Results: 496

Desenvolvimento de método rápido de detecção de gene de resistência a claritromicina para o grupo Mycobacterium abscessus por PCR em tempo real

O grupo Mycobacterium abscessus (MAG) é a principal causa de infecções pulmonares e extrapulmonares entre as micobactérias de crescimento rápido. Para determinar a resistência à claritromicina (CLA), o método de concentração inibitória mínima (CIM) é realizado, no entanto, as leituras são f...

Influência de laminados cerâmicos cimentados sobre dentes naturais sem término cervical na microbiota do fluido gengival crevicular: estudo clínico, prospectivo e longitudinal usando PCR em tempo real

Objetivo: quantificar bactérias colonizadoras do sulco gengival em paralelo à verificação do comportamento clínico periodontal e da adaptação marginal de laminados cerâmicos cimentados sobre dentes sem término cervical. Métodos: 73 laminados cerâmicos em dissilicato de lítio monolítico foram...

Knockdown of long non-coding RNA HOTAIR reverses cisplatin resistance of ovarian cancer cells through inhibiting miR-138-5p-regulated EZH2 and SIRT1

Biol. Res; 53 (), 2020
BACKGROUND: Cisplatin resistance (DDP-resistance) remains one of the major causes of poor prognosis in females with ovarian cancer. Long non-coding RNAs (lncRNAs) have been shown to participate in the regulation of cellular processes, including chemoresistance. The aim of this study was to explore the ro...

Genome-wide characterization of the abscisic acid-, stress- and ripening-induced (ASR) gene family in wheat (Triticum aestivum L)

Biol. Res; 53 (), 2020
BACKGROUND: Abscisic acid-, stress-, and ripening-induced (ASR) genes are a class of plant specific transcription factors (TFs), which play important roles in plant development, growth and abiotic stress responses. The wheat ASRs have not been described in genome-wide yet. METHODS: We predicted the trans...

Overexpression of bHLH domain of HIF-1 failed to inhibit the HIF-1 transcriptional activity in hypoxia

Biol. Res; 53 (), 2020
BACKGROUND: Hypoxia inducible factor-1 (HIF-1) is considered as the most activated transcriptional factor in response to low oxygen level or hypoxia. HIF-1 binds the hypoxia response element (HRE) sequence in the promoter of different genes, mainly through the bHLH domain and activates the transcription ...

Monitoring of Newcastle disease in poultry at migratory birds landing sites: Mangue Seco and Cacha Pregos between 2013 and 2014

Arq. Inst. Biol; 87 (), 2020
The poultry industry has been considered one of the most efficient agricultural activities, placing Brazil in the ranking of the largest producers of chicken meat. However, a threat to the poultry production chain is the entrance of Newcastle disease virus (NDV) in the country, which would bring huge eco...

Epidemiology of Giardia duodenalis assemblages in Brazil: there is still a long way to go

Mem. Inst. Oswaldo Cruz; 115 (), 2020
Giardia duodenalis infection is distributed worldwide and can achieve prevalence around 60%, especially in developing countries. This protozoan is divided into eight assemblages, in which A and B have high zoonotic potential, whereas C to H are host-specific. This scenario is changing as molecular studie...

Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens

Mem. Inst. Oswaldo Cruz; 115 (), 2020
We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). From...

Risk Factors for Early Childhood Caries Based on Identification of Veillonella spp. Using RT-PCR

Abstract Objective: To identify the occurrence of Veillonella spp. in children using real-time PCR (RT-PCR) and to determine its role as a risk factor for ECC in children aged 2-3 years. Material and Methods: A cross-sectional survey was conducted and samples from 87 children aged 2-3 years, who lived ...

Chronic Periodontitis in Patients with Type 2 Diabetes: Analysis of the FokI Polymorphism and Perception of Quality of Life

Abstract Objective: To analyze whether the FokI polymorphism (rs228570) present in the vitamin D receptor gene in type 2 diabetics is related to chronic periodontitis's clinical status and evaluates the influence of chronic periodontitis on the perception of quality of life. Material and Methods: It is...