Background: The salivary glands of Lucilia sericata are the first organs to express specific endopeptidase enzymes. These enzymes play a central role in wound healing, and they have potential to be used therapeutically. Methods: Rapid amplification of cDNA ends and rapid amplification of genomic ends wer...
Background: Ornithine decarboxylase antizyme 1 (OAZ1) is an important regulator of polyamine synthesis and uptake. Our previous studies indicated that high OAZ1 expression in the ovaries of laying geese is responsible for poor egg production. In the present study, the molecular characterization of goose ...
Proteínas/metabolismo,
Proteínas/genética,
Gansos/metabolismo,
Análisis de Secuencia,
Clonación Molecular,
Folículo Ovárico/metabolismo,
Folículo Ovárico/crecimiento & desarrollo,
Poliaminas/metabolismo,
ADN Complementario,
ARN Mensajero,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Ornitina Descarboxilasa/metabolismo
BACKGROUND: Cocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulati...
Abstract The open reading frame of a Brazilian bovine viral diarrhea virus (BVDV) strain, IBSP4ncp, was recombined with the untranslated regions of the reference NADL strain by homologous recombination in Saccharomyces cerevisiae, resulting in chimeric full-length cDNA clones of BVDV (chi-NADL/IBSP4ncp#2...
ADN Complementario,
Virus de la Diarrea Viral Bovina/genética,
Genoma Viral,
Recombinación Homóloga,
Levaduras/genética,
Línea Celular,
Virus de la Diarrea Viral Bovina/fisiología,
Virus de la Diarrea Viral Bovina/ultraestructura,
Sistemas de Lectura Abierta,
Análisis de Secuencia de ADN,
Replicación Viral,
Levaduras/metabolismo
Abstract Background: Thyroid hormone deficiency during fetal life could affect the cardiac function in later life. The mechanism underlying this action in fetal hypothyroidism (FH) in rats has not been elucidated thus far. Objective: The aim of this study is to evaluation the effect of FH on cardiac ...
Antitiroideos,
Peso Corporal/efectos de los fármacos,
Hipotiroidismo Congénito/sangre,
Hipotiroidismo Congénito/inducido químicamente,
Hipotiroidismo Congénito/metabolismo,
ADN Complementario/metabolismo,
Modelos Animales de Enfermedad,
Frecuencia Cardíaca,
Miocardio/metabolismo,
Cadenas Pesadas de Miosina/metabolismo,
Propiltiouracilo,
ARN Mensajero/metabolismo,
Distribución Aleatoria,
Ratas Wistar,
Tiroxina/sangre,
Triyodotironina/sangre,
Presión Ventricular
Introdução: As neoplasias das glândulas salivares têm amplo espectro histológico resultante da múltipla diferenciação celular tumoral. O adenoma pleomórfico (AP) e o carcinoma adenoide cístico (CAC) são as mais comuns neoplasias benignas e malignas provenientes do ducto intercalado, respectiva...
Neoplasias de las Glándulas Salivales/genética,
Adenoma Pleomórfico/genética,
Proteínas Proto-Oncogénicas c-kit/genética,
Mutación,
Carcinoma Adenoide Quístico/genética,
Expresión Génica,
Regulación de la Expresión Génica,
Neoplasias de las Glándulas Salivales/metabolismo,
Neoplasias de las Glándulas Salivales/patología,
Adenoma Pleomórfico/metabolismo,
Adenoma Pleomórfico/patología,
Carcinoma Adenoide Quístico/metabolismo,
Carcinoma Adenoide Quístico/patología,
Proteínas Proto-Oncogénicas c-kit/metabolismo,
Proteínas Proto-Oncogénicas c-kit/fisiología,
Proteínas Proto-Oncogénicas/metabolismo,
Proteínas Proto-Oncogénicas/fisiología,
MicroARNs,
ADN Complementario,
Análisis de Supervivencia
Background Hepcidins, a kind of cysteine-rich antimicrobial peptides, play important roles in host immunological processes and iron regulation, which have been identified from several fish species. The rare minnow (Gobiocypris rarus), an endemic cyprinid fish in China, has been used extensively as model ...
Background Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and inves...
Ácido Graso Sintasas/genética,
Ácido Graso Sintasas/metabolismo,
Lipogénesis,
Tejido Adiposo/metabolismo,
Diferenciación Celular,
Ácido Oléico,
Patos,
Análisis de Secuencia,
ARN/análisis,
ADN Complementario/síntesis química,
Supervivencia Celular,
Expresión Génica,
Biología Computacional,
Filogenia,
Clonación Molecular
Background Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to analysis...
Musa/genética,
Clonación Molecular,
Proteínas de Plantas/genética,
Ácido Abscísico,
Reguladores del Crecimiento de las Plantas,
ADN Complementario/síntesis química,
Estrés Salino,
Proteínas de Plantas/metabolismo,
Arabidopsis,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Análisis de Secuencia,
ARN/análisis,
Plantas Modificadas Genéticamente,
Tolerancia a la Sal
Background Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic and molluscicidal. But not much is known about the pat...
Jatropha/enzimología,
Isomerasas de Doble Vínculo Carbono-Carbono/metabolismo,
Isomerasas de Doble Vínculo Carbono-Carbono/genética,
Hemiterpenos/metabolismo,
Hemiterpenos/genética,
Southern Blotting,
Clonación Molecular,
Jatropha/química,
ADN Complementario/síntesis química,
ARN/aislamiento & purificación,
Filogenia,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Expresión Génica,
Cloroplastos