Introducción. En 1997, en el departamento de Tarija, Bolivia, situado en la frontera con Argentina, se notificó por primera vez la presencia de pacientes con úlceras en las partes descubiertas de la piel, cuyas características clínicas y epidemiológicas correspondían a leishmaniasis. Objetivo. Des...
Abstract INTRODUCTION: In the Belém Metropolitan Region (BMR), Pará State, Brazil, American cutaneous leishmaniasis (ACL) is endemic; however, very little is known regarding its causative agents. Therefore, we used our standard diagnostic approach combined with an RNA polymerase II largest subunit (RN...
Côrtes, Luzia Monteiro de Castro;
de Pita-Pereira, Daniela;
Farani, Priscila Silva Grijó;
Pereira, Bernardo Acácio Santini;
Dias-Lopes, Geovane;
da Silva, Franklin Souza;
Corrêa, Paloma Resende;
Silva, Roger Magno Macedo;
Côrte-Real, Suzana;
Bello, Felio Jesus;
Mendonça-Lima, Leila;
Moreira, Otacilio da Cruz;
Waghabi, Mariana Caldas;
Alves, Carlos Roberto.
BACKGROUND Lutzomyia longipalpis-derived cell line (Lulo) has been suggested as a model for studies of interaction between sandflies and Leishmania. OBJECTIVES Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with Leishmania (Viannia) braziliensis. M...
BACKGROUND Oxidative stress is responsible for generating DNA lesions and the 8-oxoguanine (8-oxoG) is the most commonly lesion found in DNA damage. When this base is incorporated during DNA replication, it could generate double-strand DNA breaks and cellular death. MutT enzyme hydrolyzes the 8-oxoG fro...
Antimonio/toxicidad,
Antiprotozoarios/farmacología,
Proteínas de Escherichia coli/genética,
Proteínas de Escherichia coli/metabolismo,
Escherichia coli,
Guanina/análogos & derivados,
Guanina/farmacología,
Peróxido de Hidrógeno/toxicidad,
Leishmania braziliensis/efectos de los fármacos,
Leishmania braziliensis/enzimología,
Leishmania infantum/efectos de los fármacos,
Leishmania infantum/enzimología,
Estrés Oxidativo/efectos de los fármacos,
Estrés Oxidativo/fisiología,
Pirofosfatasas/genética,
Pirofosfatasas/metabolismo,
Conejos,
Superóxido Dismutasa/genética,
Superóxido Dismutasa/metabolismo
BACKGROUND Metformin (MET) is a hypoglycemic drug used for the treatment of diabetes, despite interference in host immunity against microorganisms. Cutaneous infection caused by pathogens such as Leishmania braziliensis (Lb), the agent responsible for cutaneous leishmaniasis (CL) in Brazil, represents a...
BACKGROUND The leishmaniases are complex neglected diseases caused by protozoan parasites of the genus Leishmania. Leishmania braziliensis is the main etiological agent of cutaneous leishmaniasis in the New World. In recent studies, genomic changes such as chromosome and gene copy number variations (CNV...
BACKGROUND The mechanism of resistance to SbIII in Leishmania is complex, multifactorial and involves not only biochemical mechanisms, but also other elements, such as the immune system of the host. OBJECTIVES In this study, putative changes in the immunological profile of human monocytes infected wit...
Antimonio/farmacología,
Antiprotozoarios/farmacología,
Resistencia a Medicamentos,
Citometría de Flujo,
Inmunidad Innata,
Leishmania braziliensis/efectos de los fármacos,
Leishmania braziliensis/inmunología,
Leishmania infantum/efectos de los fármacos,
Leishmania infantum/inmunología,
Monocitos/inmunología,
Monocitos/parasitología,
Óxido Nítrico/biosíntesis,
Fagocitosis/inmunología
Abstract Blood samples and swabs from ocular conjunctiva and mouth were obtained from 64 cats. Of 64 serum samples, 19 were positive for Leishmania antibodies by ELISA (29.80%). Eight cats were positive by PCR (12.5%) in swab samples from mouth and/or ocular mucosa. Poor kappa agreement between serologic...
Anticuerpos Antiprotozoarios/sangre,
Enfermedades de los Gatos/diagnóstico,
Enfermedades de los Gatos/parasitología,
ADN Protozoario/análisis,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania braziliensis/genética,
Leishmania braziliensis/inmunología,
Leishmania braziliensis/aislamiento & purificación,
Leishmania infantum/genética,
Leishmania infantum/inmunología,
Leishmania infantum/aislamiento & purificación,
Leishmaniasis/diagnóstico,
Leishmaniasis/parasitología,
Filogenia,
Reacción en Cadena de la Polimerasa/veterinaria
Objetivo: Realizar uma análise histopatológica e molecular em biópsia de pele entre as lesões de dermatites de pacientes com suspeita de Leishmaniose Tegumentar Americana (LTA) no hospital de referência do estado de Pernambuco entre o período de 2016 e 2017. Métodos: Trata-se de um estudo descriti...
La leishmaniasis cutánea se caracteriza por la inducción de una respuesta inmune celular y humoral, siendo los niveles de anticuerpos IgG e IgE indicadores serológicos del diagnóstico, la progresión y resolución de la enfermedad. En el presente estudio se estandarizaron dos pruebas de ELISA indirec...
