Results: 222

Isolation of breast cancer stem cell from MDA-MB231 cell line using vincristine

Int. j. morphol; 34 (4), 2016
Cancer has been considered as a stem cell disease. Suspension culture combined with anti-cancer drugs has recently been proposed for isolation of cancer stem cells (CSCs). In the current study, Vincristine as an anti-cancer drug combined with suspension culture was used for isolation and purification of ...

Mayaro fever in an HIV-infected patient suspected of having Chikungunya fever

Abstract Arboviruses impose a serious threat to public health services. We report a case of a patient returning from a work trip to the Amazon basin with myalgia, arthralgia, fever, and headache. During this travel, the patient visited riverside communities. Both dengue and Chikungunya fevers were first ...

Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR

Arq. neuropsiquiatr; 74 (10), 2016
ABSTRACT The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain rea...

Isolation of high-quality RNA from Platycladus orientalis and other Cupressaceae plants

Electron. j. biotechnol; 19 (5), 2016
Platycladus orientalis has a lifespan of several thousand years in China, making it a good plant in which to study aging at the molecular level, but this requires sufficient quantities of high-quality P. orientalis RNA. However, no appropriate methods have been reported for total RNA isolation from P. or...

Association of genetic polymorphisms of CISH with the risk of pulmonary tuberculosis in Zahedan, Southeast Iran

Braz. j. infect. dis; 20 (4), 2016
Abstract Background In the current study we aimed to find out the impact of cytokine-inducible Src homology 2 domain protein (CISH) gene polymorphisms on the risk of pulmonary tuberculosis (PTB) in a sample of Iranian population. Materials and methods Polymorphisms of CISH rs2239751, rs414171, and rs67...

Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil

Abstract: INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance. Here, we evaluate and optimize a quantitative real-time rev...

Dengue as a cause of fever during pregnancy: a report of two cases

Abstract: Dengue infection has not been routinely investigated among pregnant women and parturients with acute febrile syndrome in endemic settings. Here, we report two cases of dengue fever detected at the time of delivery in parturients enrolled in a cohort prospective study conducted in a hospital in ...

Expresión génica del factor de crecimiento transformante beta en niños con fisura labio palatina no sindrómica

El objetivo fue determinar la expresión génica del factor de crecimiento transformante beta (TGF-ß) y posibles factores de riesgo en niños con y sin fisuras labio palatina no sindrómicas (FLPNS). Diseño de casos (Niños con fisuras orales; n= 20) y controles (niños no afectados; n= 40). A partir d...

Detección de bocavirus humano en la población infantil de Tucumán y Santa Fe, Argentina

Rev. chil. infectol; 33 (2), 2016
Background: A large proportion of acute respiratory tract infections (ARTI) remain without etiologic diagnosis, reason why new pathogens are investigated continuously. Human bocavirus (HBoV) was discovered in 2005, as a new member of Parvoviridae family and proposed to cause ARTI. Aim: To know the preval...

Combined effects of melatonin and FGF-2 on mouse preosteoblast behavior within interconnected porous hydroxyapatite ceramics - in vitro analysis

J. appl. oral sci; 24 (2), 2016
ABSTRACT Objective Biocompatible materials such as interconnected porous hydroxyapatite ceramics (IP-CHA) loaded with osteogenic cells and bioactive agents are part of an evolving concept for overcoming craniofacial defects by use of artificial bone tissue regeneration. Amongst the bioactive agents, me...