Frente a la creciente demanda de producción de la vacuna Antihepatitis B recombinante, constituye un reto para el Centro Nacional de Biopreparados aumentar la fabricación del producto, para lo cual el proceso de llenado aséptico requirió de una inversión. En el trabajo se presenta la selección de l...
The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors. Based on cloned new plasmids pPIC3.5-PreS2-S (8707 bp) and pPIC9-PreS2-S (8980 bp)...
BACKGROUND: Cecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have show...
Cunha, Rodrigo Casquero;
Saimo-Kahwa, Margaret;
Garcia, Marcos Valério;
Santos, Francisco Denis Souza;
Samuel, Emukule;
Ann, Nanteza;
Ikwap, Kokas;
Akwongo, Claire Julie;
Leite, Fábio Pereira Leivas;
Andreotti, Renato.
In this study, the recombinant gut protein rRa92A produced in Pichia pastoris yeast cells was used to immunize cattle in two experiments, one in Brazil and the other in Uganda. In both experiments, the animals were intramuscularly (IM) injected with 200 µg of recombinant protein in Brazil on days 0, 30 ...
BACKGROUND: Milk whey, a byproduct of the dairy industry has a negative environmental impact, can be used as a raw material for added-value compounds such as galactooligosaccharides (GOS) synthesis by bgalactosidases. RESULTS: B-gal42 from Pantoea anthophila strain isolated from tejuino belonging to the ...
Em geral, a doença causada pelo vírus Zika (ZIKV) é clinicamente semelhante às de outros arbovírus. No entanto, ocasionalmente ela é associada a síndromes neurológicas como a síndrome de Guillain-Barré. Além disso, durante a gravidez a infecção pode causar a síndrome congênita do zika, com...
Silva, Laís Teodoro da;
Ortega, Marina Mazzilli;
Tiyo, Bruna Tiaki;
Viana, Isabelle Freire Tabosa;
Lima, Tayná Evily de;
Tozetto-Mendoza, Tania Regina;
Oliveira, Luanda Mara da Silva;
Teixeira, Franciane Mouradian Emidio;
Lins, Roberto Dias;
Almeida, Alexandre de;
Mendes-Correa, Maria Cassia;
Duarte, Alberto Jose da Silva;
Oshiro, Telma Miyuki.
OBJECTIVES: In this preliminary study we investigated cellular and humoral immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in blood samples from 14 recovered coronavirus disease 2019 (COVID-19) patients and compared them to those in samples from 12 uninfected/unv...
Fujimori, Mahyumi;
de Almeida, Arleana do Bom Parto Ferreira;
Barrouin-Melo, Stella Maria;
Cortez, Luiz Ricardo Paes de Barros;
Duthie, Malcolm Scott;
Hiramoto, Roberto Mitsuyoshi;
de Pinho, Flaviane Alves;
Reed, Steven Gregory;
Sousa, Valéria Régia Franco;
Souza, Nazaré Fonseca;
Soares, Rodrigo Martins;
Tolezano, José Eduardo;
Sanchez, Maria Carmen Arroyo;
Goto, Hiro.
BACKGROUND Dogs are the main peridomiciliary reservoir of Leishmania infantum thus the correct diagnosis of infection is essential for the control of the transmission and treatment as well. However, the diagnosis is based on serological assays that are not fully effective. OBJECTIVE We aimed to establ...
Antígenos de Protozoos/biosíntesis,
Anticuerpos Antiprotozoarios/sangre,
Brasil,
Enfermedades de los Perros/diagnóstico,
Ensayo de Inmunoadsorción Enzimática/métodos,
Ensayo de Inmunoadsorción Enzimática/normas,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania infantum/inmunología,
Leishmania infantum/aislamiento & purificación,
Leishmaniasis Visceral/sangre,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/veterinaria,
Proteínas Recombinantes/inmunología,
Sensibilidad y Especificidad,
Pruebas Serológicas
Abstract Leptospirosis is a wide spread bacterial zoonosis that is common worldwide. The disease symptoms are mild or acute. Leptospira has pathogenic and non-pathogenic species; it has a lot of surface antigens. Adenylate Guanylate Cyclase (AGC) is a membrane protein that is found only in pathogenic spe...
Abstract Objective This study sought to compare the biocompatibility of a three-dimensional (3D)-printed titanium implant with a conventional machined titanium product, as well as the effect of such implant applied with recombinant human Bone Morphogenetic Protein Type 2 (rhBMP-2) for guided bone regen...
