Results: 95

Human plasma fibronectin promotes proliferation and differentiation of odontoblast

J. appl. oral sci; 25 (3), 2017
Abstract Objective To assess the effect of fibronectin (Fn) and porcine type I collagen (PCOL) on odontoblast-like cells in vitro. Material and Methods Rat odontoblast-like cells (MDPC-23 cells) were inoculated and cultured on Fn-coated or type I collagen-coated substrates. Proliferation assay, alkal...

Cancer Stem Cells are Depolarized Relative to Normal Stem Cells Derived from Human Livers

Ann. hepatol; 16 (2), 2017
ABSTRACT Introduction and aim. The inability to distinguish cancer (CSCs) from normal stem cells (NSCs) has hindered attempts to identify safer, more effective therapies for hepatocellular carcinoma (HCC). The aim of this study was to document and compare cell membrane potential differences (PDs) of CS...

Analysis of clinical data and T helper 1/T helper 2 responses in patients with different clinical forms of leprosy

Abstract INTRODUCTION: Currently, there are no laboratory tests or sensitive and specific molecular markers for the early diagnosis of leprosy. The aim of this study was to analyze the clinical characteristics of patients with leprosy and investigate their immunological profile, comparing this with the ...

Primary cultures of mouse small intestinal epithelial cells using the dissociating enzyme type I collagenase and hyaluronidase

Braz. j. med. biol. res; 50 (5), 2017
The epithelium is a highly dynamic system, which plays a crucial role in the homeostasis of the intestinal tract. However, studies on the physiological and pathophysiological functions of intestinal epithelial cells (IECs) have been hampered due to lack of normal epithelial cell models. In the present st...

Tyrosine phosphorylation of Kv1. 5 is upregulated in intrauterine growth retardation rats with exaggerated pulmonary hypertension

Braz. j. med. biol. res; 50 (11), 2017
Intrauterine growth retardation (IUGR) is associated with the development of adult-onset diseases, including pulmonary hypertension. However, the underlying mechanism of the early nutritional insult that results in pulmonary vascular dysfunction later in life is not fully understood. Here, we investigate...

Inmunofluorescencia en tejidos fijados y preservados en parafina (IF-P). Una mirada desde la patología quirúrgica
Immunofluorescence labelling of paraffin-fixed and embedded tissue sections (IF-P). From the surgical pathology perspective

Repert. med. cir; 26 (4), 2017
La inmunofluorescencia en tejidos fijados y preservados en parafina (IF-P), al igual que la inmunohistoquímica (IHQ) y la inmunofluorescencia (IF), tiene como objetivo localizar antígenos a través de la unión antígeno-anticuerpo. Esta técnica es poco conocida en la patología quirúrgica, su utilid...

Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells

Biol. Res; 50 (), 2017
BACKGROUND: Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells. RESULTS: In thi...

Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments

J. appl. oral sci; 24 (5), 2016
ABSTRACT Objective: The aim of the present study was to evaluate and compare the cytotoxic effects of Biodentine and MTA on dental pulp stem cells (DPSCs) and to assess cell viability and adherence after material exposure to an acidic environment. Material and Methods: DPSCs were cultured either alone ...

The effects of riboflavin and ultraviolet light on keratocytes cultured in vitro

Arq. bras. oftalmol; 79 (3), 2016
ABSTRACT Purpose: To culture quiescent human keratocytes and evaluate the effects of ultraviolet light and riboflavin on human corneal keratocytes in vitro. Methods: Keratocytes were obtained from remaining corneoscleral ring donor corneas previously used in corneal transplant surgeries and cultured in...

Human herpesvirus 8 (HHV-8) detected by nested polymerase chain reaction (PCR) in HIV patients with or without Kaposi's sarcoma. An analytic cross-sectional study

Säo Paulo med. j; 134 (3), 2016
CONTEXT AND OBJECTIVE: Kaposi's sarcoma (KS) is a common neoplastic disease in AIDS patients. The aim of this study was to evaluate the frequency of human herpesvirus 8 (HHV-8) infection in human immunodeficiency virus (HIV)-infected patients, with or without KS manifestations and correlate HHV-8 detect...