BACKGROUND: The domestic cat serves as an animal model for assisted reproductive studies of endangered felid species. To date, there are no data on the protein alterations following cryopreservation of oocytes in felid family. METHODS: Immature (germinal vesicle) domestic cat oocytes were vitrified in th...
Surfactants are amphipathic compounds containing both hydrophilic and hydrophobic groups, capable to lower the surface or interfacial tension. Considering the advantages of the use of biosurfactants produced by microorganisms, the aim of this paper was to develop and characterize a biosurfactant produced...
Análisis de Varianza,
Recuento de Colonia Microbiana,
Medios de Cultivo,
Electroforesis en Gel de Poliacrilamida,
Fermentación,
Concentración de Iones de Hidrógeno,
Líquenes/microbiología,
Valores de Referencia,
Semillas/efectos de los fármacos,
Aceite de Soja/química,
Espectroscopía Infrarroja por Transformada de Fourier,
Streptomyces/metabolismo,
Tensoactivos/metabolismo,
Factores de Tiempo,
Temperatura
Background: Escherichia coli has been widely used as a host to clone and express heterologous genes. However, there are few vectors available for cloning and expressing extremely toxic genes, which limits further basic and applied research on extremely toxic proteins. Results: In this study, a novel vect...
Escherichia coli/genética,
Proteínas de Escherichia coli/toxicidad,
Vectores Genéticos,
Triptófano/metabolismo,
Regiones Promotoras Genéticas,
Proteínas Co-Represoras,
Genes Bacterianos,
ARN sin Sentido,
Isopropil Tiogalactósido/metabolismo,
Reacción en Cadena de la Polimerasa,
Electroforesis en Gel de Poliacrilamida,
Desoxirribonucleasa BamHI/metabolismo,
Western Blotting,
Clonación Molecular
Abstract Background: Angiotensin II (Ang II), the primary effector hormone of the renin-angiotensin system (RAS), acts systemically or locally, being produced by the action of angiotensin-converting-enzyme (ACE) on angiotensin I. Although several tissue RASs, such as cardiac RAS, have been described, li...
Background: Defense-related anti-oxidative response is a vital defense mechanism of plants against pathogen invasion. Ralstonia solanacearum is an important phytopathogen. Bacterial wilt caused by R. solanacearum is the most destructive disease and causes severe losses in patchouli, an important aromatic...
Pogostemon/enzimología,
Pogostemon/microbiología,
Pogostemon/inmunología,
Superóxido Dismutasa/metabolismo,
Catecol Oxidasa/metabolismo,
Ralstonia solanacearum/patogenicidad,
Virulencia,
Peroxidasa/metabolismo,
Fenilanina Amoníaco-Liasa/metabolismo,
Enzimas/metabolismo,
Electroforesis en Gel de Poliacrilamida Nativa,
Enzimas/inmunología,
Ralstonia solanacearum/fisiología,
Antioxidantes,
Electroforesis en Gel de Poliacrilamida
Background: Jatropha curcas is a wide-spreading latex-rich biodiesel plant with high oil content in seeds that have always been under intense studies. However, studies are lacking on the latex component that is considered rich in proteins with potentially important physiological functions and secondary m...
Jatropha,
Tallos de la Planta/química,
Látex/química,
Proteínas de Plantas/análisis,
Proteínas de Plantas/aislamiento & purificación,
Proteómica/métodos,
Fenol/química,
Acetona/química,
Cromatografía Liquida,
Espectrometría de Masas en Tándem,
Espectrometría de Masa por Ionización de Electrospray,
Electroforesis en Gel de Poliacrilamida
A fim de se agregar valor ao resíduo farelo de trigo gerado em indústrias do setor alimentício avaliou-se, no presente trabalho, o potencial deste subproduto como substrato para produção de enzima xilanase no cultivo em estado sólido, utilizando consórcios fúngicos bem como os fungos Aspergill...
Serum protein concentrations, including acute phase proteins (APPs), of goats and ewes with naturally acquired Sthaphylococcus aureus mastitis were determined by means of SDS-PAGE electrophoresis to evaluate the relevance of these APPs as biomarkers of the disease in these species. Fifteen healthy goats ...
Antiinfecciosos/química,
Cabras/virología,
Ovinos/virología,
Mastitis/veterinaria,
Proteínas de Fase Aguda/análisis,
Staphylococcus aureus,
Ceruloplasmina/análisis,
Fibrinógeno/análisis,
Haptoglobinas/análisis,
Orosomucoide/análisis,
Electroforesis en Gel de Poliacrilamida/veterinaria
Protein electrophoresis is a relatively simple technique that allows separating serum protein fractions, and provides important information in the investigation and diagnosis of several diseases. This study determined the levels of acute-phase proteins in the serum of healthy, captive emus (Dromaius nova...
ABSTRACT Purpose: Avastin® (bevacizumab) is an anti-vascular endothelial growth factor (VEGF) monoclonal antibody given as an off-label drug by intravitreal administration for treatment of ocular diseases. The drug's clinical application and its cost-benefit profile has generated demand for its divisio...
Inhibidores de la Angiogénesis/análisis,
Inhibidores de la Angiogénesis/química,
Bevacizumab/análisis,
Bevacizumab/química,
Cromatografía en Gel/métodos,
Embalaje de Medicamentos,
Estabilidad de Medicamentos,
Dispersión Dinámica de Luz/métodos,
Electroforesis en Gel de Poliacrilamida/métodos,
Ensayo de Inmunoadsorción Enzimática/métodos,
Inyecciones Intravítreas,
Peso Molecular,
Nefelometría y Turbidimetría/métodos,
Control de Calidad,
Factor A de Crecimiento Endotelial Vascular/análisis
