ABSTRACT Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has bee...
Abstract: Background: Mitochondriopathies are multisystem diseases affecting the oxidative phosphorylation (OXPHOS) system. Skin fibroblasts are a good model for the study of these diseases. Fibroblasts with a complex IV mitochondriopathy were used to determine the molecular mechanism and the main affe...
ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the a...
Smid, Jerusa;
Studart Neto, Adalberto;
Landemberger, Michele Christine;
Machado, Cleiton Fagundes;
Nóbrega, Paulo Ribeiro;
Canedo, Nathalie Henriques Silva;
Schultz, Rodrigo Rizek;
Naslavsky, Michel Satya;
Rosemberg, Sérgio;
Kok, Fernando;
Chimelli, Leila;
Martins, Vilma Regina;
Nitrini, Ricardo.
ABSTRACT Gerstmann-Sträussler-Scheinker is a genetic prion disease and the most common mutation is p.Pro102Leu. We report clinical, molecular and neuropathological data of seven individuals, belonging to two unrelated Brazilian kindreds, carrying the p.Pro102Leu. Marked differences among patients were o...
The aim of this study was to evaluate the extraction of dental DNA exposed to different chemical solutions. The sample was composed by 15 subjects, from which 5 samples of oral mucosal cells (reference population) and 15 teeth (experimental population) were collected. The experimental population was divi...
La necesidad de ampliar los conocimientos respecto a los mecanismos bioquímicos y fisiológicos desarrollados por los microorganismos presentes en suelos requiere de una descripción completa de la diversidad microbiana, para lo cual en las últimas décadas se han desarrollado diferentes técnicas mole...
Abstract Saliva when compared to blood collection has the following advantages: it requires no specialized personnel for collection, allows for remote collection by the patient, is painless, well accepted by participants, has decreased risks of disease transmission, does not clot, can be frozen before DN...
ADN/aislamiento & purificación,
Electroforesis,
Reacción en Cadena de la Polimerasa,
Control de Calidad,
Juego de Reactivos para Diagnóstico,
Valores de Referencia,
Reproducibilidad de los Resultados,
Saliva/química,
Manejo de Especímenes/métodos,
Espectrofotometría,
Estadísticas no Paramétricas,
Factores de Tiempo
Chromosomal instability, leading to aneuploidy, is one of the hallmarks of human cancers. USP44 (ubiquitin specific peptidase 44) is an important molecule that plays a regulatory role in the mitotic checkpoint and USP44 loss causes chromosome mis-segregation, aneuploidy and tumorigenesis in vivo. In this...
Kanegae, Marilia Pyles P;
Barreiros, Lucila Akune;
Sousa, Jusley Lira;
Brito, Marco Antônio S;
Oliveira Junior, Edgar Borges de;
Soares, Lara Pereira;
Mazzucchelli, Juliana Themudo L;
Fernandes, Débora Quiorato;
Hadachi, Sonia Marchezi;
Holanda, Silvia Maia;
Guimarães, Flavia Alice T. M;
Boacnin, Maura Aparecida P. V. V;
Pereira, Marley Aparecida L;
Bueno, Joaquina Maria C;
Grumach, Anete Sevciovic;
Gesu, Regina Sumiko W. Di;
Santos, Amélia Miyashiro N. dos;
Bellesi, Newton;
Costa-Carvalho, Beatriz T;
Condino-Neto, Antonio.
RESUMO Objetivo: Validar a quantificação de T-cell receptor excision circles (TRECs) e kappa-deleting recombination circles (KRECs) por reação em cadeia de polimerase (polymerase chain reaction, PCR) em tempo real (qRT-PCR), para triagem neonatal de imunodeficiências primárias que cursam com defe...
BACKGROUND: Cancer cells exhibit elevated levels of glucose uptake and may obtain pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid growth; they may also use nucleic acid from their microenvironment. The study of processing nucleic acid by cancer cells will help improve the u...
ADN/metabolismo,
Neoplasias/genética,
Transfección,
Neoplasias de la Mama/genética,
Neoplasias de la Mama/patología,
Endocitosis/genética,
Hepatocitos/metabolismo,
Lípidos/farmacología,
Neoplasias Hepáticas/genética,
Neoplasias Hepáticas/patología,
Neoplasias Pulmonares/genética,
Neoplasias Pulmonares/patología,
Neoplasias/patología,
alfa-Fetoproteínas/metabolismo,
Línea Celular,
Línea Celular Tumoral,
Fragmentación del ADN,
Genómica,
Hibridación Fluorescente in Situ,
Reacción en Cadena de la Polimerasa
