Abstract Anaplasma marginale and piroplasm species are widespread among Brazilian cattle herds. Both of these tick-borne pathogens hamper livestock production and cause a significant economic impact. Although buffaloes have demonstrated a high level of adaptability, data on tick-borne pathogens are scarc...
Silva, Marcio André;
Pena, Hilda Fátima Jesus;
Soares, Herbert Sousa;
Aizawa, Juliana;
Oliveira, Solange;
Alves, Bruna Farias;
Souza, Dênisson Silva;
Melo, Renata Pimentel Bandeira;
Gennari, Solange Maria;
Mota, Rinaldo Aparecido;
Silva, Jean Carlos Ramos.
Abstract Recent genetic population studies on Toxoplasma gondii in Brazil have shown large genetic variability. The objective of the present study was to isolate and genotypically characterize T. gondii from free-ranging and captive wild mammals and birds in Pernambuco state, Brazil. Fragments of heart,...
Anticuerpos Antiprotozoarios/sangre,
Aves/parasitología,
Brasil,
ADN Protozoario/análisis,
Variación Genética,
Genotipo,
Mamíferos/clasificación,
Mamíferos/parasitología,
Reacción en Cadena de la Polimerasa,
Polimorfismo de Longitud del Fragmento de Restricción,
Toxoplasma/genética,
Toxoplasma/inmunología,
Toxoplasma/aislamiento & purificación
Abstract Toxoplasma gondii presents a high prevalence worldwide, infecting several animals. Felines are considered the definitive hosts and among the intermediate hosts we highlight mammals and birds. The man can become infected by ingesting tissue cysts present in birds and mammals. Biological and molec...
Pruebas de Aglutinación,
Anticuerpos Antiprotozoarios/sangre,
Brasil,
Pollos/parasitología,
ADN Protozoario/análisis,
Genotipo,
Ratones Endogámicos BALB C,
Reacción en Cadena de la Polimerasa,
Polimorfismo de Longitud del Fragmento de Restricción,
Enfermedades de las Aves de Corral/diagnóstico,
Enfermedades de las Aves de Corral/parasitología,
Toxoplasma/genética,
Toxoplasma/aislamiento & purificación,
Toxoplasma/patogenicidad,
Toxoplasmosis Animal/diagnóstico
Ferreira, Fernanda Pinto;
Caldart, Eloiza Teles;
Freire, Roberta Lemos;
Mitsuka-Breganó, Regina;
Freitas, Felipe Machado de;
Miura, Ana Carolina;
Mareze, Marcelle;
Martins, Felippe Danyel Cardoso;
Urbano, Mariana Ragassi;
Seifert, Adilson Luiz;
Navarro, Italmar Teodorico.
Abstract The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Tox...
Cryptosporidium/genética,
Cryptosporidium/aislamiento & purificación,
ADN Protozoario/genética,
Giardia lamblia/genética,
Giardia lamblia/aislamiento & purificación,
Reacción en Cadena de la Polimerasa,
Suelo/parasitología,
Toxoplasma/genética,
Toxoplasma/aislamiento & purificación,
Verduras/parasitología,
Agua/parasitología
Abstract The aim of this study was to identify Plasmodium spp. in blood samples from nonhuman primates (NHPs) in the state of Maranhão, using classical and alternative techniques for examination of human malaria. A total of 161 blood samples from NHPs were analyzed: 141 from captive animals at a Wildli...
Anticuerpos Antiprotozoarios/sangre,
ADN Protozoario/sangre,
Técnica del Anticuerpo Fluorescente Indirecta,
Malaria/diagnóstico,
Malaria/parasitología,
Malaria/veterinaria,
Plasmodium/genética,
Plasmodium/inmunología,
Platirrinos/parasitología,
ARN Ribosómico 18S/sangre,
Reacción en Cadena en Tiempo Real de la Polimerasa
Zahirnia, Amir Hossein;
Bordbar, Ali;
Ebrahimi, Sahar;
Spotin, Adel;
Mohammadi, Somayeh;
Ghafari, Seyedeh Maryam;
Ahmadvand, Setareh;
Jabbari, Negar;
Esmaeili Rastaghi, Ahmad Reza;
Parvizi, Parviz.
