BACKGROUND A number of Zika virus (ZIKV) sequences were obtained using Next-generation sequencing (NGS), a methodology widely applied in genetic diversity studies and virome discovery. However Sanger method is still a robust, affordable, rapid and specific tool to obtain valuable sequences. OBJECTIVE ...
OBJECTIVES: Several compounds characterized by an olefin linkage conjugated to a carbonyl group have anti-inflammatory properties. The diuretic ethacrynic acid (EA) is a compound of this type. Herein, we tested the hypothesis that ethacrynic acid can modulate the development of ileus after bowel manipula...
Modelos Animales de Enfermedad,
Ácido Etacrínico/farmacología,
Tránsito Gastrointestinal/efectos de los fármacos,
Ileus/patología,
Ileus/cirugía,
Mediadores de Inflamación/antagonistas & inhibidores,
Interleucina-6/antagonistas & inhibidores,
Intestino Delgado/efectos de los fármacos,
Intestino Delgado/patología,
Ratones Endogámicos C57BL,
Óxido Nítrico Sintasa de Tipo II/antagonistas & inhibidores,
Complicaciones Posoperatorias,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Previous studies have indicated that propofol has immunomodulatory and antioxidative properties. However, the renoprotection effect and the precise mechanisms of propofol in sepsis-induced renal injury remain unclear. The purpose of the present study was to investigate the role of miR-290-5p/CCL-2 signal...
Lesión Renal Aguda/etiología,
Lesión Renal Aguda/prevención & control,
Western Blotting,
Quimiocina CCL2/efectos de los fármacos,
Quimiocina CCL2/metabolismo,
Citometría de Flujo,
MicroARNs/efectos de los fármacos,
MicroARNs/fisiología,
Propofol/farmacología,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Sepsis/complicaciones,
Sepsis/metabolismo,
Transducción de Señal/efectos de los fármacos
The tumor suppressive role of oridonin, an active compound extracted from Rabdosia rubescens, has been proven in several gastric cancer (GC) cell lines. The present study aimed to evaluate the effect of oridonin on another GC cell line, SNU-216, and explore the potential mechanisms. The viable cell numbe...
Antineoplásicos/farmacología,
Apoptosis/efectos de los fármacos,
Western Blotting,
Carcinoma/tratamiento farmacológico,
Carcinoma/metabolismo,
Carcinoma/patología,
Caspasa 3/análisis,
Caspasa 9/análisis,
Línea Celular Tumoral,
Proliferación Celular/efectos de los fármacos,
Supervivencia Celular/efectos de los fármacos,
Daño del ADN/efectos de los fármacos,
Diterpenos de Tipo Kaurano/farmacología,
Citometría de Flujo,
Células HEK293,
Reproducibilidad de los Resultados,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Neoplasias Gástricas/tratamiento farmacológico,
Neoplasias Gástricas/metabolismo,
Neoplasias Gástricas/patología,
Proteína p53 Supresora de Tumor/análisis
BACKGROUND The genus Flavivirus includes a variety of medically important viruses, including dengue virus (DENV) and Zika virus (ZIKV), which are most prevalent in Brazil. Because the clinical profile of patients affected by different DENV serotypes or ZIKV may be similar, the development of new methods...
Angiogenesis is one of the key processes in the growth and development of tumors. Class-3 semaphorins (Sema3) are characterized as axon guidance factors involved in tumor angiogenesis by interacting with the vascular endothelial growth factor signaling pathway. Sema3 proteins convey their regulatory sign...
Inhibidores de la Angiogénesis/análisis,
Inhibidores de la Angiogénesis/genética,
Línea Celular,
Furina/análisis,
Furina/genética,
Células Endoteliales de la Vena Umbilical Humana,
Neovascularización Patológica/genética,
Plásmidos,
Mutación Puntual/genética,
Valores de Referencia,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Semaforinas/análisis,
Semaforinas/genética,
Factores de Tiempo,
Transfección
MicroRNAs play a crucial role in the progression of spinal cord ischemia/reperfusion injury (SCII). The role of miR-448 and SIRT1 in SCII was investigated in this study, to provide further insights into prevention and improvement of this disorder. In this study, expressions of miR-448 and SIRT1 protein w...
Apoptosis,
Western Blotting,
Modelos Animales de Enfermedad,
Regulación hacia Abajo/fisiología,
Citometría de Flujo,
MicroARNs/metabolismo,
Ratas Sprague-Dawley,
Daño por Reperfusión/metabolismo,
Daño por Reperfusión/fisiopatología,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Sirtuina 1/metabolismo,
Isquemia de la Médula Espinal/metabolismo,
Isquemia de la Médula Espinal/fisiopatología,
Transfección,
Regulación hacia Arriba/fisiología
Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34+ cells from different stages o...
Clinical manifestations of Zika, dengue, and chikungunya virus infections are very similar, making it difficult to reach a diagnosis based only on clinical grounds. In addition, there is an intense cross-reactivity between antibodies directed to Zika virus and other flaviviruses, and an accurate Zika dia...
Farnesoid X receptor (FXR) and related pathways are involved in the therapeutic effect of sleeve gastrectomy for overweight or obese patients with diabetes mellitus. This study aimed to investigate the mechanism of FXR expression regulation during the surgical treatment of obese diabetes mellitus by slee...
Western Blotting,
Diabetes Mellitus Experimental/metabolismo,
Gastrectomía/métodos,
Regulación de la Expresión Génica,
Metabolismo de los Lípidos,
Hígado/metabolismo,
Factor de Transcripción MafB/genética,
Factor de Transcripción MafB/metabolismo,
Obesidad/metabolismo,
Obesidad/cirugía,
Proteínas Oncogénicas/genética,
Proteínas Oncogénicas/metabolismo,
Ratas Sprague-Dawley,
Receptores Citoplasmáticos y Nucleares/genética,
Receptores Citoplasmáticos y Nucleares/metabolismo,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
