Results: 57

Development and characterization of polyclonal antibodies against the linker region of the telomere-binding protein TRF2

Background: TRF2 (telomeric repeat binding factor 2) is an essential component of the telomere-binding protein complex shelterin. TRF2 induces the formation of a special structure of telomeric DNA and counteracts activation of DNA damage-response pathways telomeres. TRF2 has a poorly characterized linker...

Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain

Braz. j. microbiol; 49 (1), 2018
ABSTRACT Bacillus anthracis strain SPV842_15 was isolated from bovine fetus, while B. anthracis strain Brazilian vaccinal was recovered from a commercial vaccine. We report here the genome sequences of both strains. The SPV842_15 genome is composed of a single circular chromosome with a length of 5,228,6...

Complete genome sequence of human pathogen Kosakonia cowanii type strain 888-76T

Braz. j. microbiol; 49 (1), 2018
ABSTRACT Kosakonia cowanii type strain 888-76T is a human pathogen which was originally isolated from blood as NIH group 42. In this study, we report the complete genome sequence of K. cowanii 888-76T. 888-76T has 1 chromosome and 2 plasmids with a total genome size of 4,857,567 bp and C+G 56.15%. This g...

Resistencia plasmídica a colistin por el gen mcr-1 en Enterobacteriaceae en Paraguay

La resistencia a las polimixinas mediada por plásmidos (gen mcr-1) representa una amenaza para la salud pública, puesto que colistina es utilizada en la práctica médica como una de las últimas alternativas para el tratamiento de gérmenes multiresistentes. Este estudio describe la circulaciónde cep...

Survey on pathogenic Escherichia coli and Salmonella spp. in captive cockatiels (Nymphicus hollandicus)

Braz. j. microbiol; 49 (supl.1), 2018
Abstract We surveyed healthy captive cockatiels (Nymphicus hollandicus) for Escherichia coli and Salmonella spp. Cloacal swabs were collected from 94 cockatiels kept in commercial breeders, private residencies and pet shops in the cities of São Paulo/SP and Niterói/RJ (Brazil). Three strains of E. coli...

Importance of the putative furin recognition site 742RNRR745 for antiangiogenic Sema3C activity in vitro

Braz. j. med. biol. res; 51 (11), 2018
Angiogenesis is one of the key processes in the growth and development of tumors. Class-3 semaphorins (Sema3) are characterized as axon guidance factors involved in tumor angiogenesis by interacting with the vascular endothelial growth factor signaling pathway. Sema3 proteins convey their regulatory sign...

Quorum sensing LuxS/autoinducer-2 inhibits Enterococcus faecalis biofilm formation ability

J. appl. oral sci; 26 (), 2018
Abstract Objective: To investigate the relation between biofilm formation ability and quorum sensing gene LuxS/AI-2. Materials and Methods: Enterococcus faecalis (E. faecalis) standard strain ATCC 29212 was used in the study. Long flanking homology polymerase chain reaction method was used to build the...

Caracterización de los plásmidos de cepas de escherichia coli aislados de coprocultivos de niños sanos
Characterization of plasmids of escherichia coli strains isolated from stool culture of healthy children

Rev. Inst. Nac. Hig; 49 (2), 2018
La microbiota intestinal representa una reserva potencial de organismos resistentes a los antimicrobianos, y el sitio donde los genes de resistencia pueden ser transferidos desde la microbiota comensal a los microorganismos virulentos. En este trabajo se caracterizaron los perfiles fenotípicos de resist...

Complete genome sequence of a phthalic acid esters degrading Mycobacterium sp. YC-RL4

Braz. j. microbiol; 48 (4), 2017
ABSTRACT Mycobacterium sp. YC-RL4 is capable of utilizing a broad range of phthalic acid esters (PAEs) as sole source of carbon and energy for growth. The preliminary studies demonstrated its high degrading efficiency and good performance during the bioprocess with environmental samples. Here, we present...

RecET recombination system driving chromosomal target gene replacement in Zymomonas mobilis

Background: Zymomonas mobilis is a Gram-negative microaerophilic bacterium with excellent ethanol-producing capabilities. The RecET recombination system provides an efficient tool for direct targeting of genes in the bacterial chromosome by PCR fragments. Results: The plasmids pSUZM2a-RecET and pSUZM2a-R...