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High-yield production of the human lysozyme by Pichia pastoris SMD1168 using response surface methodology and high-cell-density fermentation

Yu, Ying; Zhou, Xiaoyu; Wu, Sheng; Wei, Tiantian; Yu, Long.
Electron. j. biotechnol; 17 (6), 2014
Background Lysozyme plays a crucial role in innate immunity with its well-recognized bacteriolytic activity. In this study, the influence of expression parameters (inoculation volume, culture volume, growth time, induction temperature and time, initial pH and methanol concentration) on human lysozyme (HL...
Muramidasa/metabolismo, Pichia/metabolismo, Fermentación, Muramidasa/biosíntesis, Técnicas de Cultivo Celular por Lotes, Análisis de Varianza, Reactores Biológicos, Concentración de Iones de Hidrógeno, Temperatura
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Elevating the expression level of biologically active recombinant human alpha 1-antitrypsin in Pichia pastoris

Arjmand, Sareh; Lotfi, Abbas Sahebghadam; Shamsara, Mehdi; Mowla, Seyed Javad.
Electron. j. biotechnol; 16 (1), 2013
Background: Human alpha 1-antitrypsin (AAT) is a potent inhibitor of multiple serine proteases, and protects tissues against their harmful effects. Individuals with reduced or abnormal production of this inhibitor need intravenous administration of exogenous protein. In this study, we employed the methyl...
Pichia/metabolismo, Proteínas Recombinantes/metabolismo, alfa 1-Antitripsina/metabolismo, ADN/aislamiento & purificación, Pichia/genética, Transformación Genética, Reacción en Cadena en Tiempo Real de la Polimerasa, ADN Complementario, Ensayo de Inmunoadsorción Enzimática, Inhibidores Enzimáticos
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