Results: 354

Utility of multiplex real-time PCR in the diagnosis of extrapulmonary tuberculosis

Braz. j. infect. dis; 20 (3), 2016
Abstract Objective The diagnosis of extrapulmonary tuberculosis is still a challenge because of its pauci-bacillary nature. The aim of the study was to evaluate the role of a multiplex PCR assay in the diagnosis of extrapulmonary tuberculosis and to compare the efficiency of two targets, IS6110 and MPB6...

Importancia del control de la calidad de la baciloscopia en los laboratorios de diagnóstico de tuberculosis

Rev. chil. infectol; 33 (3), 2016
Introducción: La baciloscopia es la herramienta primaria en el diagnóstico de la tuberculosis (TBC) pulmonar activa, siendo esta la técnica más utilizada internacionalmente en la búsqueda de casos infecciosos. El control de calidad consiste en la relectura de las láminas por un observador altamente...

Zoonotic tuberculosis: A comprehensive one health approach

Medicina (B.Aires); 76 (3), 2016
The objective of this report is to provide information on Mycobacterium tuberculosis complex infections in animals and in humans. Included is information on the susceptibility of different species as well as information on etiology, epidemiology, pathogenesis, diagnosis, prevention and control of this di...

Análisis genómico comparativo de cepas peruanas de Mycobacterium tuberculosis

RESUMEN Objetivos. Analizar comparativamente tres secuencias genómicas de Mycobacterium tuberculosis(MTB): INS-SEN,cepa sensible; INS-MDR, cepa multidrogorresistente e INS-XDR, cepa extensamente resistente, procedentes de la Ciudad de Lima, Perú. Materiales y métodos. Se identificaron los polimorf...

Molecular characterization of mycobacterium tuberculosis isolates from Tehran, Iran by restriction fragment length polymorphism analysis and spoligotyping

Abstract: INTRODUCTION Characterization of Mycobacterium tuberculosis (MTB) isolates by DNA fingerprinting has contributed to tuberculosis (TB) control. The aim of this study was to determine the genetic diversity of MTB isolates from Tehran province in Iran. METHODS MTB isolates from 60 Iranian and ...

Evaluation of the role of Mycobacterium tuberculosis pili (MTP) as an adhesin, invasin, and cytokine inducer of epithelial cells

Braz. j. infect. dis; 20 (2), 2016
Abstract This study was undertaken in order to assess the involvement of Mycobacterium tuberculosis pili (MTP) as an adhesin, invasin, and cytokine inducer in the M. tuberculosis-epithelial cell interaction. A MTP-deficient strain of M. tuberculosis demonstrated a significant reduction of 69.39% (p = 0.0...

Detection of multidrug-resistant Mycobacterium tuberculosis strains isolated in Brazil using a multimarker genetic assay for katG and rpoB genes

Braz. j. infect. dis; 20 (2), 2016
Abstract Multidrug-resistant tuberculosis (MDRTB) is a serious world health problem that limits public actions to control tuberculosis, because the most used anti-tuberculosis first-line drugs fail to stop mycobacterium spread. Consequently, a quick detection through molecular diagnosis is essential to r...

Tuberculosis pleural en un hospital del Noreste Argentino
Pleural tuberculosis in a hospital in Northeast Argentina

Medicina (B.Aires); 76 (2), 2016
La tuberculosis (TB) pleural ocupa el primer lugar dentro de las localizaciones extrapulmonares. El objetivo de este trabajo fue estimar la proporción de TB pleural entre los casos de TB y caracterizar la forma de presentación, métodos de diagnóstico y evolución de los pacientes internados en el Ser...

Evaluación del poder discriminatorio de técnicas de epidemiología molecular en aislados Venezolanos de Mycobacterium tuberculosis

Invest. clín; 57 (1), 2016
La técnica de espoligotipaje y el método de unidades repetitivas micobacterianas interdiseminadas y número variable de repetidos tándem (MIRU-VNTR) 24 loci se emplean para estudiar la epidemiología molecular de tuberculosis. En el presente trabajo evaluamos la discriminación del método MIRU-VNTR 2...

Actividad de una esfingomielinasa ácida tipo C producida por Mycobacterium tuberculosis
An acidic sphingomyelinase Type C activity from Mycobacterium tuberculosis

Rev. argent. microbiol; 48 (1), 2016
Sphingomyelinases (SMases) catalyze the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. Sphingolipids are recognized as diverse and dynamic regulators of a multitude of cellular processes mediating cell cycle control, differentiation, stress response, cell migration, adhesion, and apoptosi...