SUMMARY: Cell culture is an important tool in medical, odontological and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. Gingival fibroblasts present structural function, being able to modulate their metabolic capacity, which is reflected in the tissue mo...
ABSTRACT Purpose: To evaluate the ability of human immature dental pulp stem cells, which are mesenchymal stem cells of neural crest origin, to differentiate into the corneal epithelium for purposes of corneal transplantation and tissue engineering when cultured on de-epithelized amniotic membranes. Me...
Amnios,
Técnicas de Cultivo de Célula/métodos,
Proliferación Celular,
Células Cultivadas,
Enfermedades de la Córnea/cirugía,
Pulpa Dental/citología,
Epitelio Corneal/trasplante,
Técnica del Anticuerpo Fluorescente,
Trasplante de Células Madre Mesenquimatosas/métodos,
Células Madre Mesenquimatosas/citología,
Reproducibilidad de los Resultados,
Factores de Tiempo,
Andamios del Tejido
SUMMARY: Matrigel is a basement membrane matrix extracted from the EHS mouse tumor containing extracellular matrix protein, its main components are laminin, type IV collagen, nestin, heparin sulfate, growth factor and matrix metalloproteinase.At room temperature, Matrigel polymerized to form a three dime...
Abstract Cementum is the mineralized tissue covering the tooth root that functions in tooth attachment and post-eruptive adjustment of tooth position. It has been reported to be highly similar to bone in several respects but remains poorly understood in terms of development and regeneration. Here, we inv...
Proteínas Adaptadoras Transductoras de Señales,
Análisis de Varianza,
Proteínas Morfogenéticas Óseas/análisis,
Proteínas Morfogenéticas Óseas/genética,
Técnicas de Cultivo de Célula/métodos,
Línea Celular,
Cemento Dental/metabolismo,
Proteínas de la Matriz Extracelular/análisis,
Proteínas de la Matriz Extracelular/genética,
Técnica del Anticuerpo Fluorescente,
Expresión Génica,
Marcadores Genéticos/genética,
Glicoproteínas de Membrana/análisis,
Glicoproteínas de Membrana/genética,
Diente Molar/citología,
Fosfatos/farmacología,
Fosfoproteínas/análisis,
Fosfoproteínas/genética,
Sialoglicoproteínas/análisis,
Sialoglicoproteínas/genética,
Factores de Tiempo,
Cemento Dental/citología
ABSTRACT Purposes: To develop an efficient and xeno-free standard eye-derived induced pluripotent stem cell reprogramming protocol for use during induced pluripotent stem cell-based cell therapies in treating retinal degenerative diseases and to compare the relative effectiveness of both animal- and non...
Abstract Recently, human natal dental pulp stem cells (hNDP-SCs) have been characterized in vitro and it has been shown that they satisfy criteria defining human mesenchymal stromal cells (MSCs), as proposed by the International Society for Cellular Therapy. However, these results were reached in the pre...
Técnicas de Cultivo de Célula/métodos,
Diferenciación Celular,
Proliferación Celular,
Supervivencia Celular,
Células Cultivadas,
Pulpa Dental/citología,
Dientes Neonatales/citología,
Fenotipo,
Plasma Rico en Plaquetas,
Células Madre/citología,
Acortamiento del Telómero
ABSTRACT Objectives: The present review intended to present a critical overview of the methodological and experimental advances concerning tridimensional cell culture models within the scope of gastrointestinal research. Methods: A literature review was performed and some of the main published articles...
Abstract INTRODUCTION: Rabies is an acute zoonotic disease, caused by a rhabdovirus that can affect all mammals, and is commonly transmitted by the bite of a rabid animal. The definitive diagnosis is laboratorial, by the Fluorescent Antibody Test (FAT) as a quick test and Mouse Inoculation Test (MIT) a...
Bienestar del Animal,
Discusiones Bioéticas,
Técnicas de Cultivo de Célula/economía,
Técnicas de Cultivo de Célula/métodos,
Contención de Riesgos Biológicos,
Costos y Análisis de Costo,
Técnica del Anticuerpo Fluorescente/economía,
Técnica del Anticuerpo Fluorescente/métodos,
Ratones,
Salud Laboral,
Rabia/diagnóstico,
Virus de la Rabia/inmunología,
Virus de la Rabia/aislamiento & purificación,
Reproducibilidad de los Resultados,
Factores de Riesgo,
Sensibilidad y Especificidad
O câncer é composto pelas células malignas em proliferação associadas às diferentes células circunjacentes, formando o microambiente tumoral (TME), onde há uma constante troca de informações. Uma das formas de comunicação entre os diferentes tipos celulares do TME se dá por meio da liberaç�...
Carcinoma de Células Escamosas de Cabeza y Cuello/patología,
Microambiente Tumoral,
Técnicas de Cultivo de Célula/métodos,
Vesículas Extracelulares,
Macrófagos Asociados a Tumores,
28573,
Análisis de Varianza,
Estadísticas no Paramétricas,
Ultracentrifugación,
Antígeno Ki-67,
Técnicas In Vitro/métodos
