Results: 30

Optimizing factors for the efficient expression and purification of spao and lamb from Salmonella typhi

Biosci. j. (Online); 38 (), 2022
We optimized the expression and purification of outer membrane proteins SpaO and LamB from Salmonella typhi. We investigated various factors in the expression and purification processes, including the use of isopropyl β-d-1 thiogalactopyranoside (IPTG), imidazole, and urea. First, PCR amplification was ...

Biological activity of synthesized 5-{1-[(4-chlorophenyl)sulfonyl]piperidin-4-yl}-2-mercapto-1, 3, 4-oxadiazole derivatives demonstrated by in silico and BSA binding studies

We synthesized a series of compounds bearing pharmacologically important 1,3,4-oxadiazole and piperidine moieties. Spectral data analysis by 1H-NMR, 13C-NMR, IR and EI-MS was used to elucidate the structures of the synthesized molecules. Docking studies explained the different types of interaction of the...

Absceso cerebral causado por salmonella typhi: reporte de caso
Brain abscess caused by salmonella typhi: case report

Rev. med. interna Guatem; 21 (3), 2017
Se presenta el caso de un paciente de 50 años de edad, quien es admitido a la emergencia de adultos, con cuadro un convulsivo asociado a fiebre de una semana de evolución. El estudio de tomografía cerebral reveló la presencia de imagen heterogénea en lóbulo frontal , se interviene quirúrgicamente ...

Determinación rápida de la identidad del polisacárido de Salmonella Typhi y toxoide diftérico en vacunas conjugadas
Rapid determination of the identity of Salmonella Typhi polysaccharide and diphtheria toxoid in conjugate vaccines

El polisacárido Vi (PsVi) de Salmonella Typhi es un antígeno T-independiente y ha demostrado ser protector en adultos jóvenes. Sin embargo, para aumentar la respuesta de anticuerpos y conferir propiedades T-dependientes al polisacárido, se ha conjugado a proteínas. Dentro de los controles exigidos p...

Differential roles for pathogenicity islands SPI-13 and SPI-8 in the interaction of Salmonella Enteritidis and Salmonella Typhi with murine and human macrophages

Biol. Res; 50 (), 2017
BACKGROUND: Salmonella pathogenicity island (SPI)-13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Typhimurium and S. Gallinarum. However, it is absent in typhoid serovars such as S. Typhi and Paratyphi A, which carry SPI-8 at the same genomic location. Because the interactio...

stg fimbrial operon from S. Typhi STH2370 contributes to association and cell disruption of epithelial and macrophage-like cells

Biol. Res; 48 (), 2015
BACKGROUND: Salmonella enterica serovar Typhi (S. Typhi) stg operon, encoding a chaperone/usher fimbria (CU), contributes to an increased adherence to human epithelial cells. However, one report suggests that the presence of the Stg fimbria impairs the monocyte-bacteria association, as deduced by the low...

In vivo antipyretic, antiemetic, in vitro membrane stabilization, antimicrobial, and cytotoxic activities of different extracts from Spilanthes paniculata leaves

Biol. Res; 47 (), 2014
BACKGROUND: The study was conducted to evaluate the in vitro antimicrobial activity, cytotoxic, and membrane stabilization activities, and in vivo antiemetic and antipyretic potentials of ethanolic extract, n-hexane and ethyl acetate soluble fractions of Spilanthes paniculata leaves for the first time wi...

Evaluacion de la actividad insecticida in vitro sobre larvas de Aedes aegypti (vector de la fiebre amarilla y dengue) y determinación del efecto antibacteriano frente a cepas de Salmonella typhi spp. de las acetogeninas obtenidas a partir de extractos etanólicos de semillas de Annona muricata L. (masasamba)
Evaluation of in vitro insecticidal activity on Aedes aegypti larvae (vector of yellow fever and dengue) and determination of the antibacterial effect against strains of Salmonella typhi spp. of acetogenins obtained from ethanolic extracts of seeds of Annona muricata L. (masasamba)

El presente trabajo evaluó el efecto insecticida y efecto antibacteriano de las Acetogeninas extraídas a partir de extractos etanólicos de semillas de Annona muricata L. (Masasamba), el desarrollo de la investigación fue llevado a cabo en diferentes escenarios de la región Cusca (provincias de la Co...

Antibacterial activity of Apis mellifera L. propolis collected in three regions of Kenya

The present study aimed at investigating the susceptibility of the microorganisms Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis to ethanolic extracts of propolis (EEP) from three regions of Kenya (Taita, Tana and Samburu). Propolis was extracted ...

Hematoquezia maciça em paciente com febre tifóide: o que fazer?: relato de caso e revisão da literatura
Massive hematoquezia in patient with typhoid fever: what to do?: case related and literature revision

J. bras. med; 91 (5/6), 2006
A febre tifóide continua sendo um problema de saúde pública em países com precárias condições sanitárias, representando ainda um desafio o seu diagnóstico precoce, tratamento e prevenção. Este trabalho relata um caso de febre tifóide com complicações hemorrágicas intestinais graves, tratad...