Results: 33

A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis

Braz. j. microbiol; 49 (2), 2018
Abstract Identification of nonfermenting Gram-negative bacteria (NFGNB) of cystic fibrosis patients is hard and misidentification could affect clinical outcome. This study aimed to propose a scheme using polymerase chain reaction to identify NFGNB. This scheme leads to reliable identification within 3 da...

Rapid molecular test for tuberculosis: impact of its routine use at a referral hospital

J. bras. pneumol; 44 (2), 2018
ABSTRACT Objective: To evaluate the impact of the use of the molecular test for Mycobacterium tuberculosis and its resistance to rifampin (Xpert MTB/RIF), under routine conditions, at a referral hospital in the Brazilian state of Bahia. Methods: This was a descriptive study using the database of the My...

Isolation and identification of Mycobacterium bovis in bovines with positive reaction to the tuberculin test in the state of Paraíba, northeast Brazil

Arq. Inst. Biol; 85 (), 2018
In areas where human tuberculosis and bovine tuberculosis coexist, differentiation between M. bovis and M. tuberculosis is important for monitoring the spread of M. bovis among cattle and from cattle to humans. The objective of this study was to isolate and identify M. bovis in bovines with positive diag...

Comparison of culture and PCR methods in the diagnosis of bacterial meningitis

Braz. j. microbiol; 48 (2), 2017
Abstract Our aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo® Bacterial Meningitis Test (SO-BMT) – a hybridization-based molecular test method – during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningi...

Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome

Braz. j. microbiol; 48 (2), 2017
Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase c...

Impacto orçamentário da incorporação do GeneXpert MTB/RIF para o diagnóstico da tuberculose pulmonar na perspectiva do Sistema Único de Saúde, Brasil, 2013-2017

Resumo: O objetivo do estudo foi estimar o impacto orçamentário do GeneXpert MTB/RIF para o diagnóstico da tuberculose sob a perspectiva do Programa Nacional de Controle da Tuberculose, valendo-se de um modelo estático apoiado no método epidemiológico entre 2013 e 2017. Comparou-se um teste Xpert M...

Análise dos polimorfismos dos genes CP, CYBRD1, HAMP e SLC40A1 nos pacientes com porfiria cutânea tardia
Analysis of polymorphisms of the CP, CYBRD1, HAMP and SLC40A1 genes in patients with porphyria cutanea tarda

A porfiria cutânea tardia (PCT) é a mais frequente de todas as porfirias e a de maior interesse dermatológico. Resulta da redução da atividade da enzima uroporfirinogênio descarboxilase (UROD). Sua etiologia não está completamente elucidada, sendo necessários fatores ambientais e genéticos para...

Molecular diagnosis and intraspecific genetic variability of root pathogens of arid legumes in Western Rajasthan, India

Rev. biol. trop; 64 (4), 2016
Abstract:The productivity of arid legumes, such as Clusterbean (Cyamopsis tetragonoloba), Cowpea (Vigna unguiculata), Moth bean (Vigna aconitifolia) and Horse gram (Macrotyloma uniflorum), may remain stagnant over decades because of their high susceptibility to root diseases. Besides, there is a limitati...

Monitoring the emergence of HBV resistance mutations by HBV-RNA pyrosequencing

Braz. j. infect. dis; 20 (2), 2016