Results: 25

Detección de Acanthamoeba por reacción en cadena de la polimerasa en tiempo real en líquidos de conservación de lentes de contacto
Detection of Acanthamoeba sp. by real-time polymerase chain reaction in contact lens solutions

Rev. cuba. oftalmol; 33 (1), 2020
RESUMEN Objetivo: Estandarizar una técnica de reacción en cadena de la polimerasa en tiempo real para la detección del parásito e identificar Acanthamoeba en líquidos conservantes de lentes de contacto. Métodos: Se realizó un estudio descriptivo observacional de corte transversal sobre la técn...

Infectious keratitis in southern Brazil: a comparison culture negative and culture positive patients

Rev. bras. oftalmol; 79 (1), 2020
Abstract Purpose: To compare clinical-epidemiological profile and treatment outcome between culture negative and culture positive keratitis patients. Methods: Patients with suspected infectious keratitis seen at two ophthalmic hospitals in Curitiba, Brazil, between June 2014 and April 2016, were prospe...

Estudo epidemiológico das queratites infeciosas internadas num centro hospitalar terciário - revisão de 5 anos

Rev. bras. oftalmol; 78 (6), 2019
Resumo Objetivo: A queratite infeciosa é uma doença de incidência relativamente elevada e é responsável por um número importante de internamentos. Neste estudo pretende-se estudar diversas características epidemiológicas e clínicas associadas às queratites infeciosas de alto risco num hospital...

The first Acanthamoeba keratitis case in the Midwest region of Brazil: diagnosis, genotyping of the parasite and disease outcome

Abstract We report an Acanthamoeba keratitis case associated with the use of contact lens in a 28-year-old female from Brasília, Brazil. Samples from corneal scraping and contact lens case were used for culture establishment, PCR amplification, and partial sequencing (fragments of ~400kb) of small subun...

Rio Negro virophage: Sequencing of the near complete genome and transmission electron microscopy of viral factories and particles

Braz. j. microbiol; 49 (supl.1), 2018
ABSTRACT Rio Negro virophage (RNV) was co-isolated with a strain of mimivirus named sambavirus, from Brazilian Amazon. We report the near complete genome sequence of RNV, the first virophage isolated in Brazil. We also present new microscopical data demonstrating that RNV particles have similar dimension...

Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers

Mem. Inst. Oswaldo Cruz; 112 (12), 2017
BACKGROUND Acanthamoeba is the genus of free-living amoebae that is most frequently isolated in nature. To date, 20 Acanthamoeba genotypes have been described. Genotype T4 is responsible for approximately 90% of encephalitis and keratitis cases. Due to the ubiquitous presence of amoebae, isolation from e...

Presencia de Acanthamoeba spp. en agua para consumo ganadero en la provincia de La Pampa, Argentina

Rev. argent. microbiol; 49 (3), 2017
Las amebas de vida libre del género Acanthamoeba incluyen cepas patógenas y no patógenas que actualmente se encuentran clasificadas en 18 genotipos diferentes (T1-T18). Se llevó a cabo un relevamiento para determinar la presencia de cepas de Acanthamoeba en agua de bebida para consumo ganadero en la ...

Epidemiological profile of infectious keratitis

Rev. bras. oftalmol; 76 (3), 2017
Abstract Objective: To evaluate the epidemiologic aspects of cornea ulcers in a referred ophthalmology private practice center. Methods: Retrospective study over electronic files of patients treated for corneal ulcers during a period of 7 years by the same physician (RJMF) at Centro de Olhos São Franc...

Microbiological and epidemiological study of infectious keratitis in children and adolescents

Arq. bras. oftalmol; 79 (5), 2016
ABSTRACT Purpose: To analyze epidemiological and microbiological aspects of microbial keratitis in children and adolescents. Methods: This retrospective cohort study was conducted at the Department of Ophthalmology and Visual Science, Escola Paulista de Medicina, Universidade Federal de São Paulo, bet...

Hallazgo de acanthamoeba spp en biopsia cerebral de un paciente con adenocarcinoma metastásico
Acanthamoeba spp found in a cerebral biopsy from a patient with metastasic adenocarcinoma

Rev. Inst. Nac. Hig; 42 (2), 2011
Las amibas de vida libre (AVL) del género Acanthamoeba son parásitos facultativos ampliamente distribuidos en el ambiente, por lo cual el contacto del humano con ellos es frecuente, sin embargo, los reportes de enfermedades producidas por AVL son escasos. La gravedad de éstas justifica su búsqueda, s...