The present study had the objective of evaluating the longevity of A. mellifera workers fed on a diet incorporating commercial entomopathogens, Beauveria bassiana, and Bacillus thuringiensis. It also aimed at verifying possible morphological alterations in the midgut. To this purpose, the entomopathogens...
Ponce Guevara, Jovita Francia;
Erazo, Brian M;
Yanes, Néstor J;
Maradiaga, Allison Y;
Bustillo Ponce, Ammi;
Cruz, Ana;
Varela, Diego;
Durón, Dyan C;
Antúnez Williams, Kary Antúnez;
Pedro Maradiaga, Pedro Maradiaga;
Matute, Silvia;
Sandoval, Stephany.
Introducción: El dengue es la enfermedad viral transmitida por mosquitos con la propagación más rápida en el mundo. En Honduras constituye un importante problema de salud pública debido a su alta incidencia. Bacillus thuringiensis var israelensis (Bti) es un agente de control biológico aplicado en ...
Bacillus thuringiensis subsp. israelensis has been used to control the Aedes aegypti (Diptera: Culicidae) mosquito larvae, the vector of virus diseases such as dengue, Chikungunya and Zika fever, which have become a major public health problem in Brazil and other tropical countries since the climate favo...
Preliminary bioassays with whole cultures (WC) of 124 Bacillus thuringiensis strains were performed with neonate larvae of Anthonomus grandis, a major cotton pest in Argentina and other regions of the Americas. Three exotic and four native strains were selected for causing more than 50% mortality. All of...
A total of 268 Bacillus thuringiensis strains obtained from different sources of Argentina were analyzed to determine the diversity and distribution of the cryl, cry2, cry8, cry9 and vip3A genes encoding for lepidopteran-specific insecticidal proteins. Twin strains were excluded. Ten different profiles w...
Bacillus thuringiensis,
Toxinas Bacterianas,
Genes Bacterianos,
Proteínas Hemolisinas,
Argentina,
Bacillus thuringiensis/genética,
Bacillus thuringiensis/patogenicidad,
Proteínas Bacterianas,
Toxinas Bacterianas/genética,
Endotoxinas,
Proteínas Hemolisinas/genética,
Proteínas Hemolisinas/fisiología,
Larva,
Lepidópteros,
Control Biológico de Vectores
Abstract: Background: Chemical pesticides, widely used in agriculture and vector-borne disease control, have shown toxic effects on the environment and the people in contact with them. Bacillus thuringiensis is a widely used bacterium for alternative and safer control of insect pests. Its toxins are sp...
Bacillus thuringiensis,
Proteínas Bacterianas/farmacología,
Endotoxinas/farmacología,
Proteínas Hemolisinas/farmacología,
Insecticidas/farmacología,
Proteómica/Métodos,
Toxinas de Bacillus thuringiensis,
Proteínas Bacterianas/aislamiento & purificación,
ADN Ribosómico/genética,
Endotoxinas/aislamiento & purificación,
Proteínas Hemolisinas/aislamiento & purificación,
Insecticidas/aislamiento & purificación,
Larva/efectos de los fármacos,
México,
Mariposas Nocturnas/efectos de los fármacos,
Control Biológico de Vectores/Métodos
ABSTRACT In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larva...
Bacillus thuringiensis/fisiología,
Proteínas Bacterianas/genética,
Endotoxinas/genética,
Expresión Génica,
Proteínas Hemolisinas/genética,
Control de Insectos,
Bacillus thuringiensis/ultraestructura,
Proteínas Bacterianas/metabolismo,
Proteínas Bacterianas/toxicidad,
Clonación Molecular,
Endotoxinas/metabolismo,
Endotoxinas/toxicidad,
Proteínas Hemolisinas/metabolismo,
Proteínas Hemolisinas/toxicidad,
Control de Insectos/métodos,
Insecticidas,
Larva,
Mariposas Nocturnas/efectos de los fármacos
Background: Bacillus thuringiensis Cry toxins bind with different insect midgut proteins leading to toxin oligomerization, membrane insertion and pore formation. However, different Cry toxins had been shown to readily form high molecular weight oligomers or aggregates in solution in the absence of recept...