, Eliane Maria;
Chiyo, Luciana;
Alban, Silvana Maria;
Fendrich, Ricardo Cancio;
Almeida, Rômulo Augusto Andrade de;
Ferreira, Fernanda Pinto;
Caldart, Eloiza Teles;
Freire, Roberta Lemos;
Mitsuka-Breganó, Regina;
Bisetto Júnior, Alceu;
Navarro, Italmar Teodorico.
Abstract The aim of this study was to confirm the emergence of canine visceral leishmaniasis among dogs in Foz do Iguaçu. The disease was diagnosed through the isolation and molecular identification of Leishmania infantum. In the first sample collection stage (2012), three lymph node aspirates and 46 bu...
Brasil/epidemiología,
ADN Protozoario/genética,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Enfermedades de los Perros/parasitología,
Leishmania infantum/genética,
Leishmania infantum/aislamiento & purificación,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria
The aim was to determine the spread of genetically similar profiles of Campylobacter in chicken carcasses and evaluate their ability to produce transcripts for ciaB, dnaJ, p19 and sodB genes, before and after cultivation in Caco-2 cells. The strains used were isolated from 420 samples of chicken carcasse...
Bacteria of the genus Brachyspira can cause enteric diseases in poultry causing a decrease in productivity. The occurrence of this disease in chickens has already been verified in countries such as Australia, Italy, and the United States, but in Brazil, until now, epidemiological studies about Brachyspir...
Fernandes, Murilo Antônio;
Leonel, João Augusto Franco;
Isaac, Jéssica Anzolin;
Benassi, Julia Cristina;
Silva, Diogo Tiago;
Spada, Julio Cesar Pereira;
Pereira, Nuno Wolfgang Balbini;
Ferreira, Helena Lage;
Keid, Lara Borges;
Soares, Rodrigo Martins;
Oliveira, Trícia Maria Ferreira de Sousa.
Abstract The aim of this study was to compare molecular tests used to diagnose Leishmania spp. in dogs with different stages of infection. Blood and conjunctival swab (CS) samples from dogs classified in four clinical stages were subjected to different PCR protocols (13A/13B, MC1/MC2, LITSR/L5.8S and LEI...
Brasil/epidemiología,
ADN Protozoario/genética,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Leishmania infantum/genética,
Leishmaniasis Cutánea/diagnóstico,
Leishmaniasis Cutánea/epidemiología,
Leishmaniasis Cutánea/veterinaria,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria,
Índice de Severidad de la Enfermedad
Viral hemorrhagic diseases in cervids occur worldwide and include epizootic hemorrhagic disease (EHD), bluetongue (BT), and adenoviral hemorrhagic disease (AHD). Since gross lesions in all three hemorrhagic diseases are identical (hemorrhagic enteropathy, pulmonary edema, systemic petechial and suffusion...
