Background: Antithrombin III (ATIII) is a protein that inhibits abnormal blood clots (or coagulation) by breaking down thrombin and factor Xa. ATIII helps to keep a healthy balance between hemorrhage and coagulation. The present work demonstrated the production, purification and characterization of recom...
Abstract Clostridium difficile has emerged as an increasingly important nosocomial pathogen and the prime causative agent of antibiotic-associated diarrhoea and pseudomembranous colitis in humans. In addition to toxins A and B, immunological studies using antisera from patients infected with C. difficile...
Anticuerpos Antibacterianos/análisis,
Proteínas Bacterianas/análisis,
Infecciones por Clostridium/microbiología,
Clostridioides difficile/genética,
Anticuerpos Antibacterianos/genética,
Anticuerpos Antibacterianos/inmunología,
Proteínas Bacterianas/genética,
Proteínas Bacterianas/inmunología,
Western Blotting,
Infecciones por Clostridium/diagnóstico,
Clostridioides difficile/inmunología,
Clostridioides difficile/aislamiento & purificación,
Expresión Génica,
Proteínas Recombinantes/análisis,
Proteínas Recombinantes/genética,
Proteínas Recombinantes/inmunología,
Ribotipificación
Background: Human is an essential cellular enzyme that is found in all human cells. As this enzyme is upregulated in cancer cells exceedingly, it is used as a target for cancer chemotherapeutic drug development. As such, producing the in-house enzyme for the purpose to speed up the search for more cost-e...
ABSTRACT Background The effectiveness of antiviral therapy with pegylated interferon and ribavirin for chronic hepatitis C is far from ideal and presents several adverse events. Among such events, there is the depressive episode that can even lead to treatment discontinuity Objective Analyze the incide...
Antivirales/efectos adversos,
Antivirales/uso terapéutico,
Estudios de Cohortes,
Depresión/inducido químicamente,
Depresión/psicología,
Quimioterapia Combinada,
Hepatitis C Crónica/tratamiento farmacológico,
Hepatitis C Crónica/psicología,
Interferón alfa-2,
Interferón-alfa/efectos adversos,
Interferón-alfa/uso terapéutico,
Persona de Mediana Edad,
Polietilenglicoles/efectos adversos,
Polietilenglicoles/uso terapéutico,
Estudios Prospectivos,
Proteínas Recombinantes/efectos adversos,
Proteínas Recombinantes/uso terapéutico,
Ribavirina/efectos adversos,
Ribavirina/uso terapéutico,
Factores Sociológicos
CONTEXT: Trichosanthin produced in the root tube of Trichosanthes kirilowii shows anti-tumor activity on a series of cancer cells including Hela, MCF-7, HL-60. But there is little information about its effect on the carcinogenesis of prostate cancer. OBJECTIVE: This work was designed to study the role of...
Antineoplásicos Fitogénicos/farmacología,
Neoplasias de la Próstata/tratamiento farmacológico,
Tricosantina/farmacología,
Apoptosis/efectos de los fármacos,
Proliferación Celular/efectos de los fármacos,
Neoplasias de la Próstata/patología,
Proteínas Recombinantes/farmacología,
Western Blotting,
Línea Celular Tumoral,
Formazáns,
Reproducibilidad de los Resultados,
Sales de Tetrazolio,
Factores de Tiempo,
Carga Tumoral
This study aimed to express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain; test this protein as an antigen in serological assays; and investigate its antigenicity and immunogenicity. A protein coded by an allele of the A2 gene isolated from L. chagasi was expressed in three di...
Antígenos de Protozoos/sangre,
Enfermedades de los Perros/sangre,
Enfermedades de los Perros/diagnóstico,
Leishmania infantum/inmunología,
Leishmania infantum/metabolismo,
Leishmaniasis Visceral/sangre,
Leishmaniasis Visceral/veterinaria,
Proteínas Protozoarias/biosíntesis,
Proteínas Protozoarias/sangre,
Proteínas Recombinantes/biosíntesis,
Proteínas Recombinantes/análisis,
Pruebas Serológicas
Background The fermentation conditions of recombinant maltose-binding protein fused to neutrophil-activating protein (rMBP-NAP) of Helicobacter pylori were optimized from Escherichia coli TB1 with varying medium, inoculum age and size, time, inducer, pH and temperature in batch fermentation. Results It w...
Liu, Zixuan;
Zhang, Jizhou;
Fan, Hongqiong;
Yin, Ruofeng;
Zheng, Zhong;
Xu, Qian;
Liu, Qing;
He, Haiting;
Peng, Xiaofan;
Wang, XinXin;
Li, Xiaokun;
Xiao, Yechen.
Background Overexpression or mutated activation of Fibroblast growth factor receptor 3 (FGFR3) is involved in the pathogenesis of many tumors. More and more studies focus on the potential usage of therapeutic antibodies against FGFR3. Results In this study, a novel single-chain Fv (ScFv) against FGFR3 wa...
A reabsorção do osso alveolar ocorre após a perda dentária por vários fatores, tais como: exodontia, traumatismo, processos patológicos ou mesmo reabsorção por desuso. A reabilitação com implantes requer osso em quantidade e qualidade. Os enxertos autógenos são considerados os enxertos tipo "...
Background Bacillus subtilis UMC7 isolated from the gut of termite Macrotermes malaccensis has the ability to secrete a significant amount of extracellular endoglucanase, with an enzyme activity of 0.12 ± 0.01 μmol/min/mL. However, for economically viable industrial applications, the enzyme needs t...
Bacillus subtilis/enzimología,
Bacillus subtilis/aislamiento & purificación,
Intestinos/microbiología,
Isópteros,
Celulasa/metabolismo,
Celulasa/genética,
Clonación Molecular,
Escherichia coli,
Análisis de Secuencia,
Amplificación de Genes,
Proteínas Recombinantes,
Temperatura,
Concentración de Iones de Hidrógeno,
Especificidad por Sustrato,
Iones,
Metales
