BACKGROUND: Chinese hamster ovary cell line has been used routinely as a bioproduction factory of numerous biopharmaceuticals. So far, various engineering strategies have been recruited to improve the production efficiency of this cell line such as apoptosis engineering. Previously, it is reported that t...
BACKGROUND: Cisplatin resistance (DDP-resistance) remains one of the major causes of poor prognosis in females with ovarian cancer. Long non-coding RNAs (lncRNAs) have been shown to participate in the regulation of cellular processes, including chemoresistance. The aim of this study was to explore the ro...
Cisplatino/farmacología,
Resistencia a Antineoplásicos/genética,
Neoplasias Ováricas/genética,
ARN Largo no Codificante/genética,
Apoptosis/efectos de los fármacos,
Proteína Potenciadora del Homólogo Zeste 2/antagonistas & inhibidores,
Regulación Neoplásica de la Expresión Génica/efectos de los fármacos,
Técnicas de Inactivación de Genes/métodos,
MicroARNs/antagonistas & inhibidores,
Sirtuina 1/antagonistas & inhibidores,
Línea Celular Tumoral,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Regulación hacia Arriba
Abstract Objective: To investigate the relation between biofilm formation ability and quorum sensing gene LuxS/AI-2. Materials and Methods: Enterococcus faecalis (E. faecalis) standard strain ATCC 29212 was used in the study. Long flanking homology polymerase chain reaction method was used to build the...
Análisis de Varianza,
Proteínas Bacterianas/genética,
Proteínas Bacterianas/fisiología,
Biopelículas/crecimiento & desarrollo,
Liasas de Carbono-Azufre/genética,
Liasas de Carbono-Azufre/fisiología,
Recuento de Colonia Microbiana,
Enterococcus faecalis/genética,
Enterococcus faecalis/crecimiento & desarrollo,
Técnicas de Inactivación de Genes,
Microscopía Confocal,
Microscopía Electrónica de Rastreo,
Plásmidos,
Percepción de Quorum/genética,
Percepción de Quorum/fisiología,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Factores de Tiempo
Background: Zymomonas mobilis is a Gram-negative microaerophilic bacterium with excellent ethanol-producing capabilities. The RecET recombination system provides an efficient tool for direct targeting of genes in the bacterial chromosome by PCR fragments. Results: The plasmids pSUZM2a-RecET and pSUZM2a-R...
Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of ...
Summary Objective: Dermal papilla cells (DPCs) are located in the hair follicles and play an important role in hair growth. These cells have the ability to induce hair follicle formation when they display aggregative behavior. DPCs derived from the androgenetic alopecia (AGA) area undergo premature sene...
Alopecia/genética,
Agregación Celular/genética,
Ciclo Celular/genética,
Proliferación Celular/genética,
Células Cultivadas,
Senescencia Celular/genética,
Dermis/citología,
Citometría de Flujo,
Técnicas de Inactivación de Genes/métodos,
Genes p16/fisiología,
Folículo Piloso/citología,
Inmunohistoquímica,
Valores de Referencia,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Cuero Cabelludo/citología,
Factores de Tiempo,
Transfección
Yu, Yi;
Chang, De;
Xu, Huiwen;
Zhang, Xuelin;
Pan, Lei;
Xu, Chou;
Huang, Bing;
Zhou, Hong;
Li, Jia;
Guo, Jun;
Liu, Changting.
Abstract Streptococcus pneumoniae is one of the most frequent opportunistic pathogens worldwide. DNA processing protein A (DprA) is an important factor involved in bacterial uptake and DNA integration into bacterial genome, but its role in S. pneumoniae virulence remains unclear. The aim of this study wa...
Adhesión Bacteriana,
Proteínas Bacterianas/metabolismo,
Proteínas de la Membrana/metabolismo,
Infecciones Neumocócicas/patología,
Streptococcus pneumoniae/patogenicidad,
Factores de Virulencia/análisis,
Proteínas Bacterianas/análisis,
Proteínas Bacterianas/genética,
Línea Celular,
Modelos Animales de Enfermedad,
Células Epiteliales/microbiología,
Técnicas de Inactivación de Genes,
Ratones,
Infecciones Neumocócicas/microbiología,
Streptococcus pneumoniae/genética,
Análisis de Supervivencia,
Factores de Virulencia/genética,
Proteínas de la Membrana/genética
BACKGROUND Tuberculosis (TB) is an infectious disease caused mainly by the bacillus Mycobacterium tuberculosis. The better understanding of important metabolic pathways from M. tuberculosis can contribute to the development of novel therapeutic and prophylactic strategies to combat TB. Nucleoside hydrol...
RIC-8B é um fator trocador de nucleotídeo de guanina (GEF) predominantemente expresso em neurônios olfatórios maduros de camundongos adultos. Trabalhos desenvolvidos em nosso laboratório mostraram que RIC-8B interage com Gαolf e Gγ13, duas subunidades de proteína G que estão enriquecidas nos cí...
RIC-8B é uma proteína que apresenta, in vitro, atividade de fator de troca de nucleotídeos guanina (GEF). No entanto, seu papel in vivo não é conhecido. Dados anteriores do nosso laboratório demonstraram que essa proteína interage especificamente com Gαolf, que é uma proteína G exclusiva do sis...
