LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro

Bastos, Matheus Silva e; Souza, Luciana Ângelo de; Onofre, Thiago Souza; Silva Júnior, Abelardo; Almeida, Márcia Rogéria de; Bressan, Gustavo Costa; Fietto, Juliana Lopes Rangel.

Mem. Inst. Oswaldo Cruz; 112 (2), 2017

BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS L. braziliensis-GFP was generated using Leishmania...

Evaluación Preclínica de Medicamentos/métodos, Antiprotozoarios/farmacología, Anfotericina B/farmacología, Proteínas Fluorescentes Verdes/farmacología, Leishmania braziliensis/efectos de los fármacos, Microscopía Fluorescente, Factores de Tiempo

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Processos de produção da proteína heteróloga mCherry por Chlamydomonas reinhardtii

Diaz Arias, Cesar Andres.

Este trabalho tem como finalidade estudar as melhores condições do cultivo da microalga Chlamydomonas reindhartii geneticamente modificada para a produção da proteína fluorescente mCherry a partir do estudo dos macronutrientes contidos no meio de cultivo. A proteína mCherry possui a vantagem de ser...

Chlamydomonas reinhardtii/crecimiento & desarrollo, Proteínas Fluorescentes Verdes/análisis, Microalgas/crecimiento & desarrollo, Fotobiorreactores/22074, Nitrógeno

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Establishment of a HEK293T cell line able to site-specifically integrate and stably express GDNF by rAAV-2 vector

Zhang, Jinju; Zhang, Yun; Liu, Xiaomei; Xiang, Jingjing; Zhang, Chun.

Electron. j. biotechnol; 19 (4), 2016

Background: Using recombinant adeno-associated virus 2 (rAAV-2), we attempted to establish a HEK293T cell line that is able to site-specifically integrate and stably express glial cell line-derived neurotrophic factor (GDNF). Results:Recombinant vector with enhanced green fluorescent protein (EGFP) and G...

Células HEK293, Línea Celular, Transducción Genética, Recombinación Genética, Dependovirus, Factor Neurotrófico Derivado de la Línea Celular Glial, Reacción en Cadena de la Polimerasa, Vectores Genéticos, Microscopía Fluorescente, Proteínas Fluorescentes Verdes

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Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using a green fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy

Figueiredo, Márcio de Azevedo; Lanes, Carlos Frederico Ceccon; Almeida, Daniela Volcan; Marins, Luis Fernando.

Genet. mol. biol; 30 (1), 2007

In fish, microinjection is the method most frequently used for gene transfer. However, due to delayed transgene integration this technique almost invariably produces mosaic individuals and if the gene is not integrated into germ cells its transmission to descendants is difficult or impossible. We evaluat...

Pez Cebra/genética, Animales Modificados Genéticamente/crecimiento & desarrollo, Proteínas Fluorescentes Verdes, Microinyecciones, Hormona del Crecimiento, ADN Complementario

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