Resumen Introducción: Las Enterobacteriaceae productoras de carbapenemasas (EPC) han tomado gran importancia en salud pública a una escala global, haciendo necesario implementar test rápidos para su detección oportuna. Objetivo: Evaluar tres metodologías para el tamizaje de EPC en hisopados rectal...
ABSTRACT Objective To compare the performance of the Ogawa-Kudoh method with the modified Petroff technique in diagnosis of pulmonary tuberculosis. Methods A total of 205 sputum samples from 166 patients with clinical suspicion or under pulmonary tuberculosis follow-up, seen at a public tertiary care...
ABSTRACT Objective To evaluate the incidence of variant hemoglobins in different health regions. Methods A descriptive, observational, and cross-sectional study with a quantitative approach based on secondary data in the internal records of the neonatal screening service - Laboratório Central de Saúd...
ABSTRACT Rapid identification of vancomycin-resistant enterococci (VRE) can assist in choosing the appropriate treatment and preventing VRE spread. The performance of chromIDTM VRE agar was evaluated using 184 clinical isolates of Enterococcus spp. and reference strains. The test had a sensitivity of 95....
Dantas, Nayanne Gama Teixeira;
Suffys, Phillip Noel;
Carvalho, Wânia da Silva;
Gomes, Harrison Magdinier;
Almeida, Isabela Neves de;
Figueiredo, Lida Jouca de Assis;
Gonçalves, Alan Douglas;
Gomgnimbou, Michel Kireopori;
Refregier, Guislaine;
Sola, Christophe;
Miranda, Silvana Spíndola de.
BACKGROUND The accurate detection of multidrug-resistant tuberculosis (MDR-TB) is critical for the application of appropriate patient treatment and prevention of transmission of drug-resistant Mycobacterium tuberculosis isolates. The goal of this study was to evaluate the correlation between phenotypic a...
Resumen La tuberculosis (TBC) extra-pulmonar alcanza al 26,2% de los casos totales de TBC en Chile. El cultivo es el método estándar de oro, pero es lento. La técnica Xpert® MTB/RIF permite detectar Mycobacterium tuberculosis complex (MTBc) por RPC en tiempo real en menos de 3 h, sin embargo, ha sido...
Abstract A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Usi...
Abstract Our aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo® Bacterial Meningitis Test (SO-BMT) – a hybridization-based molecular test method – during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningi...
Técnicas Bacteriológicas/métodos,
Pruebas Diagnósticas de Rutina/métodos,
Meningitis Bacterianas/diagnóstico,
Técnicas de Diagnóstico Molecular/métodos,
Neisseria meningitidis/aislamiento & purificación,
Reacción en Cadena de la Polimerasa/métodos,
Streptococcus pneumoniae/aislamiento & purificación,
Líquido Cefalorraquídeo/microbiología,
Neisseria meningitidis/clasificación,
Neisseria meningitidis/genética,
Neisseria meningitidis/crecimiento & desarrollo,
Estudios Retrospectivos,
Sensibilidad y Especificidad,
Streptococcus pneumoniae/clasificación,
Streptococcus pneumoniae/genética,
Streptococcus pneumoniae/crecimiento & desarrollo
Abstract The modified Carba NP test presented here may be a valuable tool for laboratories interested in investigating a large number of carbapenemase-producing bacteria in a less-costly way. The test was evaluated against 48 carbapenemase-producing and carbapenemase-non-producing gram-negative bacteria....
BACKGROUND: Multiple techniques exist for detecting Mycobacteria, each having its own advantages and drawbacks. Among them, automated culture-based systems like the BACTEC-MGIT™ are popular because they are inexpensive, reliable and highly accurate. However, they have a relatively long "time-to-detecti...
