Abstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood w...
Anemia/diagnóstico,
Anemia/microbiología,
Anemia/parasitología,
Anemia/veterinaria,
ADN Protozoario/genética,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/microbiología,
Enfermedades de los Perros/parasitología,
Ehrlichia canis/genética,
Ehrlichiosis/diagnóstico,
Ehrlichiosis/veterinaria,
Eucoccidiida/genética,
Filogenia,
Piroplasmida/genética,
Infecciones Protozoarias en Animales/diagnóstico,
Infecciones Protozoarias en Animales/microbiología,
Infecciones Protozoarias en Animales/parasitología,
ARN Ribosómico 18S,
Trombocitopenia/diagnóstico,
Trombocitopenia/microbiología,
Trombocitopenia/parasitología,
Trombocitopenia/veterinaria
Abstract INTRODUCTION Peripheral blood of 400 dogs infected with Leishmania and Ehrlichia were analyzed using polymerase chain reaction (PCR), and clinical signs were characterized. METHODS PCR and parasitological tests were conducted. RESULTS PCR was positive for Leishmania in 84.75%, and parasito...
Brasil/epidemiología,
Coinfección,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Ehrlichiosis/diagnóstico,
Ehrlichiosis/epidemiología,
Ehrlichiosis/veterinaria,
Enfermedades Endémicas,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Reacción en Cadena de la Polimerasa,
Sensibilidad y Especificidad
Abstract INTRODUCTION: The aim of this study was to determine the seroprevalence and risk factors associated with canine visceral leishmaniasis in Paraíba State. METHODS: Blood samples were collected from 411 dogs in four municipalities of Paraíba State. The seroprevalence was assessed by using ELISA...
Abstract INTRODUCTION: Herein, we assessed the seroprevalence and spatial distribution of Leishmania infantum in dogs in Garanhuns, Northeastern Brazil. METHODS: Sera samples (n = 242) were analyzed using an enzyme-linked immunosorbent assay (ELISA). The spatial distribution of dogs seropositive for ...
Anticuerpos Antiprotozoarios/sangre,
Brasil/epidemiología,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Enfermedades Endémicas/veterinaria,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Leishmania infantum/inmunología,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Estudios Seroepidemiológicos,
Análisis Espacial
Abstract This report describes the first autochthonous case of canine visceral leishmaniasis in Rondônia, northern Brazil. A canine resident of the municipality of Cacoal, with clinical signs and symptoms of visceral leishmaniasis, was treated by a veterinarian. Samples were analyzed by a reference labo...
Anticuerpos Antiprotozoarios/sangre,
Brasil/epidemiología,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Ensayo de Inmunoadsorción Enzimática/veterinaria,
Técnica del Anticuerpo Fluorescente Indirecta/veterinaria,
Leishmania infantum/genética,
Leishmania infantum/inmunología,
Leishmaniasis Visceral/diagnóstico,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Reacción en Cadena de la Polimerasa/veterinaria
Abstract A modified TaqMan real-time polymerase chain reaction targeting a 138 bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently heal...
Proteínas de la Membrana Bacteriana Externa/genética,
Proteínas de la Membrana Bacteriana Externa/orina,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/orina,
Leptospira/genética,
Leptospira/aislamiento & purificación,
Leptospirosis/diagnóstico,
Leptospirosis/microbiología,
Leptospirosis/orina,
Leptospirosis/veterinaria,
Lipoproteínas/genética,
Lipoproteínas/orina,
Reacción en Cadena en Tiempo Real de la Polimerasa/métodos,
Sensibilidad y Especificidad,
Orina/microbiología
Dias, Renata Cristina Ferreira;
Thomaz-Soccol, Vanete;
Pasquali, Aline Kuhn Sbruzzi;
Alban, Silvana Maria;
Fendrich, Ricardo Cancio;
Pozzolo, Eliane Maria;
Chiyo, Luciana;
Bisetto Júnior, Alceu;
Ferreira, Fernanda Pinto;
Desto, Stela Silva;
Freire, Roberta Lemos;
Mitsuka-Breganó, Regina;
Navarro, Italmar Teodorico.
Abstract The aim of this study was to investigate the occurrence of anti-Leishmania spp. antibodies in dogs from localities in the city of Foz do Iguaçu, Paraná state, Brazil, on the border with Argentina and Paraguay. Blood samples dogs were collected to perform the following serologic tests: immunoc...
Anticuerpos Antiprotozoarios/sangre,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Ensayo de Inmunoadsorción Enzimática,
Técnica del Anticuerpo Fluorescente Indirecta,
Leishmania/inmunología,
Leishmaniasis Visceral,
Leishmaniasis Visceral/epidemiología,
Leishmaniasis Visceral/veterinaria,
Prevalencia,
Brasil
Abstract INTRODUCTION: This study aimed to detect anti-Leptospira spp antibodies and Leptospira DNA in domestic dogs. METHODS: Blood and urine from 106 dogs were evaluated by microscopic agglutination test (MAT) and polymerase chain reaction (PCR), respectively. RESULTS: Six (5.7%) and one (1%) ani...
Pruebas de Aglutinación/veterinaria,
Brasil/epidemiología,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Enfermedades de los Perros/microbiología,
Leptospira/clasificación,
Leptospira/genética,
Leptospira/inmunología,
Leptospirosis/diagnóstico,
Leptospirosis/epidemiología,
Leptospirosis/microbiología,
Leptospirosis/veterinaria,
Reacción en Cadena de la Polimerasa/veterinaria,
Prevalencia
Freitas, Yannara Barbosa Nogueira;
Souza, Celeste da Silva Freitas de;
Magalhães, Jamille Maia e;
Sousa, Maressa Laíse Reginaldo de;
d'Escoffier, Luiz Ney;
Valle, Tânia Zaverucha do;
Gonçalves, Teresa Cristina Monte;
Gil-Santana, Hélcio Reinaldo;
Kazimoto, Thais Aaparecida;
Amora, Sthenia Santos Albano.
Abstract INTRODUCTION: Chagas disease is caused by the protozoa Trypanosoma cruzi. Its main reservoir is the domestic dog, especially in rural areas with favorable characteristics for vector establishment and proliferation. The aims of this study were to collect data, survey and map the fauna, and iden...
Anticuerpos Antiprotozoarios/sangre,
Brasil/epidemiología,
Enfermedad de Chagas/diagnóstico,
Enfermedad de Chagas/epidemiología,
Enfermedad de Chagas/veterinaria,
Enfermedades de los Perros/diagnóstico,
Enfermedades de los Perros/epidemiología,
Técnica del Anticuerpo Fluorescente Indirecta,
Insectos Vectores/parasitología,
Reacción en Cadena de la Polimerasa,
Prevalencia,
Población Rural,
Triatominae/parasitología,
Trypanosoma cruzi/inmunología
Nogueira, Camila Tita;
Cistia, Mayara Lúcia Del;
Urbaczek, Ana Carolina;
Jusi, Márcia MG;
Velásquez, Angela Maria Arenas;
Machado, Rosângela Zacarias;
Ferreira, Henrique;
Henrique-Silva, Flávio;
Langoni, Hélio;
Costa, Paulo Inácio da;
Graminha, Márcia AS.
Visceral leishmaniasis (VL) is fatal if left untreated. Infected dogs are important reservoirs of the disease, and thus specific identification of infected animals is very important. Several diagnostic tests have been developed for canine VL (CVL); however, these tests show varied specificity and sensiti...
