Abstract INTRODUCTION: Mycobacterium tuberculosis (MTB) is a causative agent of tuberculosis (TB) that causes death worldwide. METHODS: MTB was subjected to phenotypic drug-susceptibility tests (DST), and drug-resistant genes were sequenced. RESULTS: Previously treated patients were more likely to hav...
ABSTRACT Tuberculosis verrucosa cutis is a rare medical condition that is caused by the inoculation of Mycobacterium tuberculosis into the skin of a previously sensitized individual. This clinical form of tuberculosis corresponds to 1-2% of all cases of tuberculosis and due to the paucibacillary characte...
ABSTRACT Background: The timely diagnosis of tuberculous meningitis (TBM) is challenging. Molecular diagnostic tools are necessary for TBM, particularly in low- and middle-income countries. Objectives: We aimed to calculate the diagnostics characteristics of Xpert MTB/RIF for the detection of Mycobacte...
Abstract Tuberculosis (TB) is one of the infectious diseases with high mortality in the world. DNA amplification techniques have been used to overcome barriers to the diagnosis of this disease. However, the success of these methodologies is highly dependent on the DNA obtained from the sample. This study...
Guimarães, Arthur Emil dos Santos;
Sharma, Abhinav;
Furlaneto, Ismari Perini;
Rutaihwa, Liliana;
Cardoso, Jedson Ferreira;
da Conceição, Marília Lima;
Spinassé, Lizânia Borges;
Machado, Edson;
Lopes, Maria Luiza;
Duarte, Rafael Silva;
Gagneux, Sebastien;
Suffys, Philip Noel;
Lima, Karla Valéria Batista;
Conceição, Emilyn Costa.
BACKGROUND The evaluation of procedures for drug susceptibility prediction of Mycobacterium tuberculosis based on genomic data against the conventional reference method test based on culture is realistic considering the scenario of growing number of tools proposals based on whole-genome sequences (WGS)....
Antituberculosos/farmacología,
Antituberculosos/uso terapéutico,
Brasil,
Farmacorresistencia Bacteriana Múltiple/efectos de los fármacos,
Farmacorresistencia Bacteriana Múltiple/genética,
Pruebas de Sensibilidad Microbiana,
Mycobacterium tuberculosis/efectos de los fármacos,
Mycobacterium tuberculosis/genética,
Mycobacterium tuberculosis/aislamiento & purificación,
Preparaciones Farmacéuticas,
Tuberculosis Resistente a Múltiples Medicamentos/tratamiento farmacológico,
Tuberculosis Resistente a Múltiples Medicamentos/genética,
Secuenciación Completa del Genoma
Abstract INTRODUCTION: Laboratory and clinical features of childhood tuberculosis (TB) are non-specific and establishing an accurate diagnosis remains a challenge. This study evaluated a Single tube nested-PCR (STNPCR) to detect genomic DNA of Mycobacterium tuberculosis complex in blood and urine. METH...
Abstract INTRODUCTION: Tuberculosis is listed among the top 10 causes of deaths worldwide. The resistant strains causing this disease have been considered to be responsible for public health emergencies and health security threats. As stated by the World Health Organization (WHO), around 558,000 differe...
Abstract INTRODUCTION: Nontuberculous mycobacteria (NTM) species, as human pathogens, are increasing in the world, as is the difficulty of accurately identifying them. Differential diagnosis, especially between the M. tuberculosis complex and NTM species, and the characterization of NTM species is impo...
Abstract INTRODUCTION: The diagnostic accuracy of Xpert MTB/RIF (Xpert) in pulmonary tuberculosis (PTB) in children is lower than in adults. In Brazil, the diagnosis of PTB is based on a diagnostic score system (DSS). This study aims to study the role of Xpert in children and adolescents with PTB sympt...
The human-adapted strains of the Mycobacterium tuberculosis complex (MTBC) comprise seven phylogenetic lineages originally associated with their geographical distribution. Here, we report the genomes of three drug-resistant clinical isolates of the Latin American-Mediterranean (LAM) family collected in K...
