Results: 496

Lentivirus mediated silencing of Ubiquitin Specific Peptidase 39 inhibits cell proliferation of human hepatocellular carcinoma cells in vitro

Biol. Res; 48 (), 2015
BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcino...

Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress

Electron. j. biotechnol; 17 (6), 2014
Background Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to analysis...

Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts

Electron. j. biotechnol; 17 (5), 2014
Background Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector were...

Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)

Electron. j. biotechnol; 17 (5), 2014
Background Head smut of maize, which is caused by Sporisorium reilianum f. sp. zeae (Kühn), is a serious disease in maize. In order to reveal the molecular mechanism of the resistance to head smut in maize, a microarray containing ~ 14,850 probes was used to monitor the gene expression profiles between ...

Expression profiles and polymorphism analysis of CDIPT gene on Qinchuan cattle

Electron. j. biotechnol; 17 (4), 2014
Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recen...

Lesiones preneoplásicas gástricas en pacientes colombianos: asociación de polimorfismos genéticos interleucinas 1B-511, 1RN, 10-819, 10-1082, factor de necrosis tumoral-&alpha -308 y anticuerpos inmunoglobulina G hacia cagA de Helicobacter pylori

Rev. colomb. cancerol; 18 (1), 2014
Objetivo: Evaluar la asociación de los polimorfismos de alguna de las citocinas más estudiadas en relación con el cáncer gástrico (IL-1B-511, IL-1RN intron-2-VNTR, TNF-α-308, IL-10-819 e IL-10- 1082) y la presencia de anticuerpos hacia la proteína cagA de Helicobacter pylori con las lesiones ...

Avaliação de marcadores de prognóstico no câncer de mama e análise funcional de CIP4

O câncer de mama é uma doença extremamente heterogênea compreendendo diferentes subtipos moleculares que resultam em evoluções clínicas e condutas terapêuticas distintas. A maior gravidade desta patologia está associada a sua capacidade de formação de metástases Mudanças no padrão de expres...

A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting

Biol. Res; 47 (), 2014
BACKGROUND: Human Immunodeficiency Virus Type 1 (HIV-1) viral load testing at regular intervals is an integral component of disease management in Acquired Immunodeficiency Syndrome (AIDS) patients. The need in countries like India is therefore an assay that is not only economical but efficient and highly...

Growth differentiation factor 9 and bone morphogenetic protein 15 expression in previtellogenic oocytes and during early embryonic development of Yellow-tail Kingfish Seriola lalandi

Biol. Res; 47 (), 2014
BACKGROUND: During fish oocyte maturation, specific molecules are expressed and accumulated within oocyte until fertilization and embryo development. Special attention have been paid in members of the transforming growth factor (TGF-β) superfamily; growth differentiation factor 9 (GDF9/gdf9) and bone mo...

Expression of TRF2 and its prognostic relevance in advanced stage cervical cancer patients

Biol. Res; 47 (), 2014
BACKGROUND: Telomeres are protective caps consisted of specific tandem repeats (5'-TTAGGG-3'). Shortening of telomeres at each cell division is known as "mitotic clock" of the cells, which renders telomeres as important regulators of lifespan. TRF2 is one of the critical members of shelterin complex, whi...