Results: 951

Efecto antibacteriano in vitro del aceite esencial de Minthostachys mollis (muña) en Streptococcus mutans
In vitro antibacterial effect of the essential oil of Minthostachys mollis (muña) in Streptococcus mutans

El presente trabajo se evaluó el efecto antibacteriano in vitro del aceite esencial de Minthostachys mollis en Streptococcus mutans, la especie vegetal se recolectó de la ciudad de Tarma (Junín) aproximadamente 8 kilos de hojas de muña, que fueron procesados para la obtención del aceite esencial, re...

Evaluación del efecto del extracto de alcaloides totales de la Galipea longiflora (Krause) Evanta, sobre la actividad fagocítica de macrófagos humanos infectados con Leishmania braziliensis, octubre de 2010 y abril de 2011
Evaluation of the effect of total alkaloid extract of Galipea longiflora (Krause) Evanta, on the phagocytic activity of human macrophages infected with Leishmania braziliensis, October 2010 and April 2011

Durante el proceso de infección natural, Leishmania toma contacto con una variedad de fagocitos mononucleares en los diferentes tejidos incluyendo macrófagos residentes y monocitos que se dirigen hacia el sitio de infección desde la circulación sanguínea, en este trabajo utilizamos macrófagos human...

Actividad antioxidante y antiinflamatoria del extracto hidroalcohólico de las flores de Tanacetum parthenium L. Sch. Bip. "Santa María". Ayacucho, 2014
Antioxidant and anti-inflammatory activity of the hydroalcoholic extract of the flowers of Tanacetum parthenium L. Sch. Beep. "Santa Maria". Ayacucho, 2014

Los procesos inflamatorios no controlados producen daño celular donde los radicales libres están presentes y representan un peligro para la vida del paciente, en este contexto la búsqueda de moléculas de origen vegetal son incesantes por su aceptación cultural, una mejor compatibilidad con el cuerpo...

Desenvolvimento de sorvete simbiótico de graviola ( Annona muricata L. ) com teor reduzido de gordura e avaliação da resistência gastrointestinal dos probióticos in vitro

O presente trabalho objetivou avaliar o efeito da adição de inulina (I) e a substituição parcial da gordura do leite (G) pelo concentrado de proteína de soro de leite (WPC) sobre a sobrevivência dos probióticos Lactobacillus acidophilus NCFM e Bifidobacterium animalis subsp. lactis HN019 em sorvet...

Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker

Electron. j. biotechnol; 16 (6), 2013
Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum. Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) ...

Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana

Electron. j. biotechnol; 16 (6), 2013
Background: Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic Arab...

Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line

Electron. j. biotechnol; 16 (6), 2013
Background: Transgenesis by microinjection has been widely used for the generation of different mouse models. Different variables of the procedure may critically affect the efficiency of the process. A DNA construction that carries the CXCL2 promoter gene and firefly luciferase has been used to optimize ...

Efeito da aplicação tópica de um verniz de TiF4 quimicamente estável na desmineralização do esmalte dentário bovino: estudo in vitro

Objetivo: Avaliar, in vitro, o efeito preventivo da aplicação tópica de um verniz à base de tetrafluoreto de titânio quimicamente estável e de um verniz à base de fluoreto de sódio sobre o esmalte bovino, mediante ciclagem de pH. Metodologia: A amostra foi constituída por 75 blocos de esmalte bo...

Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers

Electron. j. biotechnol; 16 (5), 2013
Background: Broccoli, Brassica oleracea subsp. italica is one of the many valuable Brassica species which is still less cultured under in vitro condition. Heat tolerant transgenic and non-transgenic broccoli cv. Green Marvel plantlets with well-developed root system obtained through in vitro culture were...

Ethylene treatment improves diosgenin accumulation in in vitro cultures of Dioscorea zingiberensis via up-regulation of CAS and HMGR gene expression

Electron. j. biotechnol; 16 (5), 2013
Background: The perennial medicinal herb Dioscorea zingiberensis is a very important plant used for steroid drug manufacturing for its high level of diosgenin in rhizome. Although the stimulation of diosgenin accumulation by ethylene has been reported in a few of plant species, its regulation is not yet ...