Background: Freeze-drying is known as one of the best methods to preserve bacterial strains. Protectant is the key factor affecting the survival rate of freeze-dried strains. In addition, salinity, bacterial suspension concentration, drying time, and other factors can also affect the survival rate of str...
Pseudoalteromonas/fisiología,
Liofilización/métodos,
Preservación Biológica/métodos,
Viabilidad Microbiana,
Fenómenos Fisiológicos Bacterianos,
Supervivencia Celular,
Lactosa/química,
Disacáridos/química,
Manitol/química,
Salinidad,
Trehalosa/química
ABSTRACT Dengue has been a significant public health problem in Colombia since the simultaneous circulation of the four dengue virus serotypes. The replicative fitness of dengue is a biological feature important for virus evolution and contributes to elucidating the behavior of virus populations and vira...
Línea Celular,
Supervivencia Celular,
Células Cultivadas,
Colombia,
Virus del Dengue/genética,
Virus del Dengue/patogenicidad,
Citometría de Flujo,
Formazáns,
Hígado/citología,
ARN Viral/genética,
Valores de Referencia,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Serogrupo,
Sales de Tetrazolio,
Factores de Tiempo,
Ensayo de Placa Viral,
Replicación Viral/genética
OBJECTIVES: Hypoxia leads to endothelial cell inflammation, apoptosis, and damage, which plays an important role in the complications associated with ischemic cardiovascular disease. As an oxidoreductase, p66Shc plays an important role in the regulation of reactive oxygen species (ROS) production and apo...
Introdução: Novas formulações de materiais vítreos apresentaram atividade antimicrobiana e osteoindutora. Objetivos: O objetivo deste estudo foi avaliar a citotoxicidade e a biocompatibilidade de soluções produzidas a partir de novas formulações de biovidros, o F18 (biovidro experimental) e o F1...
Prasetyo, Eric Priyo;
Widjiastuti, Ira;
Cahyani, Febriastuti;
Kuntjoro, Mefina;
Hendrijantini, Nike;
Hariyani, Ninuk;
Winoto, Ervina Restiwulan;
Nugraha, Alexander Patera;
Goenharto, Sianiwati;
Susilowati, Helen;
Hendrianto, Eryk;
Rantam, Fedik Abdul.
Abstract Objective: To examine the cytotoxicity of calcium hydroxide on human umbilical cord mesenchymal stem cells (HUCMSC) to understand the characteristics for use in regenerative dentistry procedures especially regenerative endodontics. Material and Methods: HUCMSC was isolated, cultured, and confi...
BackgroundBreast cancer has been considered a public health problem and homeopathic treatments are becoming increasingly recommended due to its ways of action and absence of adverse effects. MCF-7 is an adenocarcinoma of human breast cell line useful as preclinicalmodel to screen ther...
Neoplasias de la Mama/patología,
Terapéutica Homeopática,
Viscum album/toxicidad,
Técnicas de Cultivo de Célula,
Adenocarcinoma/patología,
Células MCF-7/efectos de los fármacos,
Células Madre Mesenquimatosas/efectos de los fármacos,
Neoplasias de la Mama/tratamiento farmacológico,
Adenocarcinoma/tratamiento farmacológico,
Recuento de Células,
Supervivencia Celular
Abstract This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy-Δ12,14-PG J2 (15d-PGJ2), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGFβ-1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated wi...
Análisis de Varianza,
Supervivencia Celular/efectos de los fármacos,
Células Cultivadas,
Ensayo de Inmunoadsorción Enzimática,
Fibroblastos/efectos de los fármacos,
Fibroblastos/metabolismo,
Encía/citología,
Interleucina-6/metabolismo,
Lipopolisacáridos/farmacología,
Lectinas de Plantas/farmacología,
Prostaglandina D2/análogos & derivados,
Prostaglandina D2/farmacología,
Valores de Referencia,
Estadísticas no Paramétricas,
Factores de Tiempo,
Factor de Crecimiento Transformador beta1/efectos de los fármacos,
Factor de Crecimiento Transformador beta1/metabolismo
Abstract Autologous fibrin matrices derived from the Leukocyte and Platelet Rich Plasma (L-PRP) and Leukocyte and Platelet Rich Fibrin (L-PRF) techniques present great potential to act as a bioactive scaffold in regenerative medicine, contributing to the maintenance of cell viability, proliferation stimu...
Abstract Fluorescent nanodiamond (FND) has been used for long-term cell labeling and in vivo cell tracking because they have good at photostability and biocompatibility. In this study, we evaluate the effect of fluorescent nanodiamond labeling on in vitro culture and differentiation of human umbilical co...
BACKGROUND: Chinese hamster ovary (CHO) cells are the most commonly used mammalian host cell In the commercial-scale production of biopharmaceutical proteins. Modification of genes involved in apoptosis may improve the productivity of CHO cells. Executive caspases, including caspases 3 and 7, play critic...
