Results: 3204

Sugarcane straw as a feedstock for xylitol production by Candida guilliermondii FTI 20037

Braz. j. microbiol; 47 (2), 2016
Abstract Sugarcane straw has become an available lignocellulosic biomass since the progressive introduction of the non-burning harvest in Brazil. Besides keeping this biomass in the field, it can be used as a feedstock in thermochemical or biochemical conversion processes. This makes feasible its incorpo...

Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR

Braz. j. microbiol; 47 (2), 2016
Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samp...

Isolation and expression of two polyketide synthase genes from Trichoderma harzianum 88 during mycoparasitism

Braz. j. microbiol; 47 (2), 2016
Abstract Metabolites of mycoparasitic fungal species such as Trichoderma harzianum 88 have important biological roles. In this study, two new ketoacyl synthase (KS) fragments were isolated from cultured Trichoderma harzianum 88 mycelia using degenerate primers and analysed using a phylogenetic tree. The ...

Occurrence and characteristics of extended spectrum beta-lactamases-producing Enterobacteriaceae from foods of animal origin

Braz. j. microbiol; 47 (2), 2016
Abstract Presence of extended spectrum beta-lactamases (ESBL) in bacteria is a growing health concern of global significance. The local, regional, national, and international epidemiological studies for extended spectrum beta-lactamases-producing Enterobacteriaceae and their encoding genes in foods are s...

Curli fimbria: an Escherichia coli adhesin associated with human cystitis

Braz. j. microbiol; 47 (2), 2016
Abstract Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence o...

A protein expression system for tandem affinity purification in Xanthomonas citri subsp. citri

Braz. j. microbiol; 47 (2), 2016
Abstract Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp. citri (Xac), is one of the most devastating diseases to affect citrus crops. There is no treatment for citrus canker; effective control against the spread of Xac is usually achieved by the elimination of affected plant...

Rhizobial characterization in revegetated areas after bauxite mining

Braz. j. microbiol; 47 (2), 2016
Abstract Little is known regarding how the increased diversity of nitrogen-fixing bacteria contributes to the productivity and diversity of plants in complex communities. However, some authors have shown that the presence of a diverse group of nodulating bacteria is required for different plant species t...

Cellulase activity and dissolved organic carbon release from lignocellulose macrophyte-derived in four trophic conditions

Braz. j. microbiol; 47 (2), 2016
Abstract Considering the importance of lignocellulose macrophyte-derived for the energy flux in aquatic ecosystems and the nutrient concentrations as a function of force which influences the decomposition process, this study aims to relate the enzymatic activity and lignocellulose hydrolysis in different...

Endophytic fungus strain 28 isolated from Houttuynia cordata possesses wide-spectrum antifungal activity

Braz. j. microbiol; 47 (2), 2016
Abstract The aim of this paper is to identify and investigate an endophytic fungus (strain 28) that was isolated from Houttuynia cordata Thunb, a famous and widely-used Traditional Chinese Medicine. Based on morphological methods and a phylogenetic analysis of ITS sequences, this strain was identified as...

Characterization of the variable region in the class 1 integron of antimicrobial-resistant Escherichia coli isolated from surface water

Braz. j. microbiol; 47 (2), 2016
Abstract Fecal bacteria are considered to be a potential reservoir of antimicrobial resistance genes in the aquatic environment and could horizontally transfer these genes to autochthonous bacteria when carried on transferable and/or mobile genetic elements. Such circulation of resistance genes constitut...