BACKGROUND Trypanosoma cruzi is a protozoan parasite and an etiological agent of Chagas disease. There is a wide variability in the clinical outcome of its infection, ranging from asymptomatic individuals to those with chronic fatal mega syndromes. Both parasite and host factors, as well as their interpl...
Abstract INTRODUCTION: The production of the Montenegro antigen for skin test poses difficulties regarding quality control. Here, we propose that certain animal models reproducing a similar immune response to humans may be used in the quality control of Montenegro antigen production. METHODS: Fifteen C...
BACKGROUND The surface of infected red blood cells (iRBCs) has been widely investigated because of the molecular complexity and pathogenesis mechanisms involved. Asymptomatic individuals are important in the field because they can perpetuate transmission as natural reservoirs and present a challenge for ...
Anticuerpos Antiprotozoarios/genética,
Anticuerpos Antiprotozoarios/inmunología,
Antígenos de Protozoos/genética,
Antígenos de Protozoos/inmunología,
Infecciones Asintomáticas,
Western Blotting,
Electroforesis en Gel Bidimensional,
Membrana Eritrocítica/inmunología,
Membrana Eritrocítica/parasitología,
Espectrometría de Masas,
Plasmodium falciparum/genética,
Plasmodium falciparum/inmunología,
Proteómica
Duarte, Mariana Costa;
Lage, Daniela Pagliara;
Martins, Vívian Tamietti;
Chávez-Fumagalli, Miguel Angel;
Roatt, Bruno Mendes;
Menezes-Souza, Daniel;
Goulart, Luiz Ricardo;
Soto, Manuel;
Tavares, Carlos Alberto Pereira;
Coelho, Eduardo Antonio Ferraz.
Abstract: Visceral leishmaniasis (VL) is one of the most important tropical diseases worldwide. Although chemotherapy has been widely used to treat this disease, problems related to the development of parasite resistance and side effects associated with the compounds used have been noted. Hence, alternat...
It was designed and characterized a reporter system to be captured by antibodies bound to ELISA plates. The system was designed with the rK346 from Leishmania infantum, a highly antigenic and specific protein. The rK346 was coupled to the horseradish peroxidase C (HRPc) from Armoracia rusticana using glu...
ABSTRACT Visceral Leishmaniasis, also know as Kala-azar, is a parasitic tropical disease caused by protozoa of the genus Leishmania donovani. It is an endemic disease in many countries. It affects approximately 1,5 million people every year, and when associated with mal-nutrition and co-infection it may ...
Leishmaniasis Visceral/complicaciones,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/tratamiento farmacológico,
Infecciones Parasitarias del Ojo/etiología,
Infecciones Parasitarias del Ojo/diagnóstico,
Infecciones Parasitarias del Ojo/tratamiento farmacológico,
Hemorragia Retiniana/etiología,
Hemorragia Retiniana/diagnóstico,
Hemorragia Retiniana/tratamiento farmacológico,
Pruebas Serológicas/métodos,
Antígenos de Protozoos/inmunología,
Anticuerpos Antiprotozoarios/sangre,
Proteínas Protozoarias,
Oftalmoscopía,
Anfotericina B/uso terapéutico,
Angiografía con Fluoresceína,
Hepatomegalia,
Esplenomegalia,
Pancitopenia
This study aimed to express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain; test this protein as an antigen in serological assays; and investigate its antigenicity and immunogenicity. A protein coded by an allele of the A2 gene isolated from L. chagasi was expressed in three di...
Antígenos de Protozoos/sangre,
Enfermedades de los Perros/sangre,
Enfermedades de los Perros/diagnóstico,
Leishmania infantum/inmunología,
Leishmania infantum/metabolismo,
Leishmaniasis Visceral/sangre,
Leishmaniasis Visceral/veterinaria,
Proteínas Protozoarias/biosíntesis,
Proteínas Protozoarias/sangre,
Proteínas Recombinantes/biosíntesis,
Proteínas Recombinantes/análisis,
Pruebas Serológicas
Neosporosis is a disease caused by the protozoon Neospora caninum that leads to significant economic losses in many countries. In the present study, we report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing ne...
Antígenos de Protozoos/biosíntesis,
Antígenos de Protozoos/sangre,
Antígenos de Protozoos/inmunología,
Antígenos de Superficie/biosíntesis,
Antígenos de Superficie/sangre,
Antígenos de Superficie/inmunología,
Enfermedades de los Perros/sangre,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/parasitología,
Ensayo de Inmunoadsorción Enzimática,
Neospora/inmunología,
Pichia/metabolismo,
Infecciones Protozoarias en Animales/sangre,
Infecciones Protozoarias en Animales/diagnóstico,
Proteínas Protozoarias/biosíntesis,
Proteínas Protozoarias/sangre,
Proteínas Protozoarias/inmunología,
Ovinos,
Enfermedades de las Ovejas/sangre,
Enfermedades de las Ovejas/diagnóstico,
Enfermedades de las Ovejas/parasitología
Apt B, Werner;
Heitmann G, Ingrid;
Jercic L, M. Isabel;
Jofré M, Leonor;
Muñoz C. del V, Patricia;
Noemí H, Isabel;
San Martín V, Ana M;
Sapunar P, Jorge;
Torres H, Marisa;
Zulantay A, Inés.
In this fifth part of Guidelines for Chagas disease, diagnostic techniques for Trypanosoma cruzi infection in humans are reviewed, the interpretation of laboratory results and an algorithm for laboratory diagnosis in immunocompetent hosts are presented. Chagas disease may be diagnosed by three kinds of t...
