Background: Agro-wastes were used for the production of fibrinolytic enzyme in solid-state fermentation. The process parameters were optimized to enhance the production of fibrinolytic enzyme from Bacillus halodurans IND18 by statistical approach. The fibrinolytic enzyme was purified, and the properties ...
A bacterial strain (PAP04) isolated from cattle farm soil was shown to produce an extracellular, solvent-stable protease. Sequence analysis using 16S rRNA showed that this strain was highly homologous (99%) to Brevibacillus laterosporus. Growth conditions that optimize protease production in this strain ...
Proteínas Bacterianas/biosíntesis,
Proteínas Bacterianas/química,
Brevibacillus/aislamiento & purificación,
Brevibacillus/metabolismo,
Bovinos,
Medios de Cultivo,
Electroforesis en Gel de Poliacrilamida,
Estabilidad de Enzimas,
Concentración de Iones de Hidrógeno,
Compuestos Orgánicos/metabolismo,
Péptido Hidrolasas/biosíntesis,
Péptido Hidrolasas/química,
Microbiología del Suelo,
Solventes,
Temperatura,
Factores de Tiempo
Background Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted a...
Background Laccase has been considered important for the degradation of lignocellulose by wood rot fungi. The properties and functions of laccase in white rot fungi have been investigated extensively, but those from brown rot fungi remain largely unknown. In this paper, a laccase isoform Pplcc2 from the ...
Cruz Gómez, Jhon Fredy;
Quintero Vega, Militza;
Bastidas, Marco;
Quintero, Willian;
Hernández, Danmaris;
Duque, Carlos;
Blanco, Natasha;
Rojas, Lisbet;
Guzmán, Reinaldo;
Solorzano, Marise;
Salcedo, Nol;
García, Nereyda;
Puig Pons, Juan.
OBJETIVO: estandarizar el uso de electroforesis horizontal en poliacrilamida como un método rápido de fácil implementación y de alta sensibilidad para la detección y tipificación del virus del papiloma humano por reacción en cadena de la polimerasa-polimorfismos de longitud de fragmentos de restri...
In the family of Euphorbiaceae, the genera Euphorbia and Sapium are known to contain essentially latex-bearing species. In the present study, the latex of Euphorbia selloi (Klotzsch & Garcke) Boiss., Euphorbia papillosa A.St.-Hil., and Sapium glandulosum (L.) Morong, plants native from Brazil, were e...
BACKGROUND: Liver regeneration (LR) after 2/3 partial hepatectomy (PH) is one of the most studied models of cell, organ, and tissue regeneration. Although the transcriptional profile analysis of regenerating liver has been carried out by many reserachers, the dynamic protein expression profile during LR ...
Hepatectomía,
Hígado/metabolismo,
Regeneración Hepática/fisiología,
Proteómica,
Proteínas 14-3-3/metabolismo,
Peso Corporal/fisiología,
Metabolismo de los Hidratos de Carbono/fisiología,
Metabolismo de los Lípidos/fisiología,
Hígado/anatomía & histología,
Biosíntesis de Proteínas/fisiología,
Transducción de Señal/fisiología,
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos,
Proteína de Unión al GTP cdc42/metabolismo,
Western Blotting,
Electroforesis en Gel Bidimensional,
Electroforesis en Gel de Poliacrilamida,
Distribución Aleatoria,
Ratas Sprague-Dawley,
Factores de Tiempo
BACKGROUND: Cronobacter sakazakii is considered as an emerging foodborne pathogen. The aim of this study was to isolate and characterize virulent strains of Cronobacter sakazakii from food samples of Bangladesh. RESULT: Six (6) Cronobacter sakazakii was isolated and identified from 54 food samples on the...
Cronobacter sakazakii/fisiología,
Microbiología de Alimentos,
Leche/microbiología,
Estrés Fisiológico/fisiología,
Antibacterianos/farmacología,
Cronobacter sakazakii/clasificación,
Cronobacter sakazakii/aislamiento & purificación,
Cronobacter sakazakii/patogenicidad,
ADN Bacteriano/análisis,
Fermentación/fisiología,
Leche/clasificación,
Reacción en Cadena de la Polimerasa/métodos,
Sideróforos/metabolismo,
Especias/microbiología,
Resistencia a la Tetraciclina/genética,
Bangladesh,
Reacciones Cruzadas,
Cartilla de ADN,
Electroforesis en Gel de Poliacrilamida,
Calor,
Concentración de Iones de Hidrógeno,
Análisis de Secuencia de ADN,
Virulencia
Background: Recombinant proteins, including antibodies and antibody fragments, often contain disulfide bond bridges that are necessary for their folding, stability and function. Production of disulfide-bond-containing proteins in the periplasm of Escherichia coli has been very useful, due to unique chara...
Total proteins and esterases from silk gland extracts of Bombyx mori silkworm were characterized and compared with electrophoretic profiles of prepared extracts with silkworm glands infected with nucleopolyhedrovirus (BmNPV). SDS-PAGE (7%) gels was used for the total proteins, and 10% native PAGE for est...
Bombyx,
Bombyx,
Bombyx,
Bombyx,
Nucleopoliedrovirus,
Nucleopoliedrovirus,
Nucleopoliedrovirus,
Nucleopoliedrovirus,
Isoenzimas,
Isoenzimas,
Isoenzimas,
Isoenzimas,
Electroforesis en Gel de Poliacrilamida,
Electroforesis en Gel de Poliacrilamida,
Electroforesis en Gel de Poliacrilamida,
Electroforesis en Gel de Poliacrilamida
