Liu, Haiqiong;
Liang, Qian;
Mei, Xiheng;
Li, Hekai;
Yan, Jing;
Long, Man;
Yang, Xili;
Wang, Wei;
Li, Weijie;
Chen, Aihua;
Ling, Yuanna.
Abstract Compound Danshen Dripping Pills (CDDPs) have been used in clinical treatment to protect the heart from ischemia/reperfusion (IR) injury for many years. However, the underlying mechanism implicated in the protective effects remains to be explored. Here, we determined the effects of CDDPs in Sprag...
Ecocardiografía/métodos,
d0013430,
d0172090,
Isquemia/clasificación,
Reperfusión/clasificación,
Corazón/fisiopatología,
Infarto/patología,
Microscopía Electrónica de Transmisión/métodos,
d0275610,
Daño por Reperfusión/clasificación,
Western Blotting/instrumentación
Abstract The present study investigated the effects of valerian methanolic extract and valerenic acid on the expression of LL-37 gene and protein in A549 and MRC5 line cells. After preparing Valerian seeds, sowing them in March 2020, and harvesting the rhizome in October 2020, the extract was prepared fr...
Valeriana/efectos adversos,
Extractos Vegetales/efectos adversos,
Catelicidinas/efectos adversos,
Genes/genética,
Cromatografía Líquida de Alta Presión/métodos,
ddcs0244440,
Células A549/clasificación,
Hígado/anomalías,
Péptidos Catiónicos Antimicrobianos/agonistas,
d0024700,
Western Blotting/instrumentación
El ambiente obesogénico promueve la obesidad al facilitar el acceso y consumo de una amplia variedad de alimentos palatables altos en calorías. La activación del receptor de GLP1 (GLP1R) reduce la ingesta de alimentos, enlentece el vaciamiento gástrico y promueve un balance energético negativo a tra...
Dieta/efectos adversos,
Obesidad/metabolismo,
Obesidad/etiología,
Receptor del Péptido 1 Similar al Glucagón/metabolismo,
Exenatida/administración & dosificación,
Termogénesis,
Músculo Esquelético/metabolismo,
Irinotecán,
Proteína Desacopladora 2,
Ratones Endogámicos C57BL,
Ácidos Grasos/metabolismo,
Oxidación-Reducción,
Western Blotting,
Inyecciones Intraperitoneales
Abstract Lung cancer is a major cause of cancer-related death worldwide. This study investigated the regulatory effects of the microRNA-99a-5p (miR-99a-5)/VLDLR axis on lung cancer cell sensitivity to chemotherapy and its mechanism. miR-99a-5p and VLDLR expression levels were quantified using RT-qPCR and...
Abstract This study aimed to investigate the role and signaling pathways of β3-AR in myocardial ischemia/reperfusion (I/R) injury, which is one of the leading causes of death worldwide. 47 male rats were randomly divided into two main groups to evaluate infarct size and molecular parameters. Rats in...
SUMMARY: This study is to investigate the role and mechanism of RGD peptide in laryngeal cancer stem cells (CSCs). Laryngeal cancer CD133+Hep-2 CSCs were sorted by flow cytometry. RGD peptide was co-cultured with sorted laryngeal CSCs. Cell proliferation was detected with CCK-8 assay. The mRNA levels of ...
Oligopéptidos/metabolismo,
Integrina alfaVbeta3/antagonistas & inhibidores,
Neoplasias Laríngeas,
Factor A de Crecimiento Endotelial Vascular/antagonistas & inhibidores,
Proliferación Celular,
Western Blotting,
Citometría de Flujo,
Inmunohistoquímica,
Factor A de Crecimiento Endotelial Vascular/genética,
ARN Mensajero/antagonistas & inhibidores,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Células Madre Neoplásicas,
Neovascularización Patológica,
Cartilla de ADN
SUMMARY: This study is to investigate the regulation of Notch1 and Foxp1 by miR-34a in the development of psoriasis vulgaris. RT-PCR was used to compare the levels of miR-34a in the skin lesions of 20 patients with psoriasis vulgaris and 20 normal skin tissues. Immunohistochemistry was used to detect the...
Psoriasis/genética,
Psoriasis/metabolismo,
MicroARNs/genética,
MicroARNs/metabolismo,
Receptor Notch1/genética,
Receptor Notch1/metabolismo,
Factores de Transcripción Forkhead/genética,
Factores de Transcripción Forkhead/metabolismo,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Inmunohistoquímica,
Transfección,
Western Blotting
Abstract Doxorubicin (DOX) induced myocardial toxicity may limit its therapeutic use in clinic. Psoralen (PSO), a major active tricyclic furocoumarin extracted from Psoralea corylifolia, is widely used as an antineoplastic agent in treatment of leukemia and other cancers. This study is aim to find the pr...
A maioria das respostas alérgicas a alimentos é mediada por IgE, que pode ser detectada para fins de diagnóstico da alergia alimentar. No entanto, para isso é necessário que alérgenos purificados estejam disponíveis para a elaboração dos diferentes formatos de ensaio, inclusive por microarray, q...
Neutrophils are polymorphonuclear leukocytes that play a key role in the organism defense. These cells enroll in a range of actions to ensure pathogen elimination and orchestrate both innate and adaptative immune responses. The main physiological structures of neutrophils are their storage organelles tha...
