Home > LILACS - Literatura Latinoamericana y del Caribe de Ciencias de la Salud > Search result

Results: 68

A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model

Souza, T F G; Pierdoná, T M; Macedo, F S; Aquino, P E A; Rangel, G F P; Duarte, R S; Silva, L M A; Viana, G S B; Alves, A P N N; Montenegro, R C; Wilke, D V; Silveira, E R; Alencar, N M N.
Braz. j. med. biol. res; 54 (9), 2021
It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. Ha...
Quemaduras/tratamiento farmacológico, Queratinocitos, Metanol, Hojas de la Planta, Prolina, Sapotaceae
Fulltext

In vitro haemostatic efficacy of aqueous, methanol and ethanol plant extracts of three medicinal plant species in Palestine

Omar, G; Abdallah, L; Barakat, A; Othman, R; Bourinee, H.
Braz. j. biol; 80 (4), 2020
Abstract The haemostatic efficacy of different extract types of Satureja thymbra L., Thymbra spicata L. (Lamiaceae) and Verbascum fruticulosum Post. (Scrophulariaceae) was evaluated in this study via the Prothrombin time (PT) and Activated partial thromboplastin time (aPTT) analysis. Aqueous, methanol an...
Etanol, Hemostasis, Hemostáticos, Metanol, Extractos Vegetales/farmacología, Hojas de la Planta, Plantas Medicinales
Fulltext

Comparison of protein precipitation methods for sample preparation prior to proteomic analysis of Chinese hamster ovary cell homogenates

Pérez-Rodriguez, Saumel; Ramírez, Octavio T; Trujillo-Roldán, Mauricio A; Valdez-Cruz, Norma A.
Electron. j. biotechnol; 48 (), 2020
BACKGROUND: Chinese hamster ovary (CHO) cells are the workhorse for obtaining recombinant proteins. Proteomic studies of these cells intend to understand cell biology and obtain more productive and robust cell lines for therapeutic protein production in the pharmaceutical industry. Because of the great i...
Proteínas Recombinantes, Células CHO, Precipitación Química, Proteómica/métodos, Separación Celular, Solubilidad, Electroforesis en Gel de Poliacrilamida, Técnicas de Cultivo de Célula, Acetona, Cloroformo, Metanol, Ácido Tricloroacético
Fulltext

Alcohol-based fixatives can better preserve tissue morphology than formalin

Haque, Ziaul; Rahman, Asabur; Khan, Zahirul Islam; Hussan, Mohammad Tufazzal; Alam, Mahmudul.
Int. j. morphol; 38 (5), 2020
SUMMARY: Fixation is a crucial step in processing of tissue specimen for preservation of cellular architecture and composition of cells. Alcohol-based fixatives are considered some of the most promising alternatives to formalin. We evaluated the performance of alcohol-based fixatives (EthMeth and methaca...
Fijación del Tejido/métodos, Fijadores, Alcoholes/química, Formaldehído/química, Ácido Acético/química, Cloroformo/química, Metanol/química
Fulltext

Release of formaldehyde during the biofiltration of methanol vapors in a peat biofilter inoculated with Pichia pastoris GS115

Guerrero, Karlo; Arancibia, Alejandra; Caceres, Manuel; Aroca, German.
Electron. j. biotechnol; 44 (), 2020
BACKGROUND: Methanol can be effectively removed from air by biofiltration (Shareefdeen et al., 1993; Babbitt et al., 2009 [1,2]). However, formaldehyde is one of the first metabolic intermediates in the consumption of methanol in methylotrophic microorganisms (Negruţa et al., 2010 [3]), and it can be re...
Pichia/química, Filtros Biológicos, Formaldehído/análisis, Volatilización, Metanol/química, Biomasa, Ambiente, Reactores Biológicos
Fulltext

Phenolic profile and antioxidant, acetylcholinesterase, and tyrosinase inhibitor activities of Jurinea tzar-ferdinandii Davidov, an endemic plant of the Balkans

Trendafilova, Antoaneta; Kutova, Nikolina; Ozek, Gulmira; Todorova, Milka; Yur, Suleyman; Ozek, Temel; Dimitrov, Dimitar.
Bol. latinoam. Caribe plantas med. aromát; 19 (2), 2020
The methanol extract of the Balkan endemic species Jurinea tzar-ferdinandii Davidov demonstrated weak antioxidant activity against DPPH• and ABTS+• and low inhibitory potential against acetylcholinesterase (8.3% Inh.) and tyrosinase (IC50 = 208 ± 8 µg/mL) enzymes. Phytochemical investigation of the...
Asteraceae/química, Antioxidantes/farmacología, Antioxidantes/química, Flavonoides/análisis, Inhibidores de la Colinesterasa, Monofenol Monooxigenasa/antagonistas & inhibidores, Fenoles/análisis, Metanol, Peninsula Balcánica
Fulltext

Release of formaldehyde during the biofiltration of methanol vapors in a peat biofilter inoculated with Pichia pastoris GS115

Guerrero, Karlo; Arancibia, Alejandra; Caceres, Manuel; Aroca, German.
Electron. j. biotechnol; 40 (), 2019
Background: Methanol can be effectively removed from air by biofiltration. However, formaldehyde is one of the first metabolic intermediates in the consumption of methanol in methylotrophic microorganisms, and it can be released out of the cell constituting a secondary emission. Results: The total remova...
Pichia/metabolismo, Formaldehído/metabolismo, Metanol/metabolismo, Filtración, Contaminantes Atmosféricos, Biomasa, Ambiente
Fulltext

Evaluation of smooth muscle relaxant potential of Bismarckia nobilis (Hildebr. & Wendl.) in diarrhea, hypertension and asthma by ex-vivo and in-vivo method

Saqib, Fatima; Jabeen, Naila; Riaz, Muhammad; Sechel, Gabriela.
Bol. latinoam. Caribe plantas med. aromát; 18 (2), 2019
To explore the mechanistic basis behind smooth muscle relaxant prospective of Bismarckia nobilis in gastrointestinal, respiratory and cardiovascular ailments. The methanolic extract of B. nobilis and sub-fractions have been evaluated in vitro rabbit isolated tissues, in vivo castor oil-induced diarrhea i...
Arecaceae, Extractos Vegetales/farmacología, Metanol, Antiasmáticos/farmacología, Antidiarreicos/farmacología, Antihipertensivos/farmacología, Bloqueadores de los Canales de Calcio/farmacología, Relajación Muscular/efectos de los fármacos, Músculo Liso/efectos de los fármacos, Aorta/efectos de los fármacos, Yeyuno/efectos de los fármacos, Tráquea/efectos de los fármacos, Hipotensión/metabolismo, Diarrea/metabolismo, Asma/metabolismo
Fulltext