Results: 97

Molecular cloning, characterization and expression analysis of CpCBF2 gene in harvested papaya fruit under temperature stresses

Electron. j. biotechnol; 16 (4), 2013
Background: C-repeat binding factors (CBFs) are transcription factors that regulate the expression of a number of genes related to abiotic stresses. Few CBF genes have been cloned from other plants but no report in papaya. In present study, a full-length cDNA, designated as CpCBF2, was cloned from papaya...

Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation

Electron. j. biotechnol; 16 (4), 2013
Background: Enzymatic activity and laccase isoenzymes number of Pleurotus ostreatus grown in different pH values of the growing medium in submerged fermentation and incubated in buffer solutions of different initial pH values were determined. The expression profiles of five laccase genes (Lacc1, Lacc4, L...

Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples

OBJECTIVE: Adequate isolation of nucleic acids from peripheral blood, fine-needle aspiration cells in stained slides, and fresh and formalin-fixed/paraffin-embedded tissues is crucial to ensure the success of molecular endocrinology techniques, especially when samples are stored for long periods, or when...
ADN, ARN, ADN, ARN

Utilização de ácidos nucleicos fetais livres no plasma materno para o diagnóstico pré-natal: realidade do Brasil neste cenário

A descoberta de ácidos nucleicos fetais livres no plasma de gestantes possibilitou o desenvolvimento de novos testes de diagnóstico pré-natal não invasivo para a determinação do sexo e do Rh fetal. Esses testes foram implantados no sistema de saúde pública de diversos países da Europa há mais d...
ADN, ARN

Crecimiento somático y relación ARN/ADN en estadios juveniles de Eucinostomus argenteus (Pisces: Gerreidae) en dos localidades del Caribe de Venezuela

Rev. biol. trop; 60 (supl.1), 2012
Somatic growth and RNA/DNA rate of Eucinostomus argenteus (Pisces: Gerreidae) juveniles stages at two localities of the Venezuelan Caribbean. In order to evaluate the association among growth indices of marine fishes at early life stages, the somatic growth rate and physiological conditions of Eucinostom...

Avaliação da utilização do teste immunoblot recombinante (RIBA) no diagnóstico da infecção pelo vírus da hepatite c (VHC) em doadores de sangue com anti-VHC reagente

A infecção pelo vírus da hepatite C (VHC) é comumente assintomática e apresenta uma elevada taxa de cronicidade, podendo evoluir para cirrose e carcinoma hepatocelular. O diagnóstico da hepatite C é realizado através da pesquisa de anticorpos pelo teste de ELISA (Enzyme Linked Immunosorbent Assay...
ARN