Resumen La espectrometría de masas MALDI-TOF MS es una técnica rápida y sencilla para identificar microorganismos por análisis proteico. Se estudiaron 304 aislados de levaduras procedentes de micosis superficiales y profundas, con el objetivo de comparar tres métodos: convencional (bioquímico y mor...
O objetivo deste estudo foi de avaliar a atividade antifúngica de uma solução à base de glucoprotamina (GLU) e de óleo essencial (OE) de Cymbopogon citratus. Candida tropicalis, Claviceps lusitaniae, Meyerozyma (Pichia) guilliermondii e Candida parapsilosis foram isoladas das su...
Background: Fermentation strategies for bioethanol production that use flocculating Saccharomyces cerevisiae yeast need to account for the mechanism by which inhibitory compounds, generated in the hydrolysis of lignocellulosic materials, are tolerated and detoxified by a yeast floc. Results: Diffusion co...
Objetivo: O Compêndio de Métodos e de Boas Práticas em Coleção de Cultura de Leveduras do Instituto de Biologia do Exército (IBEx) foi elaborado com o propósito de operacionalizar as atividades de pesquisa, garantindo o desempenho seguro e confiável dos seus objetivos regimentais. Métodos: A Col...
Davel, Graciela O;
Mazza, Mariana;
Refojo, Nicolás;
Taverna, Constanza G;
Rivas, María C;
Fernández, Julián;
Vivot, Matías E;
Mazza, Marta G;
Mastromónaco, Gladys M;
Bueno, Nadia S;
Molina, Viviana;
Brero, María L;
Kornblit, Fernando.
Reference fungal cultures (RFCs) are essential for the internal quality control of laboratories. The production of these cultures requires standardized procedures (IRAM 14950:2016 and ISO 17034:2016 standards) carried out by a recognized and accredited laboratory. The aim of this work was to produce RFC ...
The surface of grapes lodges a complex community of yeast species responsible for spontaneous alcoholic fermentation. The study of indigenous Saccharomyces and "non-Saccharomyces" yeasts during grape must fermentation constitutes a major research area in microbial enology. Although there are detailed stu...
Background: Yarrowia lipolytica is a nonconventional, dimorphic yeast with multiple biotechnological applications. Considering the size of Y. lipolytica cells and a plethora of its morphological forms (spherical cells or hyphae and pseudohyphae), it is highly difficult to select a suitable carrier for th...
Yarrowia/metabolismo,
Yarrowia/química,
Inmovilización,
Celulosa/metabolismo,
Aceites de Plantas,
Levaduras,
Emulsiones,
Nanoestructuras,
Polímeros,
Biotecnología,
Porosidad
Background: Sugars from sweet sorghum stalks can be used to produce ethanol and also to grow oleaginous yeasts. Instead of two separate processes, in this paper we propose a different route producing ethanol and microbial oil in two consecutive fermentation steps. Results: Three yeasts were compared in t...
Etanol/metabolismo,
Biocombustibles/microbiología,
Sorghum/química,
Fermentación,
Jugos de Frutas y Vegetales,
Zumos,
Levaduras,
Trichosporon,
Lipomyces,
Saccharomyces cerevisiae,
Azúcares
Background: In industrial yeasts, selection and breeding for resistance to multiple stresses is a focus of current research. The objective of this study was to investigate the tolerance to multiple stresses of Saccharomyces cerevisiae obtained through an adaptive laboratory evolution strategy involving a...
Saccharomyces cerevisiae/fisiología,
Saccharomyces cerevisiae/genética,
Adaptación Fisiológica,
Etanol/síntesis química,
Selección Genética,
Cruzamiento,
Fermentación,
Estrés Fisiológico,
Hipergravedad,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Proteínas de Choque Térmico,
Congelación,
Trehalosa,
Levaduras
Abstract Birds of prey and from Psittacidae family are host to fungal microbiota and play an important role in the epidemiology of zoonoses. Few studies in the literature have characterized mycelial and yeast fungi in the droppings of these birds and correlated the isolates with the zoonotic potential o...
