Results: 19

Use of a commercial agarose gel for analysis of urinary glycosaminoglycans in mucopolysaccharidoses

Braz. j. pharm. sci; 52 (4), 2016
ABSTRACT Mucopolysaccharidoses (MPS) are a group of inherited metabolic disorders caused by deficiency of enzymes that degrade glycosaminoglycans (GAGs). Urinary excretion of GAGs is a common feature of MPS, and is considered their major biomarker. We aimed to adapt the GAG electrophoresis method to a co...

Detección de polyomavirus BK y JC en extractos leucocitarios de sangre periférica de pacientes con infección por VIH del área norte de Santiago

Rev. chil. infectol; 33 (3), 2016
Introducción: Polyomavirus BK (BKPyV) y JC (JCPyV) son patógenos persistentes con capacidad de reactivación en inmunocomprometidos, afectando principalmente el sistema urinario y el sistema nervioso central, respectivamente. No existen estudios chilenos en población infectada por VIH. Objetivo: Detec...

Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv. ) Diels varieties of China

Electron. j. biotechnol; 18 (2), 2015
Background Angelica sinensis is a well-known traditional Chinese medicinal plant. We aimed to assess the genetic diversity and relationships in A. sinensis cultivars collected from different locations of China and also some other Angelica species. Results We employed an improved random amplified polymorp...

Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle

Electron. j. biotechnol; 17 (4), 2014
Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recen...

Loop-mediated isothermal amplification assays for screening of bacterial integrons

Biol. Res; 47 (), 2014
BACKGROUND: The occurrence and prevalence of integrons in clinical microorganisms and their role played in antimicrobial resistance have been well studied recently. As screening and detection of integrons are concerned, current diagnostic methodologies are restricted by significant drawbacks and novel me...

Control genético de los sistemas isoenzimáticos isocitrato deshidrogenasa y siquimato deshidrogenasa en el ajonjoli (Sesamum indicum L. )

Acta cient. venez; 53 (3), 2002
Dado que el uso ideal de los marcadores isoenzimáticos requiere del conocimiento de su control genético, este trabajó buscó establecer el modo de herencia y el grado de ligamiento de los loci que codifican la expresión de dos sistemas enzimáticos en el ajonjolí: isocitrato deshidrogenasa (IDH) y s...

Crescimento homogêneo de trichomonas vaginalis em meio semi-sólido
Homogeneous growth of trichomonas vaginalis in a semi-solid culture medium

Apesar da Tricomoníase ser, geral, a doença sexualmente transmitida mais frequentemente diagnosticada, existem poucas informaçöes relacionadas a padronizaçäo de cultivo do agente etiológico em meio semi-sólido. No presente trabalho, 10 estirpes de Trichomonas vaginalis foram semeadas em meio semi...

Glicosaminoglicanos na parede da aorta de pacientes submetidos à cirurgia de revascularizaçäo do miocardio: suas relaçöes com variaveis do perfil lipídico sangüíneo
Glycosaminoglycans in the aortic wall of patients subjected to myocardial revascularization: its relationship with variations of serum lipids profile

Arq. bras. cardiol; 49 (5), 1987
Inexistindo investigaçöes no homen (in vivo), sobre eventuais relaçöes entre o GAG (glicosaminoglicanos) da parede arterial e a variaçäo das fraçöes lipídicas do sangue circulante, a presente pesquisa foi planejada utilizando fragmentos da aorta obtidos na abertura do óstio para implante da vei...

Patrón electroforético anómalo de la deshidrogenasa láctica por fijación a complejo con IgA
Lactic dehydrogenase anomalous electrophoretic pattern due by complex fixation to IgA

Se presenta el caso de un patrón anormal en el isoenzimograma de la deshidrogenasa láctica (DHL), caracterizado por la ausencia de la banda dos y una hiperactividad en la zona de la banda tres; otras zonas mostraron actividad normal (electroforesis en agarosa). La paciente, con artritis psoriática, co...