The aim of this study was to inhibit adipogenic differentiation by transfecting two growth factors, platelet-derived growth factor (PDGF-BB) and bone morphogenic protein 2 (BMP-2), into modified rat bone marrow mesenchymal stem cells (rBMSCs) and then compounded with platelet-rich plasma (PRP). To achiev...
Abstract Potent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation. Objective This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs)...
Abstract Hyperglycemia, a major characteristic of diabetes, is considered to play a vital role in diabetic complications. High glucose levels have been found to inhibit the mineralization of dental pulp cells. However, gene expression associated with this phenomenon has not yet been reported. This is imp...
Osteoarthritis (OA) is a chronic health condition. MicroRNAs (miRs) are critical in chondrocyte apoptosis in OA. We aimed to investigate the mechanism of miR-130b in OA progression. Bone marrow mesenchymal stem cells (BMSCs) and chondrocytes were first extracted. Chondrogenic differentiation of BMSCs was...
The study evaluated the effect of the supernatant of placental explants from preeclamptic (PE) and normotensive (NT) pregnant women after tissue treatment with or without vitamin D (VD) on oxidative stress and nitric oxide (NO) bioavailability in human umbilical vein endothelial cells (HUVEC). Placental ...
Troncoso, Mayarling Francisca;
Pavez, Mario;
Wilson, Carlos;
Lagos, Daniel;
Duran, Javier;
Ramos, Sebastián;
Barrientos, Genaro;
Silva, Patricio;
Llanos, Paola;
Basualto-Alarcón, Carla;
Westenbrink, B. Daan;
Lavandero, Sergio;
Estrada, Manuel.
BACKGROUND: Testosterone regulates nutrient and energy balance to maintain protein synthesis and metabolism in cardiomyocytes, but supraphysiological concentrations induce cardiac hypertrophy. Previously, we determined that testosterone increased glucose uptake—via AMP-activated protein kinase (AMPK)�...
BACKGROUND: PGF2α is essential for the induction of the corpus luteum regression which in turn reduces progesterone production. Early growth response (EGR) proteins are Cys2-His2-type zinc-finger transcription factor that are strongly linked to cellular proliferation, survival and apoptosis. Rapid eleva...
Búfalos,
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas,
Cuerpo Lúteo/fisiología,
Dinoprost/farmacología,
Proteína 1 de la Respuesta de Crecimiento Precoz/fisiología,
Luteólisis,
Células Cultivadas,
Cuerpo Lúteo/citología,
Regulación de la Expresión Génica,
Transducción de Señal,
Factor de Crecimiento Transformador beta1/fisiología
BACKGROUNDS: Parkinson's disease (PD) is a common age-related neurodegenerative disorder worldwide. This research aimed to investigate the effects and mechanism underlying long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in PD. METHODS: SK-N-SH and SK-N-BE cells were tr...
BACKGROUND: Peroxisome proliferator-activated receptor alpha (PPARα) is associated with diabetic retinopathy (DR), and the underlying mechanism is still unclear. Aim of this work was to investigate the mechanism of PPARα in DR. METHODS: Human retinal capillary pericytes (HRCPs) were treated with high g...
ADN (Citosina-5-)-Metiltransferasa 1/metabolismo,
Retinopatía Diabética,
PPAR alfa/genética,
Retina/patología,
Apoptosis,
Células Cultivadas,
Metilación de ADN,
Diabetes Mellitus,
Modelos Animales de Enfermedad,
Metilación,
Regiones Promotoras Genéticas,
Retina/citología
Resumen Introducción: El jabón para el aseo cutáneo es de empleo común entre la población, sin embargo, es posible que cause daño a las células de la piel y modifique la barrera cutánea. Objetivo: Determinar el efecto citotóxico de los jabones en queratinocitos cultivados in vitro y correlacio...
Agua Corporal,
Células Cultivadas,
Colorimetría,
Dermatitis Irritante/etiología,
Eritema/inducido químicamente,
Voluntarios Sanos,
Concentración de Iones de Hidrógeno,
Irritantes/efectos adversos,
Queratinocitos/efectos de los fármacos,
Piel/efectos de los fármacos,
Pruebas de Irritación de la Piel,
Jabones/efectos adversos,
Jabones/química
