Results: 47

Characterization of the variable region in the class 1 integron of antimicrobial-resistant Escherichia coli isolated from surface water

Canal, Natália; Meneghetti, Karine Lena; Almeida, Clara Ponzi de; Bastos, Marina da Rosa; Otton, Letícia Muner; Corção, Gertrudes.

Braz. j. microbiol; 47 (2), 2016

Abstract Fecal bacteria are considered to be a potential reservoir of antimicrobial resistance genes in the aquatic environment and could horizontally transfer these genes to autochthonous bacteria when carried on transferable and/or mobile genetic elements. Such circulation of resistance genes constitut...

Antibacterianos/farmacología, Escherichia coli/genética, Escherichia coli/aislamiento & purificación, Agua Dulce/microbiología, Integrones, Brasil, Escherichia coli/efectos de los fármacos, Escherichia coli/metabolismo, Proteínas de Escherichia coli/genética, Proteínas de Escherichia coli/metabolismo, Variación Genética, Filogenia

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Loop-mediated isothermal amplification assays for screening of bacterial integrons

Yu, Guangchao; Chen, Lei; Lin, Chii-wann; Li, Bing; Cui, Hemiao; Chen, Siyi; Miao, Jian; Bian, Huawei; Chen, Dingqiang; Deng, Yang.

Biol. Res; 47 (), 2014

BACKGROUND: The occurrence and prevalence of integrons in clinical microorganisms and their role played in antimicrobial resistance have been well studied recently. As screening and detection of integrons are concerned, current diagnostic methodologies are restricted by significant drawbacks and novel me...

ADN Bacteriano/aislamiento & purificación, Integrones, Técnicas de Amplificación de Ácido Nucleico/métodos, Farmacorresistencia Bacteriana/genética, Escherichia coli/genética, Integrasas/genética, Reacción en Cadena de la Polimerasa/métodos, Salmonella/genética, Serratia marcescens/genética, Staphylococcus/genética, Vibrio cholerae/genética, Recuento de Colonia Microbiana, Cartilla de ADN, ADN Complementario, Electroforesis en Gel de Agar, Colorantes Fluorescentes, Calor, Pruebas de Sensibilidad Microbiana, Compuestos Orgánicos, Sensibilidad y Especificidad

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Clonagem, expressão e caracterização da nucleoproteína recombinante do vírus da bronquite infecciosa em Escherichia coli e em Pichia pastoris

Gibertoni, A. M; Gonçalves, M. C. M; Montassier, M. F. S; Fernandes, C. C; Montassier, H. J.

Arq. Inst. Biol. (Online); 77 (1), 2010

O gene da proteína de nucleocapsídeo (1.230 pb) da estirpe M41 do vírus da bronquite infecciosa (VBI) foi amplificado pelas reações de transcrição reversa e em cadeia da polimerase (RT-PCR) e clonado, em seguida, em dois sistemas; pET28a - Escherichia coli e pFLD -Pichia pastoris. Os produtos reco...

Proteínas de la Nucleocápside/ultraestructura, Virus de la Bronquitis Infecciosa/ultraestructura, Escherichia coli/genética, Pichia/genética, Clonación Molecular, Ensayo de Inmunoadsorción Enzimática

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Clonal study of avian Escherichia coli strains by fliC conserved-DNA-sequence regions analysis

Campos, Tatiana Amabile de; Nakazato, Gerson; Stehling, Eliana Guedes; Brocchi, Marcelo; Silveira, Wanderley Dias da.

Pesqui. vet. bras; 28 (10), 2008

The clonal relationship among avian Escherichia coli strains and their genetic proximity with human pathogenic E. coli, Salmonela enterica, Yersinia enterocolitica and Proteus mirabilis, was determined by the DNA sequencing of the conserved 5' and 3'regions fliC gene (flagellin encoded gene). Among 30 co...

Escherichia coli/genética, Análisis de Secuencia de ADN, Células Clonales, Salmonella enterica/genética, Yersinia enterocolitica/genética, Proteus mirabilis/genética, Flagelina/análisis

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Construction of a cassette for cloning and analysis of replicons

Alonso, Guilhermina; Campos, Jenny; Bruzual, Igor; Rodríguez Lemoine, Vidal.

Acta cient. venez; 51 (1), 2000

The aim of this work was the construction of a cassette, i.e., a non-replicative molecule formed by linkage of an antibiotic resistance gene and a multiple cloning site. This cassette would allow the cloning and analysis of a wide range of replicons. The aac(6')-lc amikacin gene was isolated and ligated ...

Clonación Molecular, Replicón/genética, Plásmidos/genética, Farmacorresistencia Microbiana/genética, Plásmidos/efectos de los fármacos, Plásmidos/aislamiento & purificación, Amicacina/farmacología, Antibacterianos/farmacología, Ampicilina/farmacología, Penicilinas/farmacología, Escherichia coli/genética

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El fago lambda como vehículo de expresión molecular

Expression molecular with vehicle of DNA lambda

Padilla Bernal, Maria Patricia; Herrera Esparza, Rafael.

Rev. mex. reumatol; 9 (2), 1994

Bacteriófago lambda/genética, Escherichia coli/citología, Escherichia coli/genética, Lisogenia/genética, Lisogenia/inmunología, ADN/biosíntesis, ADN/genética, ADN/ultraestructura, Biología Molecular, Fagocitos/citología, Fagocitos/inmunología

Codificación cromosomal de fimbrias de Escherichia coli 36692 pielonefritogénica

Chromosomal coding of fimbriae of pyeloneprhitogenic escherichia coli 36692

Padilla, C; Zemelman, R; Mondaca, M; Vivaldi, E.

Rev. chil. tecnol. méd; 9 (1), 1986

Se investiga la capacidad adherente y hemaglutinante de una cepa de E. COLI 36692 fimbriada y se compara con una cepa de E. COLI UCCSI no fimbriada. Mediante microscopía electrónica se demuestra la fibriación de E. COLI 36692, no observándose estas estructuras en E. COLI UCCSI. Se aísla el ADN extra...

Escherichia coli/genética, Escherichia coli/patogenicidad, Fimbrias Bacterianas/metabolismo, Pielonefritis/genética, ADN Bacteriano/análisis, Adhesión Bacteriana