Results: 97

La memoria del agua, una historia que ya no puede soslayarse
The memory of water, a story that can no longer be ignored

Homeopatia Méx; 84 (696), 2015
Toda esta historia inició hace millones de años, pero fue hasta que Samuel Hahnemann desarrolló el método homeopático y propuso dinamizar los medicamentos homeopáticos (que no es otra cosa que triturar y mezclar con lactosa y diluir y agitar en alcohol/agua) que se empezó a estudiar el tema. Recie...
ADN, ARN

INXS, um longo RNA não codificador de proteínas mediador da apoptose

O splicing alternativo do pré-mRNA de BCL-X produz duas isoformas de mRNAs com funções antagônicas, a pró-apoptótica BCL-XS e a anti-apoptótica BCL-XL, cujo balanço regula a homeostasia celular. Entretanto, o mecanismo que regula esse processamento ainda é desconhecido. Nesse trabalho, nós iden...

Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation

Electron. j. biotechnol; 17 (6), 2014
Background Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and inves...

Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress

Electron. j. biotechnol; 17 (6), 2014
Background Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to analysis...

A novel chloroplastic isopentenyl diphosphate isomerase gene from Jatropha curcas: Cloning, characterization and subcellular localization

Electron. j. biotechnol; 17 (6), 2014
Background Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic and molluscicidal. But not much is known about the pat...

Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization

Electron. j. biotechnol; 17 (6), 2014
Background Peanut (Arachis hypogaea L.) is an important economic and oilseed crop. Long-term rainless conditions and seasonal droughts can limit peanut yields and were conducive to preharvest aflatoxin contamination. To elucidate the molecular mechanisms by which peanut responds and adapts to water limit...

Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)

Electron. j. biotechnol; 17 (5), 2014
Background Head smut of maize, which is caused by Sporisorium reilianum f. sp. zeae (Kühn), is a serious disease in maize. In order to reveal the molecular mechanism of the resistance to head smut in maize, a microarray containing ~ 14,850 probes was used to monitor the gene expression profiles between ...

Comparison of different methods for total RNA extraction from sclerotia of Rhizoctonia solani

Electron. j. biotechnol; 17 (1), 2014
Background Rhizoctonia solani (teleomorph: Thanatephorus cucumeris) is one of the most important pathogens of rice (Oryza sativa L.) that causes severe yield losses in all rice-growing regions. Sclerotia, formed from the aggregation of hyphae, are important structures in the life cycles of R. solani and ...

Ethylene treatment improves diosgenin accumulation in in vitro cultures of Dioscorea zingiberensis via up-regulation of CAS and HMGR gene expression

Electron. j. biotechnol; 16 (5), 2013
Background: The perennial medicinal herb Dioscorea zingiberensis is a very important plant used for steroid drug manufacturing for its high level of diosgenin in rhizome. Although the stimulation of diosgenin accumulation by ethylene has been reported in a few of plant species, its regulation is not yet ...