Abstract The biodiversity and evolution of the microbial community in açai fruits (AF) between three geographical origins and two spontaneous decay conditions were examined by applying culture-independent methods. Culture-independent methods based on 16S rRNA from fifteen samples revealed that Proteobac...
Bacterias/clasificación,
Bacterias/genética,
Bacterias/aislamiento & purificación,
Bacterias/metabolismo,
Biodiversidad,
Euterpe/química,
Euterpe/microbiología,
Frutas/química,
Frutas/microbiología,
Secuenciación de Nucleótidos de Alto Rendimiento,
Microbiota,
Filogenia
Abstract In this study, the development and assessment of a modified, efficient, and cost-efficient protocol for mDNA (metagenomic DNA) extraction from contaminated water samples was attempted. The efficiency of the developed protocol was investigated in comparison to a well-established commercial kit (E...
Métodos Analíticos de la Preparación de la Muestra/economía,
Métodos Analíticos de la Preparación de la Muestra/métodos,
Bacterias/clasificación,
Bacterias/genética,
Bacterias/aislamiento & purificación,
ADN Bacteriano/genética,
ADN Bacteriano/aislamiento & purificación,
Agua Dulce/química,
Agua Dulce/microbiología,
Metagenómica/economía,
Metagenómica/métodos,
Filogenia,
Análisis de Secuencia de ADN
Abstract In the previous study, we used genome shuffling to improve fengycin production of the original strain Bacillus amyloliquefaciens ES-2-4. After two rounds of genome shuffling, a high-yield recombinant FMB72 strain that exhibited 8.30-fold increase in fengycin production was obtained. In this stud...
Bacillus amyloliquefaciens/química,
Bacillus amyloliquefaciens/genética,
Bacillus amyloliquefaciens/metabolismo,
Proteínas Bacterianas/química,
Proteínas Bacterianas/genética,
Proteínas Bacterianas/metabolismo,
Barajamiento de ADN,
Genoma Bacteriano,
Lipopéptidos/biosíntesis,
Proteómica,
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
ABSTRACT Gallesia integrifolia (Phytolaccaceae) is native to Brazil and has a strong alliaceous odor. The objective of this study was to identify the chemical composition of G. integrifolia fruit essential oil and evaluate fungicidal activity against the main food-borne diseases and food spoilage fungi. ...
Aspergillus/efectos de los fármacos,
Aspergillus/crecimiento & desarrollo,
Brasil,
Frutas/química,
Fungicidas Industriales/química,
Fungicidas Industriales/farmacología,
Cromatografía de Gases y Espectrometría de Masas,
Pruebas de Sensibilidad Microbiana,
Aceites Volátiles/química,
Aceites Volátiles/farmacología,
Penicillium/efectos de los fármacos,
Penicillium/crecimiento & desarrollo,
Phytolaccaceae/química,
Aceites de Plantas/química,
Aceites de Plantas/farmacología
Abstract Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed. As found in this study, an ALM with dimensions of 20 × 20 × 5 mm3 is effective for cell immobilization...
Alginatos/química,
Células Inmovilizadas/química,
Células Inmovilizadas/metabolismo,
Etanol/análisis,
Etanol/metabolismo,
Fermentación,
Microbiología Industrial/métodos,
Saccharomyces cerevisiae/química,
Saccharomyces cerevisiae/metabolismo,
Sorghum/química,
Sorghum/metabolismo,
Sorghum/microbiología
ABSTRACT Fusarium oxysporum f. sp. lycopersici is a phytopathogenic fungus that causes vascular wilt in tomato plants. In this work we analyze the influence of metal salts such as iron and copper sulphate, as well as that of bathophenanthrolinedisulfonic acid (iron chelator) and bathocuproinedisulfonic a...
Cobre/metabolismo,
Proteínas Fúngicas/química,
Proteínas Fúngicas/genética,
Proteínas Fúngicas/metabolismo,
Fusarium/química,
Fusarium/enzimología,
Fusarium/genética,
Hierro/metabolismo,
Lacasa/química,
Lacasa/genética,
Lacasa/metabolismo,
Solanum lycopersicum/microbiología,
Enfermedades de las Plantas/microbiología
Cell cycle control genes are frequently mutated in cancer cells, which usually display higher rates of proliferation than normal cells. Dysregulated mitosis leads to genomic instability, which contributes to tumor progression and aggressiveness. Many drugs that disrupt mitosis have been studied because t...
OBJECTIVES: Advancements in non-small cell lung cancer treatment based on targeted therapies have made the differentiation between adenocarcinoma and squamous cell carcinoma increasingly important. Pathologists are challenged to make the correct diagnosis in small specimens. We studied the accuracy of an...
Adenocarcinoma/química,
Adenocarcinoma/patología,
Biomarcadores de Tumor/análisis,
Biopsia,
Carcinoma de Pulmón de Células no Pequeñas/patología,
Carcinoma de Células Escamosas/química,
Carcinoma de Células Escamosas/patología,
Diagnóstico Diferencial,
Inmunohistoquímica,
Neoplasias Pulmonares/química,
Neoplasias Pulmonares/patología,
Estudios Retrospectivos
Osteosarcoma (OS) has a high incidence, malignity, and frequency of recurrence and metastasis. In this study, we aimed to explore the potential anti-cancer effects of Astragalus polysaccharides (APS) on human OS MG63 cells as well as underlying mechanisms. Viability of MG63 cells was assessed by CCK-8 as...
Análisis de Varianza,
Antineoplásicos/farmacología,
Apoptosis/efectos de los fármacos,
Planta del Astrágalo/química,
Western Blotting,
Neoplasias Óseas/tratamiento farmacológico,
Neoplasias Óseas/patología,
Ciclo Celular/efectos de los fármacos,
Línea Celular Tumoral,
Movimiento Celular/efectos de los fármacos,
Proliferación Celular/efectos de los fármacos,
Supervivencia Celular/efectos de los fármacos,
Proteínas Quinasas JNK Activadas por Mitógenos/análisis,
MicroARNs/análisis,
Reproducibilidad de los Resultados,
Regulación hacia Arriba/efectos de los fármacos
This study was designed to provide laboratory evidence supporting the hematopoietic effect of Beta vulgaris (beet) leaf aqueous extract in phenylhydrazine-induced anemia model in albino rats. Extraction of the leaves/stalks was done by maceration in 30% hydro-ethanol for 48 h. An intraperitoneal injectio...
