BACKGROUND/AIMS: Hypoxia microenvironment plays a crucial role during tumor progression and it tends to exhibit poor prognosis and make resistant to various conventional therapies. HIF-1α acts as an important transcriptional regulator directly or indirectly associated with genes involved in cell prolife...
Apoptosis/efectos de los fármacos,
Hipoxia de la Célula/efectos de los fármacos,
Cobalto/farmacología,
Apoptosis/genética,
Hipoxia de la Célula/genética,
Proliferación Celular/efectos de los fármacos,
Western Blotting,
Línea Celular Tumoral,
Citometría de Flujo,
Regulación Neoplásica de la Expresión Génica,
Células MCF-7,
Transfección
Abstract Purpose: To analyze the effects of ischemic preconditioning (IPC) in the expression of apoptosis-related genes in rat small intestine subjected to ischemia and reperfusion. Methods: Thirty anesthetized rats underwent laparotomy and were drive into five groups: control (CG); ischemia (IG); is...
Apoptosis/genética,
Proteínas Reguladoras de la Apoptosis/análisis,
Proteínas Reguladoras de la Apoptosis/genética,
Constricción,
Regulación hacia Abajo,
Células Endoteliales/patología,
Expresión Génica,
Precondicionamiento Isquémico/métodos,
Yeyuno/irrigación sanguínea,
Yeyuno/patología,
Arteria Mesentérica Superior,
Isquemia Mesentérica/genética,
Isquemia Mesentérica/patología,
Distribución Aleatoria,
Ratas Wistar,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Valores de Referencia,
Daño por Reperfusión/prevención & control,
Reproducibilidad de los Resultados
ABSTRACT Considering aging as a phenomenon in which there is a decline in essential processes for cell survival, we investigated the autophagic and proteasome pathways in three different groups: young, older and oldest old male adults. The expression profile of autophagic pathway-related genes was carrie...
Envejecimiento/genética,
Envejecimiento/metabolismo,
Apoptosis/genética,
Autofagia/genética,
Autofagia/fisiología,
Brasil,
Regulación de la Expresión Génica,
Longevidad/genética,
Longevidad/fisiología,
Complejo de la Endopetidasa Proteasomal/genética,
Complejo de la Endopetidasa Proteasomal/metabolismo
Abstract Objective The aim of the present study was to analyze the expression of the CD63, S100A6, and GNB2L1genes, which participate in mechanisms related to the complex pathophysiology of endometriosis. Methods A case-control study was conducted with 40 women who were diagnosed with endometriosis, ...
Apoptosis/genética,
Estudios de Casos y Controles,
Proteínas de Ciclo Celular/genética,
Proliferación Celular/genética,
Endometriosis/genética,
Endometriosis/patología,
Expresión Génica,
Proteínas de Neoplasias/genética,
Neovascularización Patológica/genética,
Receptores de Cinasa C Activada/genética,
Proteína A6 de Unión a Calcio de la Familia S100/genética,
Tetraspanina 30/genética
Calastri, Maria Clara Jessica;
Rodrigues, Nicolas Luz Toledo Ortega;
Hatori, Gabriela;
Gregório, Michele Lima;
Brancati, Camila Ive Ferreira Oliveira;
Zanovelo, Eliane Milharcix;
Ferraz Filho, José Roberto Lopes;
Neiva, Cassiano Merussi;
Rodrigues Junior, Antonio Carlos Ponde;
Godoy, Moacir Fernandes de;
Lancelloti, Carmen Lucia Penteado;
Tognola, Waldir Antonio;
Souza, Dorotéia Rossi Silva.
ABSTRACT Background Glioma, the most common primary malignant brain tumor in adults, is highly aggressive and associated with a poor prognosis. The objectives of this study were to evaluate the association of genetic polymorphisms related to angiogenesis and apoptosis with gliomas, as well as comorbidi...
Antineoplásicos Alquilantes/administración & dosificación,
Apoptosis/genética,
Neoplasias Encefálicas/genética,
Neoplasias Encefálicas/patología,
Neoplasias Encefálicas/terapia,
Terapia Combinada,
Dacarbazina/administración & dosificación,
Dacarbazina/análogos & derivados,
Genotipo,
Glioma/genética,
Glioma/patología,
Glioma/terapia,
Estimación de Kaplan-Meier,
Estilo de Vida,
Neovascularización Patológica,
Polimorfismo Genético/genética,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Temozolomida
SUMMARY: Toxic effects of anti-cancer and other drugs on the normal tissues could be reduced by the herbal plants and their fractions. This study investigated the protective effect of Tribulus terrestris (TT) on Cisplatin- induced cytotoxicity germ cell apoptosis in male mice. In this experimental study,...
Among the novel class of endogenous long non-coding RNAs, circular RNA (circRNA) is known as a key regulator in the development and progression of different cancers. Its function and mechanism in the tumorigenesis of colorectal cancer, however, has not been well studied. This study thus aimed to investig...
Long non-coding RNA antisense non-coding RNA in the INK4 locus (ANRIL) has been reported to promote tumorigenesis via regulating microRNA (miR)-99a in gastric cancer cells. However, the role of each component involved in it is still not well understood. This study aimed to verify the role of ANRIL in gas...
Apoptosis/genética,
Carcinogénesis/genética,
Línea Celular Tumoral,
Regulación hacia Abajo,
Regulación Neoplásica de la Expresión Génica,
MicroARNs/metabolismo,
Invasividad Neoplásica,
ARN Largo no Codificante/genética,
Neoplasias Gástricas/metabolismo,
Neoplasias Gástricas/patología,
Serina-Treonina Quinasas TOR/metabolismo,
Transfección,
Regulación hacia Arriba
Lu, Yuting;
Zhang, Ruili;
Wang, Zhenying;
Zhou, Shuhua;
Song, Yali;
Chen, Lamei;
Chen, Nan;
Liu, Wenmin;
Ji, Canan;
Wu, Wangli;
Zhang, Li.
We constructed lentiviral vectors containing the human wild-type GJB6 gene and the mutant variants A88V and G11R. The three proteins were stably expressed by the Tet-on system in the HaCaT cell line and used to study the functional effect of the variants. The CCK-8 assay and flow cytometric analyses were...
Apoptosis/genética,
Caspasas/metabolismo,
Línea Celular,
Proliferación Celular/efectos de los fármacos,
Proliferación Celular/genética,
Células Cultivadas,
Conexina 30/fisiología,
Doxiciclina/farmacología,
Displasia Ectodérmica/genética,
Citometría de Flujo,
Mutación/efectos de los fármacos
Cardiomyocyte apoptosis plays key roles in the pathogenesis of heart diseases such as myocardial infarction. MicroRNAs are important regulators of gene expression, which are also involved in the regulation of cardiomyocyte apoptosis. However, cardiomyocyte apoptosis regulated by microRNA (miR)-122 is lar...
