The consumption of soybean isoflavones (IS) is associated with several beneficial properties on human health. Some lactic acid bacteria possess ß-glucosidase enzyme, that allows to obtain the active form of IS (aglycone). The solid state fermentation (SSF) has received great attention in the last years ...
Fermentación,
Isoflavonas/biosíntesis,
Lacticaseibacillus rhamnosus/metabolismo,
Glycine max/metabolismo,
Productos Vegetales/análisis,
Aminoácidos/metabolismo,
Proteínas Bacterianas/metabolismo,
Microbiología de Alimentos,
Hidrólisis,
Ácido Láctico/biosíntesis,
Sacarosa/farmacología,
beta-Glucosidasa/metabolismo
Abstract The cellulolytic activity of fungi growing in the subtropical rainforest of Misiones (Argentina) represents a challenge in the technological development of the production of cellulosic bioethanol in the region using native sources. These fungi are promising to obtain sustainable enzyme cocktails...
Background: Cellulolytic enzymes of microbial origin have great industrial importance because of their wide application in various industrial sectors. Fungi are considered the most efficient producers of these enzymes. Bioprospecting survey to identify fungal sources of biomass-hydrolyzing enzymes from a...
Penicillium/enzimología,
beta-Glucosidasa/biosíntesis,
Hidrólisis,
Celulasa/biosíntesis,
Lignina/metabolismo,
Trichoderma/enzimología,
Biocatálisis,
Fermentación,
Oligosacáridos,
Ecosistema Amazónico,
Concentración de Iones de Hidrógeno,
Temperatura,
Estabilidad de Enzimas,
beta-Glucosidasa/metabolismo,
Celulasa/metabolismo
Background: β-Glucosidase assay is performed with purified or semipurified enzymes extracted from cell lysis. However, in screening studies, to find bacteria with β-glucosidase activity among many tested bacteria, a fast method without cell lysis is desirable. In that objective, we report an in vivo β...
Bifidobacterium/aislamiento & purificación,
Bifidobacterium/enzimología,
Bifidobacterium longum/aislamiento & purificación,
Bifidobacterium longum/enzimología,
Bifidobacterium pseudocatenulatum/aislamiento & purificación,
Bifidobacterium pseudocatenulatum/enzimología,
beta-Glucosidasa/metabolismo,
Pruebas de Enzimas,
Nitrofenilgalactósidos,
Nitrofenoles,
Lactobacillus/enzimología,
Lactobacillus/aislamiento & purificación,
Bifidobacterium/metabolismo,
Lactobacillus/metabolismo
Background: Ginsenoside is the most important secondary metabolite in ginseng. Natural sources of wild ginseng have been overexploited. Although root culture can reduce the length of the growth cycle of ginseng, the number of species of ginsenosides is reduced and their contents are lower in the adventit...
Panax/metabolismo,
Raíces de Plantas/metabolismo,
Ginsenósidos/metabolismo,
Panax/crecimiento & desarrollo,
beta-Glucosidasa/metabolismo,
Reactores Biológicos,
Fermentación,
Ginsenósidos/aislamiento & purificación,
Panax/química,
Raíces de Plantas/química,
Cromatografía Líquida de Alta Presión,
Penicillium,
Biotransformación
Background β-Glucosidases catalyze the hydrolysis of cellobiose and cellodextrins, releasing glucose as the main product. This enzyme is used in the food, pharmaceutical, and biofuel industries. The aim of this work is to improve the β-glucosidase production by the fungus Lichtheimia ramosa by ...
Background: β-Glucosidase is known as an effective catalyst for the hydrolysis of various glycosides and immobilization is one of the most efficient strategies to improve its activity recovery and properties. Results: Crosslinking-adsorption-crosslinking method was employed to immobilize β-gluc...
La Enfermedad de Gaucher es la enfermedad más común de depósito lisosomal, pertenece al grupo de enfermedades catalogadas como errores innatos del metabolismo y se trasmite por herencia autosómica recesiva. (1) Se caracteriza por la presencia de mutaciones patológicas en los genes que codifican la e...
Foram purificadas através de uma combinação de cromatografias as duas ß-glicosidases digestivas (Mr 47.000 e 50.000 - denominadas ßgli47 e ßgli50, respectivamente) encontradas na larva de S. frugiperda. Experimentos de competição entre substratos e modificação química mostraram que a ßgli47 p...
El microorganismo gram negativo que se aisla con mayor frecuencia en muestras clínicas es escherichia coli. Con el objeto de identificar en forma rápida la presencia de escherichia coli en los urocultivos, se ensayó un sistema de identificación acortado que incluyó prueba de oxidasa, indol y activid...
