Results: 723

Modulation of the prostaglandin-endoperoxide synthase 2 gene expression by variant haplotypes: influence of the 3'-untranslated region

Braz. j. med. biol. res; 51 (2), 2018
The inducible inflammatory enzyme cycloxigenase-2 is up-regulated in cancer, and favors tumor progression. Cycloxigenase-2 is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, which presents sequence variations in the promoter region (PR) and in the 3′-untranslated region (3′...

Multiplex polymerase chain reaction as an improved method for screening Histoplasma capsulatum mating types

Mem. Inst. Oswaldo Cruz; 113 (10), 2018
Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). Th...

Imported case of Dengue virus 3 genotype I in Rio de Janeiro state, Brazil

Mem. Inst. Oswaldo Cruz; 113 (8), 2018
The dengue virus (DENV), of the genus Flavivirus (Flaviviridae), has four antigenically distinct serotypes, of which DENV-3 is classified into five genotypes. Here, we describe the detection of DENV-3 genotype I in sera of a Brazilian patient travelling from Singapore to Rio de Janeiro, Brazil, by using ...

Potential overestimation of HIV-1 sub-subtype F1 circulation in Rio de Janeiro, Brazil

Mem. Inst. Oswaldo Cruz; 113 (8), 2018
In Brazil, detection of the HIV-1 sub-subtype F1 has decreased with a simultaneous increase in detection of the recombinant FB and FC forms. In previous HIV-1 env molecular epidemiology studies in Rio de Janeiro, 11.4% of the detected sequences were of the F1 sub-subtype. With the goal of re-estimating t...

Fatal toxoplasmosis in a southern muriqui (Brachyteles arachnoides) from São Paulo state, Brazil: Pathological, immunohistochemical, and molecular characterization

We report the pathological, immunohistochemical, and molecular features of fatal acute systemic toxoplasmosis in an adult, female, free‐living southern muriqui (Brachyteles arachnoides) from São Paulo state, Brazil. PCR‐RFLP genotyping analysis identified the #21 genotype of Toxoplasma gondii. This ...

Análise da associação entre a periodontite e polimorfismos de nucleotídeo único da interleucina-1beta

Periodontia; 28 (2), 2018
A periodontite é uma doença inflamatória, que pode ser classificada em Periodontite Crônica (PC) ou Periodontite Agressiva (PA), desencadeada por um desequilíbrio na microbiota subgengival, que pode ser influenciado por diversos fatores, como polimorfismos genéticos. Dessa forma, o objetivo deste t...

Advances in Cryptococcus genomics: insights into the evolution of pathogenesis

Mem. Inst. Oswaldo Cruz; 113 (7), 2018
Cryptococcus species are the causative agents of cryptococcal meningitis, a significant source of mortality in immunocompromised individuals. Initial work on the molecular epidemiology of this fungal pathogen utilized genotyping approaches to describe the genetic diversity and biogeography of two species...

Description of a novel variant in the MAMLD1 gene in isolated hypospadias

Urol. colomb; 27 (1), 2018
Introduction Hypospadias is a multifactorial malformation. Among previously described genes, the MAMLD1 has recently been identified in association with the development of hypospadias. So far, there are no studies in Latin America addressing this gene. The aim of this paper is to describe the presence ...

Caracterización molecular y patrón de susceptibilidad antimicrobiana de Escherichia coli productora de β -lactamasas de espectro extendido en infección del tracto urinario adquirida en la comunidad

Rev. chil. infectol; 35 (1), 2018
Resumen Introducción Las infecciones del tracto urinario adquiridas en la comunidad (ITUac) causadas por cepas de Escherichia coli productoras de β-lactamasas de espectro extendido (BLEE), principalmente por cepas que contienen el gen blaCTX-M-15, es un fenómeno creciente a nivel mundial. Objeti...

Polimorfismos genéticos asociados a la inmunidad innata y la susceptibilidad genética a la tuberculosis

Resumen La tuberculosis es un problema de salud mundial exacerbado por la ausencia de una vacuna eficaz y la emergencia de cepas multidrogo resistentes. La inmunidad innata, clave en la susceptibilidad a tuberculosis, está asociada a polimorfismos genéticos en TLRs (Toll Like Receptors), VDR (Vitamin D...