Tello, Glenda B;
Wannan, Vanesa I;
Duarte, Andrea M;
Guerra, Walter O;
Guerra, Isabel E;
Ortiz, Ana C;
Zetina, Alfonso;
Gómez, Jorge;
De León, Jorge L;
Lange, Karla J;
Matta, Vivian L.
Se determinó la respuesta inmunológica a proteínas recombinantes de Helicobacter pylori en pacientes dis-pépticos (adultos y niños), pacientes con cáncer gástrico y sus familiares asintomáticos adultos viviendo con ellos. Se utilizó la prueba recomLine® Helicobacter IgG e IgA, y con base en el ...
Inmunoglobulina A/inmunología,
Helicobacter pylori/inmunología,
Biopsia,
Inmunoglobulina A/análisis,
Inmunoglobulina G/análisis,
Neoplasias Gástricas/inmunología,
Proteínas Recombinantes/análisis,
Helicobacter pylori/patogenicidad,
Neoplasias Gástricas/patología,
Dispepsia/inmunología,
Guatemala,
Inmunoglobulina G/inmunología,
Helicobacter pylori/aislamiento & purificación,
Neoplasias Gástricas/microbiología,
Dispepsia/microbiología,
Proteínas Recombinantes/inmunología,
Dispepsia/patología
Abstract Human Embryonic Kidney 293T cells (HEK-293T) are the most common host for viral vector production and are also widely employed for recombinant protein production. These cells are typically cultured in monolayer (adherent culture) using culture medium containing fetal bovine serum (FBS), which im...
BACKGROUND: Chinese hamster ovary (CHO) cells are the workhorse for obtaining recombinant proteins. Proteomic studies of these cells intend to understand cell biology and obtain more productive and robust cell lines for therapeutic protein production in the pharmaceutical industry. Because of the great i...
Proteínas Recombinantes,
Células CHO,
Precipitación Química,
Proteómica/métodos,
Separación Celular,
Solubilidad,
Electroforesis en Gel de Poliacrilamida,
Técnicas de Cultivo de Célula,
Acetona,
Cloroformo,
Metanol,
Ácido Tricloroacético
BACKGROUND: L-tert-Leucine has been widely used in pharmaceutical, chemical, and other industries as a vital chiral intermediate. Compared with chemical methods, enzymatic methods to produce L-tert-leucine have unparalleled advantages. Previously, we found a novel leucine dehydrogenase from the halophili...
BACKGROUND: Poly-3-hydroxybutyrate (PHB) can be efficiently produced in recombinant Escherichia coli by the overexpression of an operon (NphaCAB) encoding PHB synthetase. Strain improvement is considered to be one of critical factors to lower the production cost of PHB in recombinant system. In this stud...
Hidroxibutiratos/metabolismo,
Escherichia coli/metabolismo,
Escherichia coli/genética,
Eliminación de Gen,
Proteínas Represoras/genética,
Proteínas de Unión al ARN/genética,
Proteínas de Escherichia coli/genética,
Biopolímeros/genética,
Ligasas/metabolismo,
Proteínas Recombinantes,
Ingeniería Metabólica
Dez éguas, sem raça definida, foram submetidas a avaliações ultrassonográficas durante o intervalo interovulatório, avaliando-se folículos ≥ 5mm. Cinco éguas foram tratadas com 500mg de r-bST no primeiro e no 14º dia pós-ovulação (grupo GT), e as demais com soro fisiológico (grupo GC). Qua...
El género Pseudomonas es una fuente importante de proteasas; sin embargo, su uso está restringido en la industria alimentaria. El clonaje permite aprovechar la capacidad catalítica de estas enzimas mediante su producción en microorganismos inocuos. Por otro lado, las leguminosas son fuentes ricas en ...
Insulin-like growth factor-1 (IGF-1) is regarded as a crucial clinically significant therapeutic agent against several pathological conditions. Recently, recombinant DNA (rDNA) technology has enabled the production of many drugs of rDNA-origin including IGF-1. Securing a readily available supply of IGF-1...
Rezende, A. F. S;
Brum, A. A;
Bezerra, F. S. B;
Braite, D. C;
Sá, G. L;
Thurow, H. S;
Seixas, F. K;
Azevedo, V. A. C;
Portela, R. W;
Borsuk, S.
The target cp1002_RS01850 from Corynebacterium pseudotuberculosis was used to construct a DNA and recombinant subunit vaccine against caseous lymphadenitis. Recombinant protein rCP01850 was expressed in Escherichia coli using pAE vector, and DNA vaccine was engineered with pTARGET vector. BALB/c mice wer...
Testes diagnósticos práticos, rápidos, de fácil operacionalidade e com potencial para formato point of care (POC) são necessários para a melhor eficácia do controle da TB, principalmente em populações negligenciadas ou de difícil manejo diagnóstico como a indígena. A organização mundial da ...
