Results: 10

Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA

Braz. j. microbiol; 49 (1), 2018
ABSTRACT We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity...

Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations

Braz. j. infect. dis; 21 (6), 2017
ABSTRACT Objectives: The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is ind...

Seroprevalence and spatial distribution dynamics of Yersinia pestis antibodies in dogs and cats from plague foci in the State of Ceará, Northeastern Brazil

Abstract INTRODUCTION: In Brazil, the plague is established in several foci located mainly in the northeastern part of the country, where it alternates between active and quiescent periods. These foci in the State of Ceará have high epidemiological importance. In addition to other plague detection act...

Purificación y control de calidad de la fracción antigénica F1 de yersinia pestis
Purification and quality control of Yersinia pestis F1 antigenical fraction

Rev. med. exp; 16 (1/2), 1999
Se ha desarrollado la extracción y purificación de la fracción de antigénica F1 de Yersinia pestis que se utilizará en la producción de un kit para el diasgnóstico de peste. El proceso se realizó a partir de biomasa de una cepa patógena de Yersinia pestis, aislada en Chiclayo (1999), cuyos facto...

Manual de procedimientos de laboratorio para el diagnóstico de peste
Manual of laboratory procedures for the diagnosis pest

Este documento detalla: Presentación, resolución jefatural, introducción, obtención y envío de la muestra, pruebas de laboratorio para diagnóstico de peste, inmunofluorescencia directa (IFA), prueba de aglutinación en látex, aislamiento por cultivo de Yersinia pestis, técnicas de ELISA, inoculac...

Pesquisa de Yersinia pestis em roedores e outros pequenos mamíferos nos focos pestosos do nordeste do Brasil no período 1966 a 1982
Detection of Yersinia pestis in rodents and other small mammals in the northeast of Brazil during the period from 1966 to 1982

Rev. saúde pública; 21 (3), 1987
Foi feita análise da metodologia empregada e dos resultados alcançados em pesquisa de Yersinia pestis, em material de 24.703 roedores e outros pequenos mamíferos oriundos dos focos pestosos do Nordeste do Brasil, no período de 1966 a 1982. Concluiu-se ser necessário haver maior rapidez na realizaç...

Informe sobre a peste bubonica (infeccao por Yersinia pestis) no Estado de Minas Gerais, Brasil.
Report on bubonic plague (Yersinia pestis infection) in Minas Gerais State, Brazil

Rev. microbiol; 15 (3), 1984
Yersinia pestis foi isolada de roedores e detectados anticorpos antipestosos, em soros humanos e de roedor, do Estado de Minas Gerais, onde a infeccao pestosa nao era registrada, ha cerca de 10 anos...