ABSTRACT Background Leishmania major is a causative agent of zoonotic cutaneous leishmaniasis in the center of Iran, Abarkouh district. Molecular characterization and precise incrimination of Leishmania species was carried out to perform controlling measurements and to design treatment programs for zoon...
ADN Protozoario/genética,
ADN Protozoario/aislamiento & purificación,
Enfermedades Endémicas,
Haplotipos,
Irán,
Leishmania major/genética,
Leishmania major/aislamiento & purificación,
Leishmania tropica/genética,
Leishmania tropica/aislamiento & purificación,
Leishmania tropica/ultraestructura,
Leishmaniasis Cutánea/epidemiología,
Leishmaniasis Cutánea/parasitología,
Leishmaniasis Cutánea/patología,
Reacción en Cadena de la Polimerasa,
Polimorfismo de Longitud del Fragmento de Restricción,
Población Rural
Abstract INTRODUCTION: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests...
Anticuerpos Monoclonales/sangre,
Anticuerpos Antiprotozoarios/sangre,
Formación de Anticuerpos,
Biopsia,
Estudios de Casos y Controles,
Enfermedad de Chagas/diagnóstico,
ADN Protozoario/análisis,
Endocardio/parasitología,
Inmunohistoquímica,
Reacción en Cadena de la Polimerasa,
Sensibilidad y Especificidad,
Trypanosoma cruzi/inmunología
Abstract INTRODUCTION This study proposes to identify the Leishmania species found in the skin lesions of cutaneous leishmaniasis (CL) patients from Brasiléia municipality (Acre). METHODS Skin biopsy imprints or biopsy fragments were assayed via kDNA-PCR/RFLP and FRET-real-time PCR. RESULTS Of ind...
Biopsia,
Brasil/epidemiología,
ADN de Cinetoplasto/genética,
ADN Protozoario/genética,
Enfermedades Endémicas,
Leishmania braziliensis/genética,
Leishmania guyanensis/genética,
Leishmaniasis Cutánea/diagnóstico,
Leishmaniasis Cutánea/epidemiología,
Polimorfismo de Longitud del Fragmento de Restricción,
Reacción en Cadena en Tiempo Real de la Polimerasa
Vieira, Osires Lustosa Eloi;
Macedo, Lucia Oliveira de;
Santos, Marcos Antônio Bezerra;
Silva, José Augusto Bastos Afonso;
Mendonça, Carla Lopes de;
Faustino, Maria Aparecida da Gloria;
Ramos, Carlos Alberto do Nascimento;
Alves, Leucio Câmara;
Ramos, Rafael Antonio Nascimento;
Carvalho, Gílcia Aparecida de.
Abstract Trypanosoma (Duttonella) vivax is an important cause of economic losses among feedlot cattle. These losses are related to the morbidity, mortality, reproductive issues and decreased production. It is known that the clinical signs observed in infections by this protozoon are similar to other hemo...
Camilo, Lilian Muniz;
Pereira-Chioccola, Vera Lucia;
Gava, Ricardo;
Meira-Strejevitch, Cristina da Silva;
Vidal, Jose Ernesto;
Mattos, Cinara Cássia Brandão de;
Frederico, Fábio Batista;
De Mattos, Luiz Carlos;
Spegiorin, Lígia Cosentino Junqueira Franco;
Murata, Fernando Henrique Antunes;
Ferreira, Marina Neves;
Barbosa, Deusenia Machado Ulisses;
Gonçalves, Fausto da Silva;
Dias, Cristiane Moraes;
Catelan, Marcia Wakai;
Siqueira, Rubens Camargo;
Previato, Mariana;
Barbosa, Amanda Pires;
Cavallini, Danilo.
ABSTRACT Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR testing two primer sets (B...
