Sobre la vacuna rusa Sputnik V y su eficacia
Revista Digital de Postgrado; 10 (2), 2021
Results: 22
Lentivirus vectors fail to deliver transgenes into bovine zygotes after co-incubation with sperm during in vitro fertilization
Arq. bras. med. vet. zootec. (Online); 73 (1), 2021
As vantagens dos animais transgênicos têm sido demonstradas em diferentes aplicações, entretanto muitas metodologias usadas para gerar animais geneticamente modificados (GM) apresentam baixas taxas de eficiência. O objetivo deste estudo foi avaliar a entrega dos vetores lentivirais (VLs) em zigotos ...
Characterisation of alternative expression vectors for recombinant Bacillus Calmette-Guérin as live bacterial delivery systems
Mem. Inst. Oswaldo Cruz; 115 (), 2020
BACKGROUND Bacillus Calmette-Guérin (BCG) is considered a promising live bacterial delivery system. However, several proposals for rBCG vaccines have not progressed, mainly due to the limitations of the available expression systems. OBJECTIVES To obtain a set of mycobacterial vectors using a range of...
In-Situ Gel-Free Plasmid Reassembling for Rapid Gene Subcloning and Truncation
Braz. arch. biol. technol; 63 (), 2020
Abstract Gene subcloning, a process in which the nucleotide sequence of interest is excised from on plasmid and inserted into another, seems to be an easy task to done. However, not all subcloning attempts are successful, even when the insert sequence and the double digested target plasmid are successful...
Relationship between PI3K/mTOR/RhoA pathway-regulated cytoskeletal rearrangements and phagocytic capacity of macrophages
Braz. j. med. biol. res; 53 (7), 2020
The objective of this study was to investigate the relationship between PI3K/mTOR/RhoA signaling regulated cytoskeletal rearrangements and phagocytic capacity of macrophages. RAW264.7 macrophages were divided into four groups; blank control, negative control, PI3K-RNAi, and mTOR-RNAi. The cytoskeletal ch...
Western Blotting, Citoesqueleto/metabolismo, Silenciador del Gen, Vectores Genéticos, Macrófagos/metabolismo, Fagocitosis/fisiología, Fosfatidilinositol 3-Quinasas/metabolismo, Células RAW 264.7, Interferencia de ARN, Reacción en Cadena en Tiempo Real de la Polimerasa, Transducción de Señal, Serina-Treonina Quinasas TOR/metabolismo, Transfección, Proteína de Unión al GTP rhoA/metabolismo
Respuesta inmune y terapia gó©nica con vectores virales adenoasociados
Immune response and gene therapy with adenoassociated viral vectors
Medicina (B.Aires); 79 (6), 2019
En los ó;ºltimos aó;±os la terapia gó;©nica se ha posicionado como una opció;n real y segura en el desarrollo de alternativas terapó;©uticas para la cura y la prevenció;n de diferentes enfermedades. Consiste en la inserció;n de material genó;©tico en un tejido o có;©lula defectuosa, median...
Methods for the genetic manipulation of marine bacteria
Electron. j. biotechnol; 33 (), 2018
Genetic manipulation of bacteria is a procedure necessary to obtain new strains that express peculiar and defined genetic determinants or to introduce genetic variants responsible for phenotypic modifications. This procedure can be applied to explore the biotechnological potential associated with environ...
Improving adenoviral vectors and strategies for prostate cancer gene therapy
Clinics; 73 (supl.1), 2018
Gene therapy has been evaluated for the treatment of prostate cancer and includes the application of adenoviral vectors encoding a suicide gene or oncolytic adenoviruses that may be armed with a functional transgene. In parallel, versions of adenoviral vector expressing the p53 gene (Ad-p53) have been te...
Adenoviridae/genética, Carcinoma de Pulmón de Células no Pequeñas/genética, Genes Transgénicos Suicidas, Terapia Genética/métodos, Vectores Genéticos/uso terapéutico, Neoplasias Pulmonares/genética, Proteínas de Neoplasias/genética, Antígeno Prostático Específico/genética, Neoplasias de la Próstata/genética, Neoplasias de la Próstata/inmunología, Neoplasias de la Próstata/terapia, Proteína p53 Supresora de Tumor/biosíntesis
The effect of downregulation of Stathmin gene on biological behaviors of U373 and U87-MG glioblastoma cells
Biol. Res; 51 (), 2018
BACKGROUND: Stathmin as a critical protein involved in microtubule polymerization, is necessary for survival of cancer cells. However, extremely little is known about Stathmin in glioblastoma. So, this study was designed to elucidate the function of Stathmin gene in the tumorigenesis and progression of g...
Development of a novel vector for cloning and expressing extremely toxic genes in Escherichia coli
Electron. j. biotechnol; 30 (), 2017
Background: Escherichia coli has been widely used as a host to clone and express heterologous genes. However, there are few vectors available for cloning and expressing extremely toxic genes, which limits further basic and applied research on extremely toxic proteins. Results: In this study, a novel vect...
Escherichia coli/genética, Proteínas de Escherichia coli/toxicidad, Vectores Genéticos, Triptófano/metabolismo, Regiones Promotoras Genéticas, Proteínas Co-Represoras, Genes Bacterianos, ARN sin Sentido, Isopropil Tiogalactósido/metabolismo, Reacción en Cadena de la Polimerasa, Electroforesis en Gel de Poliacrilamida, Desoxirribonucleasa BamHI/metabolismo, Western Blotting, Clonación Molecular